γ‑irradiated prednisolone promotes apoptosis of liver cancer cells via activation of intrinsic apoptosis signaling pathway

Abstract

Prednisolone is an anti-inflammatory drug used to treat a number of conditions, including liver disease and cancer. Numerous studies have demonstrated that glucocorticoids such as prednisolone modified by ionizing radiation can promote anticancer activity in cancer cells. To the best of our knowledge, however, the effect of ionizing radiation on prednisolone structure and cancer cells has not yet been identified. The present study created a novel prednisolone derivative using γ-irradiation, and its anticancer properties were investigated in liver cancer cells. The present study confirmed the structure of the new prednisolone derivative using liquid chromatogram-mass spectrometry. MTT assays determined the cytotoxic effects of γ-irradiated (IR)-prednisolone in liver cancer cells. Flow cytometry analysis evaluated apoptosis, mitochondrial membrane potential and cell cycle distribution. Western blotting was used to analyze the proteins associated with apoptosis. The chromatogram profile revealed that IR-prednisolone produced a number of peaks compared with the single peak of the original prednisolone. In contrast to prednisolone, the MTT results showed that IR-prednisolone significantly prevented the growth of liver cancer cells. IR-prednisolone promoted apoptosis and arrested the cell cycle at the G0/G1 stage in Huh7 cells. IR-prednisolone also altered the mitochondrial membrane potential and activated caspase-associated proteins, which activated the intrinsic apoptotic signaling pathway. In conclusion, IR-prednisolone promoted anticancer effects in liver cancer cells via apoptosis activation. The present study demonstrated that IR-prednisolone may be a potential anticancer agent against liver cancer, although specific molecules have yet to be identified.

Novel aminopyridazine derivative of minaprine modified by radiolysis presents potent anti-inflammatory effects in LPS-stimulated RAW 264.7 and DH82 macrophage cells

“…In a recent study, chrysin and luteolin showed improved anti-in ammatory activities in vitro and in vivo induced by γ-irradiation, and the chemical structure of hydroxyalkyl-substituted compounds was identi ed 12,21 . In addition, radiolytic transformation of alkaloids and steroids showed potent enhanced anti-cancer effects in melanoma, breast, liver, and lung cancer 11,[22][23][24][25] .…”

Prednisolone and mesenchymal stem cell preloading protect liver cell migration and mitigate extracellular matrix modification in transplanted decellularized rat liver

“…While the cell viability in the presence of prednisolone and 2D condition was similar to control culture, it seems the releasing pattern of prednisolone is different in the alginate and decellularized scaffold. Prednisolone has also an anti-tumor effect, diminishes cell proliferation, and induces apoptosis in liver cancer cells such as HepG2 [ 61 ].…”

Big Data Analysis and Application of Liver Cancer Gene Sequence Based on Second-Generation Sequencing Technology

“…In the follow-up continuous improvement, the Sanger method caused a sequencing boom and became the mainstream due to its simplicity and rapidity. In order to meet the increasingly complex research needs, next-generation sequencing technology emerges from time to time [1][2][3]. Using NGS technology to detect a variety of cancers, and compared the results with the Sanger method, it was found that in addition to common gene mutations, NGS technology can also detect many gene mutations that were ignored by real-time quantitative PCR detection technology.…”