Сorrelation between the number and aggregation capacity of platelets and the process of lipid peroxidation in patients with chronic hepatitis С

Abstract

The purpose — to determine the role of the relationship between the number and aggregation capacity of platelets (Tr) with the process of lipid peroxidation (POL) in the natural course of chronic hepatitis C (HCV). Material and methods. 332 patients with HCV were observed, divided into three groups according to the phases of disseminated intravascular coagulation and the number of Tr: the first group — the hypercoagulation phase (Tr > 330 x 109/l, n = 22); the second — the transition phase (Tr from 330 to 200 x 109/L, n = 132); the third — hypocoagulation (Tr < 200 x 109/L, n = 178). The vascular-platelet link of hemostasis was evaluated by the number and degree of maximum amplitude (MA) of spontaneous Tr aggregation. The POL process was studied by medium-weight molecules (MSM), malonic dialdehyde (MDA), and enzymes — superoxide dismutase (SOD), reduced (VG) and oxidized (OG) glutathione. Results. The decrease in the number of Trs against the background of prolonged infection with the hepatitis C virus was accompanied by a decrease in the antioxidant potential, an increase in the intensity of lipoperoxidation and an increase in the ability of Tr to aggregate, especially pronounced in the phase of hypocoagulation. Correlations were revealed: the numbers Tr showed inverse correlation with the levels of SOD, MSM, MDA, OG and direct correlation with VG; MA of spontaneous aggregation Tr showed direct correlation with MSM, MDA, SOD, OG and inverse correlation with VG. Conclusions. Changes in the number and aggregation ability of Tr, the level of POL products in the course of the natural course of HCV, in parallel with the ALT enzyme, reflect the activity of the infectious process. The number and MA of spontaneous Tr aggregation in patients with natural HCV with exacerbation of the disease correlate with a reduced antioxidant potential and increased intensity of lipoperoxidation. The natural course of HCV is characterized by a multidirectional change in the AOS enzymes – an increase in the SOD level and a decrease in the amount of reduced glutathione with a simultaneous increase in the oxidized one.