γ Irradiation and cellular damage in Kocuria rosea: investigation by one- and two-dimensional infrared spectroscopy

Melin, Anne-Marie; Perromat, Annie; Lorin, Chrystelle; Déléris, Gérard

doi:10.1016/s0003-9861(02)00583-0

Cited by 13 publications

(7 citation statements)

References 40 publications

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“…M. luteus and Kocuria spp. are known for their UV resistance [32,33], but this study confirms that Chryseobacterium spp. isolated from the coastal regions can also tolerate UV exposure.…”

Section: Resultssupporting

confidence: 53%

Evaluation of Antioxidant Potential and UV Protective Properties of Four Bacterial Pigments

Koshti

Jagtap

Noronha

et al. 2022

Microbiol. Biotechnol. Lett.

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In the present study, four distinctly colored bacterial isolates that show intense pigmentation upon brief ultraviolet (UV) light exposure are chosen. The strains are identified as Micrococcus luteus (Milky yellow), Cryseobacterium pallidum (Yellow), Cryseobacterium spp. (Golden yellow), and Kocuria turfanensis (Pink) based on their morphological and 16S rDNA analysis. Moderate salinity (1.25%), 25-37℃ temperature, and pH of 7.2 are found to be the most favorable conditions of growth and pigment production for all the selected isolates. The pigments are extracted using methanol: chloroform (1:1) and the purity of the pigments are confirmed by high-performance liquid chromatography (HPLC) and thin-layer chromatography (TLC). Further, Fourier transform infrared (FTIR) and UV-Visible spectroscopy indicate their resemblance with carotenoids and flexirubin family. The antioxidant activities of the pigments are estimated, and, all the pigments have shown significant antioxidant efficacy in 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-picryl-hydrazyl (DPPH), and ferric reducing antioxidant power (FRAP) assays. The UV protective property of the pigments is determined by cling-film assay, wherein, at least 25% of UV sensitive Escherichia coli survive with bio-pigments even after 90 seconds of UV exposure compared to control. The pigments also hold a good sun protective factor (SPF) value (1.5-4.9) which is calculated with the Mansur equation. Based on these results, it can be predicted that these bacterial pigments can be further developed into a promising antioxidant and UV-protectant for several biomedical applications.

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“…M. luteus and Kocuria spp. are known for their UV resistance [32,33], but this study confirms that Chryseobacterium spp. isolated from the coastal regions can also tolerate UV exposure.…”

Section: Resultssupporting

confidence: 53%

Evaluation of Antioxidant Potential and UV Protective Properties of Four Bacterial Pigments

Koshti

Jagtap

Noronha

et al. 2022

Microbiol. Biotechnol. Lett.

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“…Melin et al [193] reported the 2D IR study of time evolution and dose response of cellular membrane damage by g-ray of bacterium Kocuria resea. Evolutionary time-dose response was analyzed in this study.…”

Section: Other Perturbation Methodsmentioning

confidence: 99%

“…2D IR of bacterioRhodopsin (bR) under cyclic photo-induced perturbation was reported by Kosters et al [223]. Melin et al [193] used 2D IR to study time evolution and dose response of cellular membrane damage by g-ray of bacterium K. resea. 2D IR of UV-induced photochemical photochromic reaction process of Daetome conformational transformation was also reported by Wang et al [194].…”

Section: Photoinduced Processmentioning

confidence: 99%

Advances in two-dimensional correlation spectroscopy

Noda

2004

Vibrational Spectroscopy

235

149

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“…These investigations have since evolved to Raman spectroscopic studies of the effects of in-vitro proton irradiation of excised tissues [52], and radiotherapeutically irradiated (γ-radiation) tissue specimens [53][54][55]. Other studies with bacteria have investigated the effects of γ-radiation on Deinococcus radiodurans [56], Kocuria rosea [57]and Micrococcus luteus [58] with FTIR spectroscopy. Recently Gault et al have demonstrated the sensitivity of FTIR spectroscopy to apoptotic effects in γ-irradiated lymphocytes [59] and to molecular changes in HaCaT cells exposed to both γ-radiation and hydrogen peroxide [60], together with its sensitivity to biochemical alterations in HaCaT cells irradiated with α-particles delivered by micro-beams [61].…”

Section: Introductionmentioning

confidence: 99%

Spectroscopic and chemometric approaches to radiobiological analyses

Meade

Byrne²,

Lyng

2010

Mutation Research/Reviews in Mutation Research

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Vibrational spectroscopy is an attractive modality for the analysis of biological samples, providing a complete non-invasive acquisition of the biochemical fingerprint of the sample. It has been demonstrated that this data provides the means to assay multiple functional responses of a biological system at a spatial resolution as low as a micron within the sample. As the interaction of ionizing radiation with biological systems involves chemical reactions between the products of radiation induced damage and various structural and functional units within the cell, the vibrational spectroscopic modalities have received attention as potential measurement platforms for the in-situ examination of the chemistry of biological species in radiobiology. This presents challenges in relation to sample preparation and the construction of suitable analytical methodologies. In this work protocols for sample preparation and approaches to multivariate analysis of vibrational spectra in radiobiological analysis are detailed and the utility of the methodology in analyzing the evolution of biochemical responses to radiobiological damage are highlighted.

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γ Irradiation and cellular damage in Kocuria rosea: investigation by one- and two-dimensional infrared spectroscopy

Cited by 13 publications

References 40 publications

Evaluation of Antioxidant Potential and UV Protective Properties of Four Bacterial Pigments

Evaluation of Antioxidant Potential and UV Protective Properties of Four Bacterial Pigments

Advances in two-dimensional correlation spectroscopy

Spectroscopic and chemometric approaches to radiobiological analyses

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