β1 Integrin-mediated collagen gel contraction is stimulated by PDGF

Gullberg, Donald; Tingström, Anders; Thuresson, Ann‐Charlotte; Olsson, Lennart; Terracio, Louis; Borg, Thomas K.; Rubin, Kristofer

doi:10.1016/0014-4827(90)90305-t

Cited by 254 publications

(186 citation statements)

References 33 publications

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“…Preliminary isolation of the tumour-derived factor by means of gel infiltration has indicated that the same factor(s) is responsible for both matrix contraction and cell stretching and that its molecular weight is in the range of 45-65 kDa (Agrez, unpublished data). This is consistent with reports of several groups of investigators who have observed that the process of collagen lattice contraction appears linked to the process of fibroblast elongation (Buttle & Ehrlich, 1983;Guidry & Grinnell, 1985;Gullberg et al, 1990).…”

Section: Discussionsupporting

confidence: 93%

The role of colon fibroblasts in malignant large bowel obstruction – an experimental in vitro model

Agrez

Chua²

1990

Br J Cancer

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SummaryThe mechanism of bowel obstruction in colorectal cancer is likely to involve interactions between tumour cells, host fibroblasts and the extracellular matrix. The role of fibroblast-mediated matrix reorganisation in malignant structures of the large bowel was examined in an in vitro collagen matrix model in which tumour cells and fibroblasts were cultured under serum-free conditions. Colon cancer cells secreted a factor(s) which enhanced the ability of colon fibroblasts to contrast a collagen matrix without an associated mitogenic response by the fibroblasts. Within uncontracted collagen gels marked elongation of fibroblast cell processes was observed in the presence of the tumour-derived factor(s). We propose that matrix reorganisation by host fibroblasts in the wall of the human colon is responsible, at least in part, for malignant large bowel obstruction.Current views indicate that the presence of bowel obstruction adversely influences long-term survival from colorectal cancer and the detrimental effect on prognosis does not appear to be simply a function of more advanced tumour stage (Phillips et al., 1985;Chapuis et al., 1985;Fielding et al., 1986). One possibility is that malignant large bowel obstruction is related to tumour fibrosis since the latter has also been associated with a worse prognosis in rectal cancer (Jass et al., 1986). Although the mechanism of the fibrotic response observed in colorectal cancer remains unclear it is likely to involve not only deposition of new matrix, but also re-organisation of existing stroma.The extracellular matrix is composed largely of collagen, and cell-collagen binding is thought to be mediated by glycoprotein attachment molecules and proteoglycans (Yamada et al., 1985). Human colon carcinomas in organ culture have been found to synthesise proteoglycans and their production in the neoplastic colon appears to be localised to the stromal cell compartment rather than the epithelial compartment lozzo & Wight, 1982). This raises the possibility that colon cancer cells influence binding between host fibroblasts and the surrounding collagen matrix.A characteristic of fibroblasts is their ability to bind strongly to collagen and induce collapse of collagen matrices in vitro. This process is known as collagen lattice contraction, and has been considered analogous to wound contraction in vivo (Bell et al., 1979;Steinberg et al., 1980). Previous work undertaken in our laboratory has shown that human colon cancer cells from established cell lines do not cause significant collagen lattice contraction in vitro in contrast to normal human colon fibroblasts (Agrez, 1989a Fibroblast-mediated contraction of collagen discs Preparation of collagen gels Native type collagen was prepared by acetic acid extraction from rat tail tendons according to the method reported by van Bockxmeer and Martin (1982), and protein concentration estimated (Bio-RAD protein microassay, Bio-RAD Laboratories, CA, USA), according to a modification of the Lowry method (Lowry, et al., 1951). Collagen...

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Section: Discussionsupporting

confidence: 93%

The role of colon fibroblasts in malignant large bowel obstruction – an experimental in vitro model

Agrez

Chua²

1990

Br J Cancer

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“…To further localize the epitope of Br alloantigens, dissociation experiments with GPIa/IIa complex were performed. It has been demonstrated that treatment of the human fibroblast lysates with citrate buffer at pH 3.0 leads to dissociation of the #I integrin subunit from other a chains (23). Our preliminary experiments showed that under these conditions, platelet GPIa/ IIa chains did not completely dissociate from each other.…”

Section: Resultsmentioning

confidence: 60%

The human platelet alloantigens Br(a) and Brb are associated with a single amino acid polymorphism on glycoprotein Ia (integrin subunit alpha 2).

