Экстратимическая Дифференцировка И Антигенраспознающая Активность ΑβТ-Лимфоцитов При Беременности У Мышей

Abstract

We studied reactivity of alphabetaT lymphocytes in CBA pregnant females toward male antigens and the presence of gene rearrangement in T-cells antigen receptor in peripheral lymphoid organs of mice in the case of three breeding variants: CBA x BALB/c (normal allogenic pregnancy), CBA x CBA (syngenetic pregnancy), and CBA x DBA/2 (prone to abortion combination). It was shown that proliferative response of alphabetaT lymphocytes in pregnant CBA females to male spleen cells was the most marked at normal allogenic pregnancy, the least marked at syngenic pregnancy, and was not observed at the combination CBA x DBA/2. In addition, cells ofparaaortic lymphatic nodes (draining uterus) respond to male antigen reliably more effectively than lymphocytes in mesenterial and axillary lymphatic nodes. Simultaneous estimation of recombinase RAG-1, the key enzyme in rearrangement of T-receptor genes, revealed similar principles: predominant activity of recombinase in T lymphocytes in paraaortal lymphatic nodes of CBA pregnant females. This points to the relationship between extrathymic rearrangement of antigen receptor genes and change in the antigen-detecting repertoire of these cells. The possible biological significance of the discovered phenomenon is discussed.