γδTFH cells promote B cell maturation and antibody production in neuroblastoma

Abstract

BackgroundPrevious studies have shown that γδ TFH cells are capable of modulating antibody production in immunized and infected mouse model. In recent studies, human γδ TFH cells are shown to contribute to the activation of humoral immunity and promote the maturation of B cells. However, little information is available on their involvement in neuroblastoma (NB) pathogenesis.ResultsIn the present study, the frequency of γδ TFH cells in 74 NB patients was significantly higher compared with that in 60 healthy controls. Moreover, most γδ TFH cells in NB patients had a naive phenotype with up-regulation of CD25, CD69, HLA-DR and CD40L and down-regulation of ICOS. Importantly, γδ TFH cells in NB patients produced more IL-4 and IL-10 than those in healthy controls. Furthermore, serum total IgG level was significantly increased in NB patients compared with healthy controls. The expression of CD23 on B cells was up-regulated while CD80 expression was significantly down-regulated in NB patients. Further analysis of B cell compartment showed that the frequency of CD19+CD27hi plasma cells was enhanced in NB patients. Spearman’s correlation analysis revealed that the frequency of γδ TFH cells was positively correlated to serum total IgG level and CD19+CD27hi plasma cells in NB patients, but negatively correlated to CD19+ B cells.ConclusionsWe concluded that γδ TFH cells might promote B cell maturation and antibody production in NB patients.

Identification of an Autophagy-Related Risk Signature Correlates With Immunophenotype and Predicts Immune Checkpoint Blockade Efficacy of Neuroblastoma

“…Besides, B lymphocytes and T follicular helper cells also produce a marked effect in the microenvironment. Wenjun et al reported that the subtype γδTFH cells were positively correlated with serum total immunoglobulin G and plasma cells in NB patients, and all the components mentioned earlier were significantly higher than in normal children (Mou et al, 2017). Last but not least, M2 macrophage was reported to be regulated by the mTOR pathway, an important autophagy-related pathway (Chen et al, 2020).…”

Identification of a Five-Gene Signature Derived From MYCN Amplification and Establishment of a Nomogram for Predicting the Prognosis of Neuroblastoma

“…TFh plays an important role in humoral immunity ( Eivazi et al, 2016 ). A previous study in NB showed that γδ TFh cells might facilitate the maturation of B cells and the production of antibodies ( Mou et al, 2017 ). In studies of colorectal and breast cancer, infiltrating TFh cells have also been found to be positively correlated with prognosis ( Bindea et al, 2013 ; Gu-Trantien et al, 2013 ).…”

Research progress of Chinese herbal medicines and classic prescriptions in the treatment of COPD based on the TCM theory and medicinal characteristics

“…By upregulating the protein and gene levels of GATA-3 and downregulating the protein and gene levels of T-bet in the lung tissue, the modified decoction could treat lung tissue inflammation in COPD rats and reduce the immune disorder. [146,147] Moreover, it can promote the release of IL-12 and IFN-γ by Th1 cells and inhibit the expression of IL-4, IL-13 and their receptors IL-4RA1/IL-13RA1, thus increasing IL-12 and IFN-γ and reducing IL-9, IL-4, and IL-13. The modified decoction shows protective effects on the structure of bronchioles, thus protecting the lung function in COPD.…”