γH2AX signalling during sperm chromatin remodelling in the mouse zygote

Derijck, Alwin A.H.A.; Heijden, Godfried W. van der; Giele, Maud; Philippens, M.E.P.; Bavel, C.C.A.W. van; Boer, P. de

doi:10.1016/j.dnarep.2006.05.043

Cited by 48 publications

(35 citation statements)

References 62 publications

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“…Our recent data show that radiation-induced DSBs in the sperm head are repaired in the wild-type zygote during pre S phase (Derijck et al, 2006), where they should be almost exclusively repaired by the NHEJ pathway (Rothkamm et al, 2003). Given that unrepaired DSBs are highly mutagenic and result in chromosome type aberrations in early zygote (Matsuda and Tobari, 1989), their early repair in the offspring of #BALB/c Â~scid mating should be compensated, most likely by homologous recombination, HR (Allen et al, 2002).…”

Section: Maternal Effects and Genetic Instability T Hatch Et Almentioning

confidence: 99%

“…Given that the efficiency of DNA repair in sperm cell is greatly diminished (Olsen et al, 2005) and the fertilizing ability of irradiated sperm is not substantially compromised (Ahmadi and Ng, 1999), the majority of premutational radiation-induced lesions are transmitted to the fertilized egg, where they are repaired within a few hours (Matsuda and Tobari, 1989;Derijck et al, 2006). As a result, radiation-induced damage to sperm DNA triggers a cascade of events in the zygote, including profound changes in the expression of DNA repair genes (Harrouk et al, 2000, Shimura et al, 2002) and alterations in DNA methylation and histone acetylation (Barton et al, 2005).…”

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confidence: 99%

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Maternal effects of the scid mutation on radiation-induced transgenerational instability in mice

et al. 2007

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The results of a number of recent studies show that mutation rates in the offspring of irradiated parents are substantially elevated, however, the effect of parental genotype on transgenerational instability remains poorly understood. Here, we have analysed the mutation frequency at an expanded simple tandem repeat (ESTR) locus in the germline and bone marrow of the firstgeneration male offspring of control and irradiated male mice. The frequency of ESTR mutation was studied in the offspring of two reciprocal matings #scid Â~BALB/c and #BALB/c Â~scid, which were compared with that in BALB/c mice. In the offspring of the BALB/c Â BALB/c and #scid Â~BALB/c matings, which were conceived after paternal sperm irradiation, the frequency of ESTR mutation was significantly elevated in both tissues. In contrast, ESTR mutation frequency was only slightly elevated in the offspring of #BALB/c Â~scid mating conceived after paternal irradiation. The results of this study suggest that the oocytes of scid females are unable to fully support the repair of double-strand breaks induced in paternal sperm which may in turn result in the elimination of cells/embryos containing high levels of DNA damage, thus partially preventing the manifestation of genomic instability.

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Section: Maternal Effects and Genetic Instability T Hatch Et Almentioning

confidence: 99%

mentioning

confidence: 99%

Maternal effects of the scid mutation on radiation-induced transgenerational instability in mice

et al. 2007

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“…After phosphorylation of H2AX triggered by DSB, g-H2AX at the DSB recruits numerous DNA repair proteins, including RAD51, BRCA1 and 53BP1 [Fernandez-Capetillo et al 2004;Li et al 2005]. Therefore, g-H2AX is essential for DNA repair and maintaining genomic stability [FernandezCapetillo et al 2004;Li et al 2005] and is involved in sperm chromatin remodeling after fertilization [Derijck et al 2006]. H2AX is one of the evolutionarily conserved fertility factors as determined by sperm chromatin proteomics, and is considered a candidate gene of human male infertility [Chu et al 2006].…”

Section: Introductionmentioning

confidence: 99%

Absence of theH2AXMutations in Idiopathic Infertile Men with Spermatogenic Impairment

Zhang

Yang

et al. 2008

Systems Biology in Reproductive Medicine

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H2AX is a histone H2A variant and one of the evolutionarily conserved fertility factors involved in DNA repair to maintain the genomic integrity and ensure the proper meiotic process. Male H2ax mutant mice are infertile and display defective meiosis. To investigate the possible association of variations of the H2AX gene with spermatogenic impairment in humans, mutation screening of the entire coding region of this gene was carried out in 302 patients with azoospermia or severe oligospermia along with 198 normospermic controls. No mutations or other sequence variants were identified in the 500 subjects tested. This suggests that it is unlikely that the H2AX mutations are a common genetic cause of spermatogenic impairment in idiopathic infertile men.

