ΔFosB regulates Ca2+ release and proliferation of goat mammary epithelial cells

Zheng, Heng; Li, Hui; Li, Lihui; Ma, Shaoyang; Liu, Xuemei

doi:10.1016/j.gene.2014.05.023

Cited by 10 publications

(10 citation statements)

References 29 publications

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“…Intracellular calcium trafficking of the cytoplasm and mitochondria were detect using Fluo-3 AM and Rhod-2 AM (2μM, Molecular probes, Life Technologies), respectively. Cells were treated as described previously [ 41 , 42 ], and screened by means of flow cytometry and use of a confocal laser scanning microscope (Leica, Solms, Germany).…”

Section: Methodsmentioning

confidence: 99%

Developmental transcriptome profiling of bovine muscle tissue reveals an abundant GosB that regulates myoblast proliferation and apoptosis

Wei

Yang

et al. 2017

Oncotarget

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The formation of bovine skeletal muscle involves complex developmental and physiological processes that play a vital role in determining the quality of beef; however, the regulatory mechanisms underlying differences in meat quality are largely unknown. We conducted transcriptome analysis of bovine muscle tissues to compare gene expression profiles between embryonic and adult stages. Total RNAs from skeletal muscle of Qinchuan cattle at fetal and adult stages were used to construct libraries for Illumina next-generation sequencing using the Ribo-Zero RNA sequencing (RNA-Seq) method. We found a total of 19,695 genes to be expressed in fetal and adult stages, whereby 3,299 were expressed only in fetal, and 433 only in adult tissues. We characterized the role of a candidate gene (GosB), which was highly (but differentially) expressed in embryonic and adult skeletal muscle tissue. GosB increased the number of myoblasts in the S-phase of the cell cycle, and decreased the proportion of cells in the G0/G1 phase. GosB promoted the proliferation of myoblasts and protected them from apoptosis via regulating Bcl-2 expression and controlling the intracellular calcium concentration. Modulation of GosB expression in muscle tissue may emerge as a potential target in breeding strategies attempting to alter myoblast numbers in cattle.

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Section: Methodsmentioning

confidence: 99%

Developmental transcriptome profiling of bovine muscle tissue reveals an abundant GosB that regulates myoblast proliferation and apoptosis

Wei

Yang

et al. 2017

Oncotarget

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“…Intracellular calcium trafficking of the cytoplasm and mitochondria were detected using Fluo-3 AM and Rhod-2 AM (2 μ M, Molecular probes, Life Technologies), respectively. Cells were treated as described previously [ 9 , 10 ] and screened by means of flow cytometry and the use of a confocal laser scanning microscope (Leica, Solms, Germany).…”

Section: Methodsmentioning

confidence: 99%

“…To better understand the molecular mechanisms behind the fat phenotype observed in GosB mice and to determine why adipocyte numbers are low in GosB mutants, we suspect that the proliferation and the apoptosis status of adipocyte could affect the mass of adipose tissue. It has been demonstrated that GosB not only is an important regulator of lipid synthesis but also has a role in cell survival in mammary cells [ 9 , 10 ], as data revealed that it can mediate cell proliferation and apoptosis [ 11 – 13 ]. Overexpression of GosB in goat mammary gland epithelial cells increased cell viability [ 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

“…It has been demonstrated that GosB not only is an important regulator of lipid synthesis but also has a role in cell survival in mammary cells [ 9 , 10 ], as data revealed that it can mediate cell proliferation and apoptosis [ 11 – 13 ]. Overexpression of GosB in goat mammary gland epithelial cells increased cell viability [ 9 , 10 ]. GosB protects rat embryo cells from apoptosis and exhibits a significantly higher survival occurring in the mitochondrial pathway dependent on caspase-3 and caspase-9 [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

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GosB Inhibits Triacylglycerol Synthesis and Promotes Cell Survival in Mouse Mammary Epithelial Cells

