γ-Cyclodextrin-phenylacetic acid mesh as a drug trap

Transferrin-Conjugated pH-Responsive γ-Cyclodextrin Nanoparticles for Antitumoral Topotecan Delivery

“…Indeed, the TPT fluorescence intensities of γCDP-(DMA/PEG-Tf) NPs and γCDP-(PEG-Tf) NPs in CHO-K1 cells were noticeably shifted to low values (~67 and~78, respectively). These results indicate that the NPs with Tf ligands were efficiently endocytosed to MDA-MB-231 tumor cells [16,[32][33][34][35][36][37], which was also readily apparent in the confocal image results. respectively).…”

“…respectively). These results indicate that the NPs with Tf ligands were efficiently endocytosed to MDA-MB-231 tumor cells [16,[32][33][34][35][36][37], which was also readily apparent in the confocal image results. Figure 5b shows that the apoptotic cell population (Q2 and Q3) [16,30] of MDA-MB-231 cells treated with γCDP-(DMA/PEG-Tf) NPs was 55.7%, but those of MDA-MB-231 cells treated with γCDP-(DMA/PEG) NPs, γCDP-(PEG-Tf) NPs, and γCDP-(PEG) NPs were 27.8%, 41.6%, and 18.2%, respectively.…”

“…The particle size and zeta potential of each NP sample (0.1 mg/mL) was measured using a Zetasizer 3000 instrument (Malvern Instruments, Malvern, UK) after being stabilized in 150 mM PBS (pH 7.4, 6.5, or 6.0) at 25 • C for 4 h. The morphology of each NP sample was confirmed using a field-emission scanning electron microscopy (FE-SEM, Hitach S-400, Fukuoka, Japan) after being stabilized in 150 mM PBS (pH 7.4, 6.5, or 6.0) at 25 • C for 4 h [32][33][34][35][36][37].…”

Development of pH‐responsive cyclodextrin nanoparticles for tumor‐specific photodynamic therapy

“…1,5,[7][8][9] Among these polysaccharides, cyclodextrin (CD), a cyclic oligosaccharide, has excellent drug-inclusion/drug-affinity and magnificent biocompatibility, so it can be utilized in a variety of drug carriers through flexible host-guest interaction and its equilibrium process. [10][11][12][13][14] Furthermore, when combined with physicochemical properties that can elicit a tumor-specific response, CD-based carriers are expected to exhibit much more potent antitumor efficacy, promising site-specific drug release. [15][16][17] In particular, when introducing chemical functional groups capable of reacting to various stimuli (eg, weakly acidic pH in a tumor environment) in order to distinguish tumor cells, the likelihood of achieving this objective will be increased.…”

“…B, Synthesis scheme of γCD-(DEAP/PEG) [Colour figure can be viewed at wileyonlinelibrary.com] 2.2 | Synthesis of γCD conjugates γCD (1 g) was carboxylated with SA (1.75 g), DCC (6.3 g), and DMAP (1.9 g) in DMSO (70 mL) containing pyridine (1 mL) and TEA (1 mL) at 25 C for 4 days. 3,12,15 The resulting solution was dialyzed using preswollen dialysis membrane tubes (Spectra/Por MWCO 1 kDa) against fresh DMSO for 2 days and deionized water for 2 days to remove the non-reacted chemicals. 7,9,22 After lyophilizing, the carboxylated γCD (γCD-COOH, 0.5 g) was reacted with DEAP (0.6 g), DCC (1.8 g), and NHS (1 g) in DMSO (40 mL) containing pyridine (1 mL) and TEA (1 mL) at 25 C for 3 days.…”