Мониторинг Изменения Содержания Кислорода В Тканях По Кинетике Замедленной Флуоресценции Экзогенных Красителей

Abstract

The dynamics of oxygen consumption during photodynamic processes and its subsequent restoration in malignant tumors and healthy tissues of mice were studied in vitro by kinetics of long-term luminescence of xanthene dyes. It was shown that to estimate changes in tissue oxygen tension, specific type of delayed fluorescence can be used which caused by singlet-triplet annihilation of singlet oxygen and a sensitizer in triplet state. In tumors under pulse excitation of sensitizers, reversible quenching of delayed fluorescence was observed, which is associated with a decrease in the tissue oxygen tension during photodynamic processes. Method for visualizing the restoration of the initial oxygen level in tissues after photodynamic action is proposed.