Морфофункциональная Характеристика Каллусов Ячменя, Толерантных К Токсическому Действию Алюминия

Баранова, Е. Н.; Чабан, И. А.; Кононенко, Н. В.; Shupletsova, O. N.; Широких, И. Г.; Поляков, В. Ю.

doi:10.7868/s0233475515030032

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…В целом направление получения регенерантов злаков каллусного происхождения, толерантных к различным экологически неблагоприятным абиотическим факторам, достаточно актуально в мировой биотехнологической практике. Так, исследователи активно разрабатывают способы получения каллусов in vitro и каллусных регенерантов ex vitro, устойчивых к воздействию солей, в том числе солей тяжелых металлов, низкой температуры, гипоксии/аноксии и других стресс-факторов [16][17][18][19][20][21][22][23][24].…”

unclassified

Callus culture in vitro in the experimental evaluation of drought resistance in cereals (review)

Круглова¹,

Seldimirova²,

Zinatullina³

2021

TVAN

View full text Add to dashboard Cite

One of the directions in plant biotechnological research is the use of callus cultures in vitro. The callus is an integrated system that is formed both exogenously (as a result of the proliferation of surface cells of various tissues of the plant organism) and endogenously (in the depth of these tissues). Initially, callus consists of homogeneous cells gradually transformed into a system of groups of heterogeneous cells with specific morphogenetic potencies. These potencies are realized by various ways of morphogenesis in vitro, including those leading to the formation of full-fledged plants-regenerants. In this review, using cereals as an example, we analyze the literature and our data on the use of calli in vitro as experimental systems for studying the plant stress-resistance to abiotic factors, especially to the drought factor. The advantages and limitations of using callus cultures in vitro are considered. The prospects of studying the mechanisms of action of abiotic stressors and resistance to them at the cellular and tissue levels under model conditions of callus culture in vitro are shown. Attention is paid to the assessment of the drought resistance of cereals under selective conditions simulating drought in vitro by such a parameter as the growth activity of calli. The issue of studying anti-stress effects in callus cultures in vitro is analyzed on the example of brassinosteroids. The prospects of using the model system “immature embryo in vivo–embryonic morphogenic callus in vitro” for rapid assessment of the effect of anti-stress plant growth regulators are considered. It is emphasized that the basis for using calli as the model system is both the significant role of the cell in all morphogenetic events of the plant organism in vivo and in vitro and the similarity of the responses of plants in vivo, calli in vitro, regenerants of callus origin in vitro and ex vitro.

show abstract

unclassified