δ-Integration of endo/exo-glucanase and β-glucosidase genes into the yeast chromosomes for direct conversion of cellulose to ethanol

Cho, Kwang Myung; Yoo, Young Je; Kang, Hyen Sam

doi:10.1016/s0141-0229(99)00011-3

Cited by 100 publications

(64 citation statements)

References 27 publications

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“…The final achieved ethanol yield by the recombinant strain was 2.3 g/l from 5 g/l cellobiose, compared to 2.1 g/l when glucose (5.26 g/l) was used as substrate. In another attempt, a recombinant strain of S. cerevisiae with β-glucosidase and an exo/endocellulase activity was produced, and as a result, external application of cellulase was significantly reduced (Cho et al, 1999). den Haan et al (2007a) produced a recombinant S. cerevisiae strain co-expressing the T. reesei EG1 (cel7B) and S. fibuligera β-glucosidase (cel3A) with an ability to grow on and convert 10 g/l cellulose to ethanol up to 1 g/l.…”

Section: -Expression Of Single or Many Hydrolase Genes In S Cerevisiaementioning

confidence: 99%

Advances in consolidated bioprocessing systems for bioethanol and butanol production from biomass: a comprehensive review

2015

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HIGHLIGHTS Various CBP strategies have been discussed. Recently, lignocellulosic biomass as the most abundant renewable resource has been widely considered for bioalcohols production. However, the complex structure of lignocelluloses requires a multi-step process which is costly and time consuming. Although, several bioprocessing approaches have been developed for pretreatment, saccharification and fermentation, bioalcohols production from lignocelluloses is still limited because of the economic infeasibility of these technologies. This cost constraint could be overcome by designing and constructing robust cellulolytic and bioalcohols producing microbes and by using them in a consolidated bioprocessing (CBP) system. This paper comprehensively reviews potentials, recent advances and challenges faced in CBP systems for efficient bioalcohols (ethanol and butanol) production from lignocellulosic and starchy biomass. The CBP strategies include using native single strains with cellulytic and alcohol production activities, microbial co-cultures containing both cellulytic and ethanologenic microorganisms, and genetic engineering of cellulytic microorganisms to be alcohol-producing or alcohol producing microorganisms to be cellulytic. Moreover, high-throughput techniques, such as metagenomics, metatranscriptomics, next generation sequencing and synthetic biology developed to explore novel microorganisms and powerful enzymes with high activity, thermostability and pH stability are also discussed. Currently, the CBP technology is in its infant stage, and ideal microorganisms and/or conditions at industrial scale are yet to be introduced. So, it is essential to bring into attention all barriers faced and take advantage of all the experiences gained to achieve a high-yield and low-cost CBP process.

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Section: -Expression Of Single or Many Hydrolase Genes In S Cerevisiaementioning

confidence: 99%

Advances in consolidated bioprocessing systems for bioethanol and butanol production from biomass: a comprehensive review

2015

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show abstract

“…Over the last thirteen years, a few studies representing significant progress towards the production of a cellulose-fermenting yeast strain were published. Cho and coworkers [11,12] reported an early example of a recombinant yeast strain that could functionally express several cellulases. Using δ-integration, they inserted multiple copies of two cellulase genes -encoding a bifunctional endo/exo-glucanase and a BGL-into the chromosomes of S. cerevisiae.…”

Section: Recombinant Cellulase Expression In Saccharomyces Cerevisiaementioning

confidence: 99%

Recombinant Cellulase and Cellulosome Systems

Wieczorek¹,

Biot-Pelletier²,

Martin³

2013

Cellulose - Biomass Conversion

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“…Endoglucanase genes from Bacillus spp. were successfully integrated (randomly, at approximately 44 sites) into the chromosome of yeast, resulting in the direct conversion of cellodextrin into ethanol (Cho et al 1999).…”

Section: S Cerevisiaementioning

confidence: 99%

Heterologous Expression and Extracellular Secretion of Cellulases in Recombinant Microbes

Parisutham¹,

Lee²

2012

Bioethanol

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δ-Integration of endo/exo-glucanase and β-glucosidase genes into the yeast chromosomes for direct conversion of cellulose to ethanol

Cited by 100 publications

References 27 publications

Advances in consolidated bioprocessing systems for bioethanol and butanol production from biomass: a comprehensive review

Advances in consolidated bioprocessing systems for bioethanol and butanol production from biomass: a comprehensive review

Recombinant Cellulase and Cellulosome Systems

Heterologous Expression and Extracellular Secretion of Cellulases in Recombinant Microbes

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