β-Lapachone activity in synergy with conventional antimicrobials against methicillin resistant Staphylococcus aureus strains

Abstract

The aim of this study was to evaluate the antimicrobial activity of lapachol, α-lapachone, β-lapachone and six antimicrobials (ampicillin, amoxicillin/clavulanic acid, cefoxitin, gentamicin, ciprofloxacin and meropenem) against twelve strains of Staphylococcus aureus from which resistance phenotypes were previously determined by the disk diffusion method. Five S. aureus strains (LFBM 01, LFBM 26, LFBM 28, LFBM 31 and LFBM 33) showed resistance to all antimicrobial agents tested and were selected for the study of the interaction between β-lapachone and antimicrobial agents, busing checkerboard method. The criteria used to evaluate the synergistic activity were defined by the Fractional Inhibitory Concentration Index (FICI). Among the naphthoquinones, β-lapachone was the most effective against S. aureus strains. FICI values ranged from 0.07 to 0.5, suggesting a synergistic interaction against multidrug resistant S. aureus (MRSA) strains. An additive effect was observed with the combination β-lapachone/ciprofloxacin against the LFBM 33 strain. The combination of β-lapachone with cefoxitin showed no added benefit against LFBM 31 and LFBM 33 strains. This study demonstrated that, in general, β-lapachone combined with beta lactams antimicrobials, fluoroquinolones and carbapenems acts synergistically inhibiting MRSA strains.

Antimicrobial activity and FTIR characterization of lapachol derivatives against Staphylococcus aureus

“…This shows the clinical importance of this study which allows a focused therapeutic treatment in hospital infections cases (Colli et al, 2009). This studies also corroborate with Macedo et al (2013). These evaluated the antimicrobial activity of lapachol, α-lapachone, β-lapachone six antibiotics (Ampicillin, Amoxicillin/Clavulanic Acid, Cefoxitin, Gentamicin, Ciprofloxacin and Meropenen) against 20 strains of S. aureus that presented phenotypic resistance when subjected to the disk diffusion method.…”

“…These evaluated the antimicrobial activity of lapachol, α-lapachone, β-lapachone six antibiotics (Ampicillin, Amoxicillin/Clavulanic Acid, Cefoxitin, Gentamicin, Ciprofloxacin and Meropenen) against 20 strains of S. aureus that presented phenotypic resistance when subjected to the disk diffusion method. In this work, the β-lapachone as compared to other substances (lapachol and α-lapachone) showed a strong antibacterial effect against MRSA strains (methicillin-resistant S. aureus) with values of 8-32 μg/mL in the Minimum Inhibitory Concentration (MIC) (Macedo et al, 2013).…”

“…These numerous activities are related to chemical and structural details of naphthoquinones. Thus, some researchers are developing structural changes in the lapachol molecule to improve its biological activity (Araújo, 2002;Macedo et al, 2013).…”

Produtos da Agrobiodiversidade: uma análise da qualidade dos sabonetes líquidos de plantas medicinais produzidos por agricultores familiares da Zona Oeste do Rio de Janeiro

“…Desencadeiam infecções simples como as espinhas, furúnculos e celulites como até mesmo as infecções mais graves como pneumonia, meningite, endocardite, síndrome do choque tóxico e septicemia (41) .…”

β-Lapachone suppresses the lung metastasis of melanoma via the MAPK signaling pathway

“…It is used as herbal medicine by indigenous people in South and Central America. This compound has various pharmacological effects including anti-bacterial, anti-viral, and anti-inflammatory activities [13–15]. In addition, β-lapachone exhibited an anti-cancer effect in various types of cancer, including liver, breast, prostate, and colon cancer [16–19].…”