Рredominant microflora of natural and wastewaters of Lviv region

Koval, Iryna

doi:10.23939/ctas2020.02.121

CTAS

2020

DOI: 10.23939/ctas2020.02.121

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Рredominant microflora of natural and wastewaters of Lviv region

Iryna Koval¹

Abstract: Quantitative and qualitative composition of microorganisms have been determined by the microbiological methods of the analysis of natural waters and industrial wastewater. It is shown that the dominant microflora of the studied waters are Diplococcus, Sarcina, Bacillus, Pseudomonas bacteria types, blue-green algae of Oscillatoria types, as well as Saccharomyces yeast types. Morphological, physiological and cultural characteristics of colonies grown on nutrient media were studied to identify microorganisms. The… Show more

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2021

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The Influence of Helium And Cavitation on the Yeast Life Process

2021

Visnyk of v. N. Karazin Kharkiv National University Series "Ecology"

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Purpose of the study is to іnvestigate the simultaneous effect of cavitation and helium on the viability of yeast of the genus Saccharomyces cerevisiae in water. To study the change in the number of cells during cavitation treatment of the water system in the gas atmosphere. Methods. Yeast of Saccharomyces cerevisiae type were used as test microorganisms. Freshly prepared distilled desaerated water was used for the research, to which yeast cells were introduced with a microbiological loop. The volume of the model medium was cooled in a glass reactor with tap water, the temperature of which corresponded to 298 ± 1 K. The total duration of the process was 2 hours. The cavitation source was an ultrasonic generator UZDN-2T with frequency of 22 kHz and power of 35 W. The test water was bubbled with gas throughout the process. The test gas was helium. The number of microorganisms per unit volume of test water was determined by the total number of colonies on the nutrient medium on Petri dishes and expressed in colony-forming units (CFU). Results. In the experimental part of the work the process of water treatment with the content of yeast cells under cavitation conditions with simultaneous supply of helium is proposed. The efficiency of water purification from yeast as a result of the combined action of helium/cavitation has been established. The value of the effective rate constant of microorganisms destruction according to the kinetic reaction equation of the first order is calculated. The viability of yeast under cavitation conditions and bubbling of helium through the water system has been studied. The proportion of destroyed cells during the two-hour action of yeast-contaminated water at different treatment regimes was calculated and compared. An active decrease in the number of Saccharomyces cerevisiae in the aqueous medium at the beginning of the process with the achievement of the proportion of destroyed cells (Dd) 40.48% after 30 min of combined He/cavitation action at the initial microbiological water contamination of 4.2 · 103 CFU/cm3. NMend = 100 CFU/cm3 after 90 minutes of water treatment, that corresponds to the water purification degree > 97%. The end result is almost pure water, which allows to discharge treated water into natural water. Conclusions. The obtained results indicate intensive cavitation purification of water from the studied microorganisms in the experimental conditions. The influence of the studied gas nature on the process of destruction of microbiological contaminants in water is described.

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The Influence of Helium And Cavitation on the Yeast Life Process

2021

Visnyk of v. N. Karazin Kharkiv National University Series "Ecology"

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Effectiveness of Using Gases for Water Purification with the Same Microbial Load

Koval

2021

Visnyk of v. N. Karazin Kharkiv National University Series "Ecology"

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Purpose of the study is to іnvestigate the effect of gases on the process of water purification from aerobic microorganisms (MO) with the same initial content in the aquatic medium and to identify the nature of the gas in the atmosphere in which the highest degree of destroyed microbial cells per unit volume of water is achieved. Methods. Aerobic bacteria of the Bacillus cereus bacteria type with the initial content of 7 · 104 CFU/cm3 were used for research. The investigated water was model water created on the basis of distilled deaerated waterwith the introduction of bacteria of a specific species. Oxygen, carbon dioxide, as well as inert - argon and helium were used as a studied gases. Process conditions were: duration - 2 hours, gas bubbling rate - 0.2 cm3/c, T = 288 ± 1 K. Has been used a quantitative method of counting the initial and final number of microorganisms (NM) by sowing samples of test water on nutrient medium in Petri dishes before and after experiments. Results. The different efficiency for the water purification process depending on the gas nature is shown. The NM value change of the time of bubbling gases is presented. The degree of destruction of the studied microorganisms was calculated depending on the mode of water treatment and the process duration. The oxygen influence on the process of MO destruction consists of two stages - a slight accumulation of cells (I stage) and their subsequent destruction in the II stage. The percentage of cell accumulation during 3600s during oxygen bubbling into the aqueous medium was 9.43%, which is due to the consumption of bubbling oxygen by bacteria. This led to the lowest process efficiency for the oxygen action in the end of the result (Dd is 34.73% only). The greatest efficiency of MO destruction was found during carbon dioxide bubbling (Dd = 91.0%), which is obviously due to the increase in the acidity of the test medium (pHinitial = 6.1 and pHend = 4.3). The influence of helium on water purification at different microbial load has been studied. It has been shown that the efficiency of cell destruction increases with a decrease of its number per unit volume of water. Conclusions. The influence of the different gases nature on the destruction process of aerobic MO in water with the same and different microbial load is shown. It is established that the process of water purification from MO depends on the nature of the bubbled gas. The gas, the supply of which allowed to achieve the largest number of destroyed cells after 7200 s, was experimentally determined. The highest efficiency of water disinfection is achieved during the action of carbon dioxide. A relative series of effective action of the investigated gases on microbial water purification is established.

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Рredominant microflora of natural and wastewaters of Lviv region

Cited by 2 publications

References 2 publications

The Influence of Helium And Cavitation on the Yeast Life Process

The Influence of Helium And Cavitation on the Yeast Life Process

Effectiveness of Using Gases for Water Purification with the Same Microbial Load

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