γ-Oryzanol suppresses cell apoptosis by inhibiting reactive oxygen species-mediated mitochondrial signaling pathway in H2O2-stimulated L02 cells

Huang, Lin; Jiang, Weiwei; Zhu, Lijuan; Ma, Chaoyang; Ou, Zhaoping; Luo, Chenxi; Wu, Jing; Wen, Lixin; Tan, Zhuliang; Yi, Jine

doi:10.1016/j.biopha.2019.109554

Cited by 34 publications

(39 citation statements)

References 37 publications

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“…In this study, we showed that the EtOAc fraction significantly increased the cell viability, reduced LDH release, It is well-known that these critical antioxidant enzymes play a major role in ROS scavenging [26]. As already mentioned, extracts of D. moldavica L. could reduce the MDA level and increase the contents of SOD and CAT in diabetic rats [27].…”

Section: Discussionsupporting

confidence: 64%

Dracocephalum moldavica L. Extracts Protect H9c2 Cardiomyocytes against H₂O₂-Induced Apoptosis and Oxidative Stress

Jin

et al. 2020

BioMed Research International

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Background and Objectives. Dracocephalum moldavica L. is an herbal medicine used to treat cardiovascular diseases in China. The purpose of this study was to assess the cardioprotective effect of D. moldavica L. extracts against H2O2-induced apoptosis and oxidative stress in H9c2 cells and to explore the mechanism behind this effect. Materials and Methods. The petroleum ether (petrol), dichloromethane (CH2Cl2), ethyl acetate (EtOAc), and n-butyl alcohol (n-BuOH) fractions were isolated from alcohol extracts of D. moldavica L. Total phenolic and flavonoid contents and in vitro antioxidant activities of different fractions were evaluated. H9c2 cells were then treated with D. moldavica L. extracts before challenging with H2O2. Cell viability was determined by colorimetric assay, and ELISA was used to measure the levels of lactate dehydrogenase (LDH), malondialdehyde (MDA), and superoxide dismutase (SOD). Apoptosis levels and mitochondrial membrane potential were measured by flow cytometry. The expressions of cell apoptosis regulatory proteins caspase-3, Bax, and Bcl-2 were determined by western blotting. Results. Our results demonstrated that the EtOAc fraction from D. moldavica L. ethanol extract, which is rich in phenolic and flavonoid active constituents, had the strongest free radical scavenging activity. Additionally, this fraction increased H2O2-induced reduction in cell viability, SOD activity, and mitochondrial membrane potential. It also reduced H2O2-induced elevation in ROS production, contents of LDH and MDA, and H9c2 apoptosis. We further found that the EtOAc fraction increased Bcl-2 expression, while it decreased caspase-3 and Bax expressions induced by H2O2 in H9c2 cells. Conclusions. Our data revealed that the EtOAc fraction from D. moldavica L. ethanol extract ameliorates H2O2-induced cardiotoxicity via antiapoptotic and antioxidant mechanisms.

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Section: Discussionsupporting

confidence: 64%

Dracocephalum moldavica L. Extracts Protect H9c2 Cardiomyocytes against H₂O₂-Induced Apoptosis and Oxidative Stress

Jin

et al. 2020

BioMed Research International

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“…20 GO could suppress ROS accumulation and ROS-activated mitochondrial apoptotic pathway in a hepatic cell line. 19 However, the impact of GO on renal IRI-induced distant organ dysfunction has not been reported to our knowledge. In this study, the results pointed out the potential of GO to ameliorate extrarenal organ dysfunctions after IRI in vivo .…”

Section: Discussionmentioning

confidence: 88%

“…The anti-inflammatory and antioxidant effects of GO are reported in different studies. [17][18][19][20][21] Understanding the complex connections between renal and distant organs can result in the development of novel diagnostics and therapeutic strategies to recover outcomes in cases with AKI. In the present study, the effects of GO on I/R-induced oxidative stress in distant organs including brain, heart, and liver of rats were evaluated.…”

Section: Introductionmentioning

confidence: 99%

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Protective effects of Gamma Oryzanol on distant organs after kidney ischemia-reperfusion in rats: A focus on liver protection

