Η Επίδραση Του Νεκρωτικού Των Όγκων Παράγοντα-Άλφα (TNF-α) Στην Φλεγμονή Του Πολφού

Abstract

Intentional exposure of pulp to the environment of the buccal cavity usually results in inflammation of variable degree. The objective of this study was to test whether the analysis of TNF-α gene expression with RT-PCR can assist in the monitoring of such changes, in canine teeth. Pulp tissue from 24 teeth, from a total of 5 animals, was used. Exposure was effected by preparation of cervical cavities by a round bur and lasted for 2 (n = 8) or 7 (n = 8) days. Pulp from unopened teeth (n = 8) served as the control. TNF-α gene expression was practically undetected -with an RT-PCR method -in either exposed or unexposed pulp, under the conditions used which did allow the detection of TNF-α mRNA in a positive control of inflamed tissue.We conclude that analysis of TNF-α gene expression with conventional RT-PCR cannot be used as a marker of short-to-medium term pulp tissue changes accompanying intentional pulp exposure in canine teeth.