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“…Después de la fertilización, el genoma masculino se somete a una descondensación y es reempaquetado con histonas presentes en el citoplasma del ovocito (75) . Una vez que ambos genomas haploides se encuentran empaquetados con histonas, comienza la replicación del DNA de forma independiente en cada pronúcleo.…”

Section: Efecto Materno Sobre La Epigénesisunclassified

“…After fertilization, the male genome undergoes a decondensation and is repackaged with histones present in the cytoplasm of the oocyte (75) . Once both haploid genomes are packaged with histones, the DNA replication begins independently in each pronucleus.…”

Section: Efecto Materno Sobre La Epigénesismentioning

confidence: 99%

Efectos de los RNAm maternos sobre la maduración del ovocito y el desarrollo embrionario temprano en mamíferos

Burrola-Barraza¹,

González-Rodríguez

2015

Rev. Mex. Cienc. Pecu.

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Efectos de los RNAm maternos sobre la maduración del ovocito y el desarrollo embrionario temprano en mamíferos. RevisiónEffects of the maternal mRNA on the maturation of the oocyte and early embryonic development in mammals. Review M. Eduviges Burrola-Barraza a , Everardo González-Rodríguez a RESUMENObtener ovocitos madurados in vitro, que sean competentes para fertilizarse y originar un porcentaje superior al 50 % de blastocitos viables, es una de las principales metas que existen en el desarrollo in vitro de embriones bovinos. Es por ello necesario entender los procesos celulares, que desembocan en la maduración del ovocito durante la foliculogénesis y su subsecuente transición al embrión. La transición del ovocito al embrión es un proceso complejo que involucra la inactivación genómica del ovocito y la activación del genoma embrionario. Este proceso se da en bovinos en la etapa de 8 a 16 células y presenta una degradación selectiva de RNAm maternos, que fueron almacenados durante la ovogénesis y son la fuente para la codificación de proteínas en las etapas iníciales del desarrollo embrionario. La acción de los RNAm maternos es clave para que la activación del genoma embrionario se realice en tiempo y forma adecuados. Entender la participación de estos transcritos en la activación del genoma embrionario, es esencial para esclarecer los procesos celulares que fallan en los protocolos de maduración de ovocitos in vitro. Es por esto que el objetivo de esta revisión fue recopilar la información de los principales RNAm maternos que han sido identificados tanto en el modelo murino como el bovino, esto con el fin de integrar el conocimiento relacionado con los procesos genómicos que permiten el desarrollo del embrión en el bovino, y diseñar nuevas estrategias que permitan mejorar los protocolos de fertilización in vitro.PALABRAS CLAVE: Ovocito, Activación genoma embrionario, Bovino. ABSTRACTGet in vitro matured oocytes that could be competent for in vitro fertilization with a result of 50 % viable blastocysts, is one of the main problems in the in vitro development of bovine embryos. For these reason, is necessary to understand the cellular processes that lead to the maturation of the oocyte during folliculogenesis and subsequent transition to the embryo. The transition from oocyte to embryo is a complex process that involves genomic inactivation of the oocyte and embryonic genome activation. This process occurs in cattle in the stage of 8 to 16 cells where there are a selective degradation of maternal mRNA, which were stored during oogenesis and are the source of protein in the early stages of embryonic development. The action of maternal mRNA is a key for that embryonic genome activation takes place in form and time. Understanding the involvement of these transcripts in embryonic genome activation is essential to elucidate the cellular processes falling in oocyte maturation in vitro protocols. The aim of this review was to gather information from maternal genes that have been isolated both in the murine model and c...

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γH2AX signalling during sperm chromatin remodelling in the mouse zygote

Cited by 48 publications

References 62 publications

Maternal effects of the scid mutation on radiation-induced transgenerational instability in mice

Maternal effects of the scid mutation on radiation-induced transgenerational instability in mice

Absence of theH2AXMutations in Idiopathic Infertile Men with Spermatogenic Impairment

Efectos de los RNAm maternos sobre la maduración del ovocito y el desarrollo embrionario temprano en mamíferos

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