Сравнительная Оценка Экспрессии Гена Toll-Подобного Рецептора-2 В Слизистой Оболочке Верхних Дыхательных Путей

Tyrnova, E. V.; Алешина, Г. М.; Янов, Ю К; Кокряков, В.Н.

doi:10.1134/s0869813918120105

Cited by 2 publications

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“…Синтез первой цепи комплементарной ДНК (кДНК) проводили с использованием обратной транскриптазы M-MLV (Promega, США) в присутствии oligo(dT) и dNTPs (Медиген, РФ). Амплификацию проводили с использованием специфических праймеров hBD-3 (прямого 5´-tatcttctgtttgctttgctcttcc-3' и обратного 5´-cctct gactctgcaataatatttctgtaat-3') и b-актина человека (прямой праймер 5'-gggtcagaaggattcctatg-3', обратный 5'-ggtctcaaacatgatctggg-3') [3]…”

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Human β-defensin-3gene expression in mucosa of ORL organs

2022

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The aim of present study was to investigate the hBD-3 gene expression in the surface epithelium of mucosa in ORL organs. We have studied a total of 210 mucosal samples, obtained at the most frequent surgical intervantions from 5 different anatomical functional areas: nose and paranasal sinuses, middle ear, nasopharynx, oropharynx, larynx. The inferior turbinate mucosa (1) and the normal middle nasal passage mucosa (2) served as controls. Estimation of hBD-3 and β-actin gene expression was performed by reverse transcription and realtime PCR. In the nasal and sino-nasal mucosa, only negligible expression levels were detected in 14.29-33.33% of samples, most often in the specimens from the middle nasal passage and ethmoid labyrinth polyps (53.84%), being absent in hypertrophic inferior turbinate. In the middle ear cavity, the frequency detection of the hBD-3 gene expression varied from 7.69% in the stapes superstructures mucosa to 53.85% of the mucosal samples in the presence of cholesteatoma. hBD-3 gene expression was detected in most tissue samples with high microbial contamination: palatine tonsils (100%); adenoid hypertrophy (84.62%); adenoids in hypertrophic states of adenoids and palatine tonsils (87.5%); laryngeal fibrous-vascular polyps (87.5%); other laryngeal pathology (77.78% of the samples). The highest levels of hBD-3 gene expression were found in laryngeal fibrous-vascular polyps. The findings presumed two functionally different types of immune response in mucosa of the ORL organs. In the anatomical-functional areas lined with ciliated epithelium (middle and inferior nasal passages, maxillary and ethmoid sinuses, middle ear), significantly lower frequencies (Fisher's exact test, p < 0.05 to p < 0.001) and levels (Mann-Whitney test, p < 0.05 to p < 0.001) of hBD-3 gene expression were detected, except of polyps of the middle nasal passage and ethmoid labyrinth, and mucosa of the tympanic cavity in cholesteatoma, which may be related to the nature of the pathological process. In the areas lined with squamous epithelium or a combination of squamous and ciliated epithelium, hBD-3 gene expression was detected almost everywhere and at significantly higher levels. In the context of chronic inflammation and infection-related diseases of the ORL organs, in addition to the direct microbicidal activity of hBD-3 as the first line of immune response, one may suggest peptide dysregulation and, even, pathogenetic effects of hBD-3, e.g., increased sensitivity to infections, pathological changes in the composition of the commensal bacteria, fibrous remodeling.

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Section: материалы и методыunclassified

Human β-defensin-3gene expression in mucosa of ORL organs

2022

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The role of antimicrobial defense factors in the pathogenesis of immune disorders in deviated septum

Kornova

2021

Terapevt (General Physician)

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The article presents the data from Russian and foreign literature on the state of humoral and cellular factors of local antimicrobial protection of the mucous membrane of the nasal cavity and paranasal sinuses in the pathogenesis of immune disorders in deviated septum. The role of cellular, humoral factors, and interreceptor interactions of cells of the respiratory, glandular epithelium, phagocytes, lymphocytes in pathological conditions of the nasal mucosa and paranasal sinuses was analyzed. The role of immune mechanisms in the development of pathological reactions in deviated septum is shown. The presented review will expand the understanding of the role of antimicrobial factors in the pathogenesis of immune disorders in deviated septum.

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Сравнительная Оценка Экспрессии Гена Toll-Подобного Рецептора-2 В Слизистой Оболочке Верхних Дыхательных Путей

Cited by 2 publications

References 0 publications

Human β-defensin-3gene expression in mucosa of ORL organs

Human β-defensin-3gene expression in mucosa of ORL organs

The role of antimicrobial defense factors in the pathogenesis of immune disorders in deviated septum

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