et al. 2020

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Background: Acute kidney injury (AKI) is the main clinical concern resulted from ischemia-reperfusion injury (IRI). Ample clinical data indicates that AKI is associated with distant organ dysfunctions and poor patients’ outcomes. Oxidative stress and inflammation have a critical role in the pathogenesis of organ injuries following IRI. The objectives of this study were to determine the impact of Gamma Oryzanol (GO), extracted from rice bran oil, on distant organs in rats after IRI. Methods: Twelve out of 24 Wistar rats were treated by one dosage of GO (100mg/kg) 1 h before I/R induction through both oral gavage and intraperitoneal injection. Then, the AKI model rats were induced by IRI. Oxidative stress and antioxidant protein levels were assessed in the brain, heart, and liver tissues in the experimental groups. Furthermore, the effects of GO on IRI-induced liver dysfunction, apoptosis, and inflammation were measured by Western blot. Results: GO pretreatment could significantly restore the levels and activity of antioxidant proteins in the brain, heart, and liver tissues (P < 0.05). Moreover, GO pretreatment could decrease the inflammatory cytokine (IL-1, IL-6, and TNF-α) in the liver (P < 0.01). By reducing Bax/Bcl-2 ratio and down-regulating caspase-3, GO could significantly diminish apoptosis in the liver tissue after the kidney I/R (P < 0.01). Additionally, GO could significantly diminish the deterioration of liver function in the kidney I/R model. Conclusion: GO protects distant organs against renal IRI-induced oxidative stress. Furthermore, it ameliorates liver function and remarkably exerts anti-oxidative, anti-inflammatory, and anti-apoptotic roles in the liver as an important detoxifying organ.

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“…The L02 cells were purchased from BeNa Culture Collection Company (BNCC, Beijing, China) and cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin solution at 37 °C under 5% CO 2 in a humidified incubator. The H 2 O 2 induced oxidative stress model of L02 cell was established according to the reported method with slight modifications [ 28 , 29 , 30 ]. Firstly, 120 µL L02 cell suspension (1 × 10 4 cells) was seeded in a 96-well plate and incubated for 24 h, followed by the addition of 15 µL PBS buffer (corresponding to the addition of tested samples).…”

Section: Methodsmentioning

confidence: 99%

Phenolic Compounds from Carissa spinarum Are Characterized by Their Antioxidant, Anti-Inflammatory and Hepatoprotective Activities

et al. 2021

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Carissa spinarum has been traditionally used for the treatment of various diseases due to its different pharmacological activities. However, the active compounds responsible for its potentially specific activities have rarely been explored. To this end, the ethyl acetate (EA) fraction was screened out and selected for further phytochemical isolation because of its promising activities in preliminary 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP) and COX-2 inhibition assays. As a result, 10 compounds (1−10), including a new one (5), were isolated, with eight of these being identified as phenolic compounds, as expected. Compound 9 possessed an IC50 value of 16.5 ± 1.2 µM, which was lower than that of positive control (vitamin C, 25.5 ± 0.3 µM) in the DPPH assay, and compounds 2, 6, 7 and 9 showed better total antioxidant capacity than vitamin C in the FRAP assay. Meanwhile, compounds 1−6 and 9 also had IC50 values of less than 1.0 µM, which was even better than the positive control indomethacin in the COX-2 inhibition assay. In this context, compounds 2 and 9 were further evaluated to exhibit clear hepatoprotective activities by improving the L02 cell viability and reducing ROS production using a H2O2-induced L02 cell injury model. This study provides initial evidence revealing the most potent phenolic compounds from the root bark of C. spinarum responsible for its antioxidant, anti-inflammatory and hepatoprotective activities.

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γ-Oryzanol suppresses cell apoptosis by inhibiting reactive oxygen species-mediated mitochondrial signaling pathway in H2O2-stimulated L02 cells

Cited by 34 publications

References 37 publications

Dracocephalum moldavica L. Extracts Protect H9c2 Cardiomyocytes against H₂O₂-Induced Apoptosis and Oxidative Stress

Dracocephalum moldavica L. Extracts Protect H9c2 Cardiomyocytes against H₂O₂-Induced Apoptosis and Oxidative Stress

Protective effects of Gamma Oryzanol on distant organs after kidney ischemia-reperfusion in rats: A focus on liver protection

Phenolic Compounds from Carissa spinarum Are Characterized by Their Antioxidant, Anti-Inflammatory and Hepatoprotective Activities

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γ-Oryzanol suppresses cell apoptosis by inhibiting reactive oxygen species-mediated mitochondrial signaling pathway in H2O2-stimulated L02 cells

Cited by 34 publications

References 37 publications

Dracocephalum moldavica L. Extracts Protect H9c2 Cardiomyocytes against H2O2-Induced Apoptosis and Oxidative Stress

Dracocephalum moldavica L. Extracts Protect H9c2 Cardiomyocytes against H2O2-Induced Apoptosis and Oxidative Stress

Protective effects of Gamma Oryzanol on distant organs after kidney ischemia-reperfusion in rats: A focus on liver protection

Phenolic Compounds from Carissa spinarum Are Characterized by Their Antioxidant, Anti-Inflammatory and Hepatoprotective Activities

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Dracocephalum moldavica L. Extracts Protect H9c2 Cardiomyocytes against H₂O₂-Induced Apoptosis and Oxidative Stress

Dracocephalum moldavica L. Extracts Protect H9c2 Cardiomyocytes against H₂O₂-Induced Apoptosis and Oxidative Stress