δ-Aminolevulinate dehydratase activity in type 2 diabetic patients and its association with lipid profile and oxidative stress

Bonfanti, Gabriela; Ceolin, Ronise B.; Valcorte, Tiago; Bona, Karine Santos De; Lucca, Leidiane de; Gonçalves, Thissiane L.; Moretto, Maria Beatriz

doi:10.1016/j.clinbiochem.2011.06.980

Cited by 17 publications

(17 citation statements)

References 44 publications

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“…Both diabetic groups in the current study exhibited highly significant increase of TC and highly significant decrease of HDL-C compared with the control group. However, only uncontrolled diabetics had significantly higher TG, LDL-C, TG/HDL-C, and LDL-C/HDL-C ratio when compared with the control and controlled DM groups, in agreement with Bonfanti et al [ 25 ]. These findings indicate dyslipidemia according to NCEP-ATP III [ 5 ] and could be attributed to OS [ 26 ], decreased activity of lipoprotein lipase and cholesterol ester transport protein [ 27 ], and increased free fatty acid mobilization from the liver [ 28 ].…”

Section: Discussionsupporting

confidence: 90%

Macrophage Migration Inhibitory Factor and Malondialdehyde as Potential Predictors of Vascular Risk Complications in Type 2 Diabetes Mellitus: Cross-Sectional Case Control Study in Saudi Arabia

Morsi

Ismail

Gaber

et al. 2016

Mediators of Inflammation

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Background. Malondialdehyde (MDA) has been implicated in the development of many acute inflammatory, autoimmune diseases as well as chronic inflammatory metabolic disorders. Involvement of inflammatory response and oxidative stress is currently suggested as a mechanism underlying development of diabetes and its complications. Objective. To evaluate the clinical utility of MDA, macrophage migration inhibitory factor (MIF), LDL-C/HDL-C, and TG/HDL-C ratio as noninvasive laboratory markers for prediction of T2DM vascular complications. Method. 63 Saudi T2DM patients and 16 age and sex matched controls were included. Serum MDA and MIF were assayed by thiobarbituric acid reactive substances and ELISA, respectively. TG/HDL-C and LDL-C/HDL-C ratios were calculated. Results. Uncontrolled DM patients had significantly higher levels of MDA, MIF, TG/HDL-C, and LDL-C/HDL-C ratios when compared with controlled DM patients and control group (p < 0.001). MDA had 100% sensitivity and 88% specificity. MIF showed 97% sensitivity and 100% specificity and LDL-C/HDL-C had 97% sensitivity and 95% specificity. Meanwhile, TG/HDL-C had the lowest sensitivity and specificity in identifying diabetic patients who would suffer from vascular complications. Conclusion. MDA, MIF, and LDL-C/HDL-C could be new predictors of metabolic disturbance which promote vascular complications in T2DM.

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Section: Discussionsupporting

confidence: 90%

Macrophage Migration Inhibitory Factor and Malondialdehyde as Potential Predictors of Vascular Risk Complications in Type 2 Diabetes Mellitus: Cross-Sectional Case Control Study in Saudi Arabia

Morsi

Ismail

Gaber

et al. 2016

Mediators of Inflammation

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“…A reduced activity of δ-ALA-D already was demonstrated in human and experimental diabetes (Bonfanti et al, 2011;Fernández-Cuartero et al, 1999;Souza et al, 2007) and the reactivation index of δ-ALA-D activity has been considered an interesting tool to estimate oxidative stress in chronic diseases (Gonçalves et al, 2009;Valentini et al, 2008). In fact, the reactivation index was increased only in T2DM/HT group when compared with controls (Table 2).…”

Section: Discussionmentioning

confidence: 88%

“…In line with this, the enzyme δ-aminolevulinate dehydratase (δ-ALA-D) is one of the most sensitive enzymes participating in heme biosynthesis, which catalyzes the synthesis of tetrapyrrolic compounds such as billins and hemes (Shemi, 1976;Tsukamoto, Yoshinaga, & Sano, 1979). Its activity already were found decreased in pathologies like cancer (Gonçalves et al, 2009), chronic renal failure (Fontanellas et al, 2002), and diabetes mellitus (Bonfanti et al, 2011;Fernández-Cuartero, Rebollar, Batlle, & Salamanca, 1999). Thus, this enzyme is extremely sensitive to situations associated with oxidative stress and might be altered in conditions like hypertension.…”

Section: Introductionmentioning

confidence: 94%

Hypertension strengthens δ-ALA-D activity inhibition and increases it reactivation index in type 2 diabetic patients

Bonfanti

Ceolin

Bona

et al. 2012

Journal of Diabetes and its Complications

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“…Os ratos foram eutanasiados por deslocamento cervical após anestesia inalatória, e o fígado e rim rapidamente dissecados, pesados e colocados em gelo. Os tecidos foram então homogeneizados em Tris-HCl, 10mM, pH 7,4 (1/10 p/v) e posteriormente centrifugados a 4.000rpm a 4°C por 10 minutos (BONFANTI et al, 2011).…”

Section: Preparo Dos Tecidos E Indução De Dano Por Paraquatunclassified

“…A determinação de grupamentos tiólicos não proteicos (NPSH) em tecidos hepático e renal foi realizada com a utilização do reagente de cor ácido 5',5' -ditio-bis-(2-nitrobenzoico) (DTNB); para a colorimetria e a leitura, foi realizada em espectrofotômetro em 412nm. Os dados foram expressos por μmol GSH/ mg proteína, baseados em uma curva de calibração com glutationa (GSH) (BOYNE; ELLMAN, 1972).…”

Section: Análise De Biomarcadores De Estresse Oxidativounclassified

Quercetina protege contra dano oxidativo causado por paraquat em fígado e rim de ratos

Hahn

Bortolotto

Claudino

et al. 2019

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As intoxicações por paraquat (Pq) são frequentes no país e seu mecanismo de ação tóxica envolve a geração de estresse oxidativo, que pode ser combatida com antioxidantes exógenos como a quercetina (Q). Assim, ao avaliar o papel desse flavonoide em dano causado por Pq em modelo experimental animal, in vitro, observou-se uma redução no nível de lipoperoxidação hepático, sugerindo maior sensibilidade desse tecido do que o renal. Também, quando em associação, Pq + Q foram capazes de provocar a inibição da enzima δ-aminolevulinato desidratase (δ-ALA-D), possivelmente por mecanismo de quelação, não relacionado aos grupos sulfidrílicos da enzima. In silico, a Q apresentou boa absorção intestinal, alta taxa de ligação às proteínas plasmáticas, potencial mutagênico, carcinogênico e inibitório sobre enzimas do metabolismo. Assim, acredita-se que a dieta alimentar rica em Q ou sua suplementação pode trazer benefícios à saúde da população, especialmente àqueles expostos a herbicidas, como o Pq.

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δ-Aminolevulinate dehydratase activity in type 2 diabetic patients and its association with lipid profile and oxidative stress

Cited by 17 publications

References 44 publications

Macrophage Migration Inhibitory Factor and Malondialdehyde as Potential Predictors of Vascular Risk Complications in Type 2 Diabetes Mellitus: Cross-Sectional Case Control Study in Saudi Arabia

Macrophage Migration Inhibitory Factor and Malondialdehyde as Potential Predictors of Vascular Risk Complications in Type 2 Diabetes Mellitus: Cross-Sectional Case Control Study in Saudi Arabia

Hypertension strengthens δ-ALA-D activity inhibition and increases it reactivation index in type 2 diabetic patients

Quercetina protege contra dano oxidativo causado por paraquat em fígado e rim de ratos

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