Xu¹,

Duan²,

Hou³

et al. 2017

BioMed Research International

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It has been demonstrated that the activator protein related transcription factor Finkel-Biskis-Jinkins murine osteosarcoma B (GosB) is involved in preadipocyte differentiation and triacylglycerol synthesis. However, the role of GosB in regulating the synthesis of milk fatty acid in mouse mammary glands remains unclear. This research uncovered potentially new roles of GosB in suppressing milk fatty acid synthesis. Results revealed that GosB had the highest expression in lung tissue and showed a higher expression level during nonlactation than during lactation. GosB inhibited the expression of fatty acid synthase (FASN), stearoyl-CoA desaturase (SCD), fatty acid binding protein 4 (FABP4), diacylglycerol acyltransferase 1 (DGAT1), perilipin 2 (PLIN2), perilipin 3 (PLIN3), and C/EBPα in mouse mammary gland epithelial cells (MEC). In addition, GosB reduced cellular triglyceride content and the accumulation of lipid droplets; in particular, GosB enhanced saturated fatty acid concentration (C16:0 and C18:0). The PPARγ agonist, rosiglitazone (ROSI), promoted apoptosis and inhibited cell proliferation. GosB increased the expression of Bcl-2 and protected MEC from ROSI-induced apoptosis. Furthermore, MECs were protected from apoptosis through the GosB regulation of intracellular calcium concentrations. These findings suggest that GosB may regulate mammary epithelial cells milk fat synthesis and apoptosis via PPARγ in mouse mammary glands.

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“…It is prominently expressed in the parathyroid and kidney where it regulates the secretion of parathyroid hormone (PTH) and the handling of calcium in the renal tubules, respectively [2]. During lactation, the mammary gland provides large amounts of calcium from the maternal circulation into the milk required for rapid skeletal growth during the neonatal period [3][4][5]. CaSR mRNA levels are low during post-natal mammary gland development through late pregnancy, but increase to a peak during lactation in the mouse mammary gland [6].…”

Section: Introductionmentioning

confidence: 99%

Parathyroid hormone-related protein overexpression protects goat mammary gland epithelial cells from calcium-sensing receptor activation-induced apoptosis

Sun

Zheng

et al. 2014

Mol Biol Rep

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Normal mammary gland epithelial cells and breast cancer cells express the calcium-sensing receptor (CaSR), which is the master regulator of systemic calcium metabolism. During lactation, activation of the CaSR in mammary epithelial cells downregulates parathyroid hormone-related protein (PTHrP) levels in milk and in the circulation, and increases calcium transport into milk. However, very little information is available on the role of CaSR in goat mammary gland epithelial cells (GMECs) apoptosis. In this investigation, the full-length cDNA of CaSR from Xinong Saanen dairy goats was cloned, which contains an open-reading frame of 3,258 bp encoding 1,085 amino acids with a predicted molecular weight of 121.0 kDa and an isoelectric point of 5.65. The amino acid sequence is highly homologous with sheep, and the goat CaSR gene is mapped to chromosome 1. Quantitative real-time PCR suggested that CaSR was predominantly expressed in the heart, kidney and mammary gland. Then, we found the stimulation of CaSR with its activator gadolinium chloride (GdCl3) contributed to increase CaSR mRNA levels in GMECs and simultaneously promoted cell apoptosis, and these effects were abrogated partially by NPS2390 which is an inhibitor of CaSR. We also demonstrated that Ca(2+) increased CaSR mRNA levels and induced GMECs apoptosis and restrained cell proliferation. In contrast, PTHrP overexpression protected GMECs from calcium-induced apoptosis, and promoted cell proliferation. In conclusion, these results suggest that PTHrP overexpression protects GMECs from CaSR activation-induced apoptosis.

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ΔFosB regulates Ca2+ release and proliferation of goat mammary epithelial cells

Cited by 10 publications

References 29 publications

Developmental transcriptome profiling of bovine muscle tissue reveals an abundant GosB that regulates myoblast proliferation and apoptosis

Developmental transcriptome profiling of bovine muscle tissue reveals an abundant GosB that regulates myoblast proliferation and apoptosis

GosB Inhibits Triacylglycerol Synthesis and Promotes Cell Survival in Mouse Mammary Epithelial Cells

Parathyroid hormone-related protein overexpression protects goat mammary gland epithelial cells from calcium-sensing receptor activation-induced apoptosis

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