Δ-9-Tetrahydrocannabinol Analysis in Forensic Blood Specimens Using Capillary Column Gas Chromatography with Nitrogen Sensitive Detection

Abstract

A method for the determination of delta-9-tetrahydrocannabinol (THC) in whole blood that employs hexane extraction followed by identification using gas chromatography with nitrogen/phosphorus detection has been developed. The assay is sensitive to 2 ng THC per mL blood and linear to concentrations up to 120 ng/mL (r = 0.998). Precision is demonstrated by coefficients of variation ranging from 3.6 to 9.1% (within run) and 7.1 to 8.0% (between run).

Water analysis

“…A method for the determination of A9-THC in whole blood that employs hexane extraction followed by identification using GC-NPD has been developed which is sensitive to 2 ng of THC/mL of blood (71). The sensitivity and specificity of serum A9-THC and ll-nor-9-carboxy-A9-THC by RIA in identifying habitual daily smokers of marijuana have been reported (72).…”