The purpose of the research was to study in a comparative aspect the bioelemental status of sires of Kholmogorskaya breed under different natural and climatic conditions after a long winter period of using. The work was performed at the Federal Research Center for Animal Husbandry named after Academy Member L. K. Ernst, Russian State University of National Economy named after V. I. Vernadsky and on the basis of JSC “Head Center for Reproduction” (Moscow region) and JSC “Arkhangelsk Bull Stud” (Arkhangelsk region). In the experiment the sires of JSC “Arkhangelsk Bull Stud” (n = 6) and JSC “Head Center for Reproduction” (n = 5) were used. Biochemical parameters of blood were studied such as total protein, albumin, globulin, albumin-globulin ratio, total cholesterol, creatinine, urea, ALT, AST, total bilirubin, as well as macronutrients: calcium, phosphorus, calcium-phosphorus ratio, and trace elements: iron, magnesium, chlorides, copper, zinc, and alkaline phosphotase and glucose in a comparative aspect. A comparison of protein and lipid metabolism in breeding bulls, depending on the region, showed the following results. The diff erence between the sires of JSC “Head Center for Reproduction” and JSC “Arkhangelsk Bull Stud” in total protein was 8,75 g/l or 10,2 %, albumin by 27,5 %, total cholesterol by 42,2 %, creatinine by 11,5 %, urea by 58,8 %, ALT by 8,8 %, total bilirubin by more than 200 % in favor of the sires of JSC “Head Center for Reproduction”, the ratio between A/G by 32,6 %. However, signifi cant differences (p < 0,05) were found only in the content of urea and total bilirubin. It was concluded as a result of the research that the sires of JSC “Arkhangelsk Bull Stud” need to balance the ration in protein. and mineral content. In order to determine the needs of breeding bulls for essential elements, they should be monitored regularly, at least twice a year in blood serum taking into account the defi ciency or excess intake into the body according to the results of chemical analysis of water, soil and basic feeds, and compensated with targeted premixes.