Сomparative evaluation of sperm DNA fragmentation and other sperm parameters

Zhabin, S. G.; Trechenkov, E. A.; Артифексов, С. Б.; Pavlenko, I.I.; Polukarov, A. N.

doi:10.17116/repro2015214121-124

Cited by 3 publications

(2 citation statements)

References 19 publications

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“…Giannoccaro et al noted a weak correlation between DNA fragmentation in stallion spermatozoa and their mobility and viability [15]. At the same time, other authors reported a negative effect of chromatin damage on the motility of human spermatozoa [16,17] and their fertilizing ability [1]. Our data support these observations, although the correlation between DNA fragmentation and either sperm motility or survival was more pronounced for thawed sperm than for the fresh one.…”

Section: Discussionsupporting

confidence: 84%

Reproductive Characteristics of Thawed Stallion Sperm

Atroshchenko

Arkhangelskaya

Isaev

et al. 2019

Animals

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The main goal of our study was to determine a set of thawed stallion sperm characteristics that have predictive value for the pregnancy rate (PR) of mares after artificial insemination (AI). DNA fragmentation and survival of sperm during hypothermic storage were studied in addition to routinely determined semen characteristics such as concentration, percentage of motile spermatozoa, and morphology. To estimate DNA fragmentation, a modified hallo assay was applied. Sperm survival was determined within hours as the ability of spermatozoa to maintain progressive motility (PM) during the storage of ejaculate diluted with lactose-chelate-citrate-yolk (LCCY) medium at +4 °C. Strong positive correlation between PR and thawed sperm motility (r = 0.90, p < 0.05) as well as between PR and sperm survival (r = 084, p < 0.05) was revealed. There was also a strong negative correlation between PR and DNA damages in spermatozoa (r = −0.94, p < 0.05). We found no dependence of PR on normal morphology spermatozoa percentage in thawed semen. We concluded that the sperm activity, survival, and DNA fragmentation should be considered as the sufficient reproductive characteristics of semen to evaluate the quality of frozen/thawed sperm and prediction of PR.

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Section: Discussionsupporting

confidence: 84%

Reproductive Characteristics of Thawed Stallion Sperm

Atroshchenko

Arkhangelskaya

Isaev

et al. 2019

Animals

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“…The structure of spermatozoa in the hybrids may be abnormal, as well: tail deformation (twisting or angular deformation) and a higher proportion of spermatozoa without a head. These changes can lead to the loss of the abilities to fertilize and to advance in the female genital tract [46].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the “Bottleneck” Effect in an Isolated Population of Microtus hartingi (Rodentia, Arvicolinae) from the Eastern Rhodopes (Bulgaria) by Methods of Integrative Analysis

et al. 2022

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An integrative analysis of an isolated population of Harting’s vole (Microtus hartingi) from the Eastern Rhodope Mountains (Bulgaria) was carried out by morphological and morphometric methods, computed tomography, Cytb variation data, and experimental hybridization. Substantial changes in the development of the skull and teeth were found. Nevertheless, those voles can live to the senex stage. A phylogenetic reconstruction based on Cytb sequences showed that M. hartingi from the Eastern Rhodopes forms a separate clade, which is a sister clade to the voles from Northeastern Greece (also from the foothills of the Rhodopes). M. hartingi from the Rhodopes is mostly isolated reproductively from M. h. ankaraensis from Anatolia (Turkey), as evidenced by a decrease in the proportion of pairs that started breeding, relatively high mortality of pups, and increased sterility of hybrid males. Possible time of isolation of the Rhodopean population and consequences of the “bottleneck” effect on its current state are discussed. At the same time, we believe that, at present, it is impossible to determine its taxonomic status. It is necessary to recognize M. hartingi from the Bulgarian Rhodopes as an endangered population and to include it in regional Red Lists of Bulgaria.

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Automated and Manual Semen Analysis: The Comparative Characteristics

et al. 2022

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Currently, spermogram parameters are routine criteria for assessing male fertility. There are two methods of semen analysis: traditional manual and automated. The concentration of spermatozoa of various motility categories is important in predicting physiological conception, as well as in choosing fertilization methods in assisted reproductive technology programs. The introduction of automatic semen analyzers has shown that their use can be considered as an alternative to the routine manual analysis method, which can contribute to laboratory standardization. Initially, these devices demonstrated difficulty in accurately indicating sperm concentration due to the presence of sperm aggregation and large amounts of cellular debris. In the present study, the main focus was on the analysis of sperm concentration by manual and automated methods. A total of 50 sperm samples were analyzed from patients participating in assisted reproductive technology programs. Manual analysis was performed in a Makler's chamber according to the standard method in 10 small squares. The motility of each spermatozoon was classified into categories. Automated analysis was carried out using the CASA sperm analyzer computer analysis system (MICROPTIC, Spain), which uses the principle of microscopic imaging and processing to detect motile and immobile spermatozoa through fast and consistent images. Statistical methods for independent variables were chosen to evaluate the obtained data. As part of the study, by the method of determining confidence intervals, a statistically significant difference was found between automated and standard or manual methods of analysis when evaluating spermatozoa with the highest speed of 0,025 mm/sec and rectilinear and translational movement, as well as with a lower speed, either aging or with broken morphology. Most likely, this is due to the objective difficulty of visual assessment of spermatozoa of such motility categories. The data obtained suggest that automated analysis has a higher degree of objectivity in assessing mobile biological objects, in particular male germ cells.

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Сomparative evaluation of sperm DNA fragmentation and other sperm parameters

Cited by 3 publications

References 19 publications

Reproductive Characteristics of Thawed Stallion Sperm

Reproductive Characteristics of Thawed Stallion Sperm

Evaluation of the “Bottleneck” Effect in an Isolated Population of Microtus hartingi (Rodentia, Arvicolinae) from the Eastern Rhodopes (Bulgaria) by Methods of Integrative Analysis

Automated and Manual Semen Analysis: The Comparative Characteristics

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