Santoso¹,

Kalb²,

Walka³

et al. 1993

J. Clin. Invest.

126

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The human GPIa/Ila complex, also known as integrin a2Bfl, serves as a major receptor for collagen in platelets and other cell types. In addition to its role in platelet adhesion to extracellular matrix, GPIa/IIa is also known to bear the clinically important Br' and Br' alloantigenic determinants, which can result in antibody-mediated platelet destruction. Immunochemical studies showed that the Br antigenic epitopes reside solely on the GP Ia subunit and do not depend on sialic acid residues. To define the polymorphism responsible for the Br alloantigen system platelet RNA PCR technique, was used to amplify GPIa mRNA transcripts. Nucleotide sequence analysis of the amplified platelet GPIa cDNA from Bra/a and Brb/b individuals revealed a single A * G polymorphism at base 1648. MnlI RFLP analysis of cDNA from serologically determined individuals confirmed that this polymorphism segregates with Br phenotype. This single base change results in a substitution of Lys (AAG) in Bra to Glu (GAG) in Brb at amino acid residue 505. In spite of the reversal in charge at this position, however, we found no difference in the ability of Bra and Br' homozygous platelets to adhere to collagens types I, III, or V, nor did antiBra or anti-Br' alloantibodies interfere with platelet adhesion to any of these fibrillar collagens. The identification of the nucleotide substitution that defines the Bra/Brb alloantigen system will now permit both pre-and postnatal diagnosis for Br phenotype. (J. Clin. Invest. 1993. 92:2427-2432

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“…Cultured human dermal fibroblasts will compact a gel to 10% of the initial gel area in 12-24 h. The process of collagen gel compaction depends on "1-integrins (Gullberg et al 1990) and on intercellular adhesions and Gap-junctions synchronizing the work (Ehrlich et al 2000). Several studies from our laboratory have shown that the characteristics of the collagen gel compaction mimic the cellular control of interstitial fluid pressure in vivo (Wiig et al 2003;Ahlen et al 1998;Berg et al 1998;Heuchel et al 1999).…”

Section: Discussionmentioning

confidence: 97%

“…The assay was performed essentially as described by Gullberg et al (1990). Explant ED/ES fibroblasts or Ag 1518-fibroblasts from confluent cultures were trypsinized, suspended in serum-free DMEM and diluted to 500,000 cells/ml.…”

Section: Collagen Gel Contraction Assaymentioning

confidence: 99%

“…wound healing (Heldin and Westermark 1999). PDGF-BB regulates interstitial fluid pressure in vivo (Rodt et al 1996) and promotes fibroblast adhesion to ECM in vitro in collagen gel contraction experiments (Clark et al 1989;Gullberg et al 1990). We have inferred that interstitial fluid pressure regulation is important in the inner ear and especially for endolymph resorption.…”

Section: Introductionmentioning

confidence: 99%

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An Interstitial Network of Podoplanin-Expressing Cells in the Human Endolymphatic Duct

Ekwall

Mayerl

Rubin

et al. 2006

JARO

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The human endolymphatic duct (ED) with encompassing interstitial connective tissue (CT) is believed to be important for endolymph resorption and fluid pressure regulation of the inner ear. The periductal CT cells are interconnected via numerous cellular extensions, but do not form vessel structures. Here we report that the periductal CT is populated by two distinct cell phenotypes; one expressing podoplanin, a protein otherwise found on lymph endothelia and on epithelia involved in fluid fluxes, and a second expressing a fibroblast marker. A majority of the interstitial cells expressed podoplanin but not the lymphatic endothelial cell markers hyaluronan receptor (LYVE-1) or vascular endothelial growth factor receptor-3 (VEGFR-3). The fibroblast marker positive cells were found close to the ED epithelium. In the mid-and distal parts of the ED, these cells were enriched under folded epithelia. Furthermore, subepithelial CT cells were found to express activated platelet derived growth factor (PDGF)-" receptors. Cultured CT cells from human inner ear periductal and perisaccular explant tissues were identified as fibroblasts. These cells compacted a three-dimensional collagen lattice by a process that could be promoted by PDGF-BB, a factor involved in interstitial fluid pressure regulation. Our results are compatible with the notion that the periductal CT cells are involved in the regulation of inner ear fluid pressure. By active compaction of the periductal CT and by the formation of villous structures, the CT cells could modulate fluid fluxes over the ED epithelium as well as the longitudinal flow of endolymph in the ED.

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β1 Integrin-mediated collagen gel contraction is stimulated by PDGF

Cited by 254 publications

References 33 publications

The role of colon fibroblasts in malignant large bowel obstruction – an experimental in vitro model

The role of colon fibroblasts in malignant large bowel obstruction – an experimental in vitro model

The human platelet alloantigens Br(a) and Brb are associated with a single amino acid polymorphism on glycoprotein Ia (integrin subunit alpha 2).

An Interstitial Network of Podoplanin-Expressing Cells in the Human Endolymphatic Duct

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