γ-Glutamyltranspeptidase and pH based “AND” logic gate fluorescent probe for orthotopic breast tumor imaging

Li, Kun; Zhang, Lina; Zhang, Hong; Chen, Shanyong; Liu, Yan-Zhao; Yang, Xiaohua; Xiang, Fei-Fan; Liu, Yanhong; Yu, Xiao‐Qi

doi:10.1039/d2cc06568a

Cited by 11 publications

(5 citation statements)

References 27 publications

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“…At the cell level, we carry out studies. Based on reported experience, these three amino-substituted probes can target lysosomes, , so we used HepG2 to explore their ability of subcellular localization properties by confocal microscopy. Initially, the cytotoxic properties of the three probes on HepG2 cells were investigated using the CCK-8 assays method through the incubation of different concentrations of these from 0 to 10 μM for 24 h (Figure S11 in the Supporting Information).…”

Section: Resultsmentioning

confidence: 99%

Rational Design of Near-Infrared Fluorescent Probes for Accurately Tracking Lysosomal Viscosity with Allyl Snchoring Si-Rhodamine

Xiang

Zhou

et al. 2023

Chemical & Biomedical Imaging

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The abnormal microenvironment parameter, viscosity, is closely connected with various diffusion processes, signal transduction, molecule interactions, and various diseases. It is greatly significant to design viscosity-dependent near-infrared (NIR) small molecule fluorescence probes for visualizing biological processes or diagnosing diseases. Herein, through the stepwise modulating structure of the silicon−rhodamine fluorophore (SR), we report three viscosity probes with allyl or methyl group as rotors, named SR-T-Al, SR-S-Al, and SR-T-Me. Among them, SR-T-Al demonstrates better viscosity responsibility from 1.0 to 1410.4 cP of viscosity. Therefore, the probe of SR-T-Al is successfully applied to sensitively monitor lysosome microscopic viscosity changes of living cells induced by oxygen stress. What's more, based on its advantages in NIR emission (669 nm) and large Stokes shift (201 nm), we also use it to image variations of viscosity in an acute hepatitis mouse induced by carbon tetrachloride. Both time and concentration-dependent induction models display the great ability of SR-T-Al to detect viscosity alteration. All the experimental results indicated that this allyl-rotor-based NIR viscosity probe could provide a general platform to monitor abnormal physiological processes and diseases relating to viscosity.

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Section: Resultsmentioning

confidence: 99%

Rational Design of Near-Infrared Fluorescent Probes for Accurately Tracking Lysosomal Viscosity with Allyl Snchoring Si-Rhodamine

Xiang

Zhou

et al. 2023

Chemical & Biomedical Imaging

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“…Meanwhile, Yu and colleagues developed a new NIR fluorescent probe in 2023 ( 13 , Si-NH 2 -Glu) that can be activated in the presence of both GGT and an acid environment in vivo . 39 The probe is comprised of the NIR meso-amine Si–rhodamine as a fluorophore and glutamic acid as a recognition unit for GGT. In the process of probe identification, only in the presence of both GGT and H + is the fluorescence signal at 543 nm of the probe released completely.…”

Section: The Design and Application Of Small-molecule Double-locked L...mentioning

confidence: 99%

Recent progress in the development of small-molecule double-locked logic gate fluorescence probes

Zhang,

Xiu,

Wang

et al. 2023

Chem. Commun.

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“…Another example of a meso-amine Si-rhodamine fluorophore-based responsive probe for GGT measurement represented an interesting "AND" logic gate approach (Figure 12). 60 In this probe (Si-NH 2 -Glu, Probe #18), glutamic acid was incorporated into the Si-NH 2 fluorophore through the typical γglutamyl linker. Interestingly, the NIR emission at 654 nm (λ ex = 515 nm) could be observed after GGT-catalyzed cleavage of γ-glutamyl linker at pH < 7.4.…”

Section: Near-infrared (Nir) Fluorescence Probe For Ggt Measurementmentioning

confidence: 99%

Advances and Perspectives of Responsive Probes for Measuring γ-Glutamyl Transpeptidase

Zhang,

et al. 2023

ACS Meas. Sci. Au

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Gamma-glutamyltransferase (GGT) is a plasma-membrane-bound enzyme that is involved in the γ-glutamyl cycle, like metabolism of glutathione (GSH). This enzyme plays an important role in protecting cells from oxidative stress, thus being tested as a key biomarker for several medical conditions, such as liver injury, carcinogenesis, and tumor progression. For measuring GGT activity, a number of bioanalytical methods have emerged, such as chromatography, colorimetric, electrochemical, and luminescence analyses. Among these approaches, probes that can specifically respond to GGT are contributing significantly to measuring its activity in vitro and in vivo. This review thus aims to highlight the recent advances in the development of responsive probes for GGT measurement and their practical applications. Responsive probes for fluorescence analysis, including "off−on", near-infrared (NIR), two-photon, and ratiometric fluorescence response probes, are initially summarized, followed by discussing the advances in the development of other probes, such as bioluminescence, chemiluminescence, photoacoustic, Raman, magnetic resonance imaging (MRI), and positron emission tomography (PET). The practical applications of the responsive probes in cancer diagnosis and treatment monitoring and GGT inhibitor screening are then highlighted. Based on this information, the advantages, challenges, and prospects of responsive probe technology for GGT measurement are analyzed.

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γ-Glutamyltranspeptidase and pH based “AND” logic gate fluorescent probe for orthotopic breast tumor imaging

Abstract: An “AND” logic gate-based NIR fluorescence probe Si-NH2-Glu was developed based on novel meso-amine Si-Rhodamine, which combined γ-glutamyl transpeptidase and pH dual-responsive sites. The feature of Si-NH2-Glu enable it to...

Cited by 11 publications

References 27 publications

Rational Design of Near-Infrared Fluorescent Probes for Accurately Tracking Lysosomal Viscosity with Allyl Snchoring Si-Rhodamine

Rational Design of Near-Infrared Fluorescent Probes for Accurately Tracking Lysosomal Viscosity with Allyl Snchoring Si-Rhodamine

Recent progress in the development of small-molecule double-locked logic gate fluorescence probes

Advances and Perspectives of Responsive Probes for Measuring γ-Glutamyl Transpeptidase

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