ε-Polylysine and next-generation dendrigraft poly-L-lysine: chemistry, activity, and applications in biopharmaceuticals

Chen, Shi; He, Yuefeng; Feng, Xiaobo; Fu, Dehao

doi:10.1080/09205063.2015.1095023

Cited by 63 publications

(32 citation statements)

References 107 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, ε‐PL is not toxic towards humans (Zinoviadou et al ., ). Based on these properties, ε‐PL and its derivatives have a variety of applications in many fields, such as food industries, medicine and pharmaceuticals (Cai et al ., ; Shi et al ., ). However, ε‐PL has poor antioxidant property, which impedes its industrial applications.…”

Section: Introductionmentioning

confidence: 99%

Preparation and characterisation of novelty food preservatives by Maillard reaction between ε‐polylysine and reducing sugars

Zeng

Brennan

et al. 2019

Int J of Food Sci Tech

View full text Add to dashboard Cite

In order to enhance the application scope and usage effect of e-polylysine, it was modified by Maillard reaction through endowing antioxidant activity. Characterisations of different type of reaction systems were investigated. Results showed that with increased heating treatment time at constant 90°C and UV-Vis absorbance at 294 and 420 nm, fluorescence intensity of modified products were increased. DPPH radical scavenging activity of modified products from D-glucose (D-Glc), D-fructose (D-Fru) and Dxylose (D-Xyl) was increased to 40.5%, 44.0% and 69.4%, and the reducing power was increased to 0.9, 1.0 and 1.3 respectively (P < 0.05). Moreover, compared with e-polylysin, three types of modified products have similar antibacterial property. In addition, three types of modified products showed low cytotoxicity against the cultured human HepG2 cells in vitro. Therefore, the modified products derived from e-polylysine model could be used as potential food preservatives. Preparation and characterisation of food preservatives Z.-H. Zhang et al. 1825 Preparation and characterisation of food preservatives Z.-H. Zhang et al.

show abstract

Section: Introductionmentioning

confidence: 99%

Preparation and characterisation of novelty food preservatives by Maillard reaction between ε‐polylysine and reducing sugars

Zeng

Brennan

et al. 2019

Int J of Food Sci Tech

View full text Add to dashboard Cite

show abstract

“…Polylysine is a biocompatible, nontoxic polyamino acid with a broad spectrum of usage [20]. PLLs with molecular weights of 15,000-30,000 were usually used in the study of DNA modification, as shorter-chain PLL was shown to have low binding efficiency with DNA [27].…”

Section: Discussionmentioning

confidence: 99%

“…We previously produced a recombination protein of ε immunoglobulin heavy chain fused with PLL selectively targeting the allergen gene to human dendritic cells (DCs) [19]. PLL can electrostatically bind the negatively charged DNA and has extensively been used in drug delivery [20]. Since PLL is not only water soluble and biodegradable but can also prevent conjugated DNA from nuclease degradation and increase the transport efficiency of DNA, it has been shown to be an effective gene vector which makes DNA absorption easy, is presented by APCs, and improves the immunogenicity of DNA vaccine.…”

Section: Introductionmentioning

confidence: 99%

Enhanced Prophylactic and Therapeutic Effects of Polylysine-Modified Ara h 2 DNA Vaccine in a Mouse Model of Peanut Allergy

Zhu

Yu²,

Niu³

et al. 2016

Int Arch Allergy Immunol

View full text Add to dashboard Cite

Background: The prevalence of food allergy has been increasing, but treatment is very limited. DNA vaccination has been recognized as a promising method for the treatment of allergic diseases; however, poor immunogenicity has hindered its application. Methods: BALB/c mice were intradermally injected with plasmid DNA encoding the peanut protein Ara h 2 (pAra h 2) or pAra h 2 pretreated with poly-L- lysine (PLL) before or after sensitization with Ara h 2 protein. Ara h 2-specific antibodies were measured by ELISA. CD207+ dendritic cells (DCs) and Treg cells in draining lymph nodes were analyzed by flow cytometry after DNA immunization, and cytokine production in splenocytes was also analyzed. Results: In the prophylactic study, pretreatment with pAra h 2 or PLL-pAra h 2 resulted in lower levels of Ara h 2-specific IgG1, IgG2a, and IgE after sensitization with Ara h 2 protein, and mice in the PLL-pAra h 2 group had a significantly lower level of antibodies than those in the pAra h 2 group. In the treatment study, intradermal injection with pAra h 2 or PLL-pAra h 2 after Ara h 2 protein sensitization significantly decreased the level of Ara h 2-specific antibodies, and PLL- pAra h 2 had stronger effects than pAra h 2. There were increased numbers of CD207+ DCs and Treg cells in the mice receiving intradermal injection with PLL-pAra h 2, and splenocytes from PLL-pAra h 2-treated mice secreted increased levels of IFN-γ and IL-10. Conclusions: Modification of pAra h 2 with PLL improved its prophylactic and therapeutic effects in peanut-allergic mice.

show abstract

“…There are two kinds of PLL: i.e. α-PLL that is synthesized by artificial means and shows intrinsic toxicity, and ε-PLL that is synthesized using microbial means, having wide application in medicinal, electronics and food [40,41,42,43].…”

Section: Types Of Ph-responsive Polypeptidesmentioning

confidence: 99%

pH-Responsive Polypeptide-Based Smart Nano-Carriers for Theranostic Applications

et al. 2019

View full text Add to dashboard Cite

Smart nano-carriers have attained great significance in the biomedical field due to their versatile and interesting designs with different functionalities. The initial stages of the development of nanocarriers mainly focused on the guest loading efficiency, biocompatibility of the host and the circulation time. Later the requirements of less side effects with more efficacy arose by attributing targetability and stimuli-responsive characteristics to nano-carriers along with their bio- compatibility. Researchers are utilizing many stimuli-responsive polymers for the better release of the guest molecules at the targeted sites. Among these, pH-triggered release achieves increasing importance because of the pH variation in different organ and cancer cells of acidic pH. This specific feature is utilized to release the guest molecules more precisely in the targeted site by designing polymers having specific functionality with the pH dependent morphology change characteristics. In this review, we mainly concert on the pH-responsive polypeptides and some interesting nano-carrier designs for the effective theranostic applications. Also, emphasis is made on pharmaceutical application of the different nano-carriers with respect to the organ, tissue and cellular level pH environment.

show abstract

ε-Polylysine and next-generation dendrigraft poly-L-lysine: chemistry, activity, and applications in biopharmaceuticals

Cited by 63 publications

References 107 publications

Preparation and characterisation of novelty food preservatives by Maillard reaction between ε‐polylysine and reducing sugars

Preparation and characterisation of novelty food preservatives by Maillard reaction between ε‐polylysine and reducing sugars

Enhanced Prophylactic and Therapeutic Effects of Polylysine-Modified Ara h 2 DNA Vaccine in a Mouse Model of Peanut Allergy

pH-Responsive Polypeptide-Based Smart Nano-Carriers for Theranostic Applications

Contact Info

Product

Resources

About