パスツレラ症により死亡したウサギの病理学的解析及び分離菌<i>Pasteurella multocida</i> 血清型-:1の遺伝的解析

Abstract

In a rabbit breeding facility, 10 rabbits died successively within a week. Upon the pathological examination of a rabbit, necrotizing hemorrhagic bronchopneumonia was found. Pasteurella multocida was isolated from the lung lesion. A capsular typing polymerase chain reaction of the isolate yielded both types A-and F-specific amplification products. DNA sequencing of its capsule biosynthetic loci revealed that the isolate preser ved all sequences of types A and F primer design sites. However, the capsular serotype of the isolate could not be determined by an indirect hemagglutination reaction. The isolate was classified into sequence type (ST) 4 using multilocus sequence typing following the Rural Industries Research and Development Corporation (RIRDC) scheme. This is the first repor t on the isolation from a rabbit of a P. multocida grouped into clonal complex 171 consisting of ST4,