β-Lactamases of
            <i>Kluyvera ascorbata</i>
            , Probable Progenitors of Some Plasmid-Encoded CTX-M Types

Humeniuk, Christel; Arlet, Guillaume; Gautier, Valérie; Grimont, Patrick A. D.; Labia, Roger; Philippon, A.

doi:10.1128/aac.46.9.3045-3049.2002

Cited by 289 publications

(185 citation statements)

References 24 publications

(26 reference statements)

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“…These enzymes are not closely related to others families of ESBLs (TEM and SHV), but share a high level of identity with chromosomal β-lactamases derived from Kluyvera spp. [17]. CTX-M-type ESBLs confer higher levels of resistance to cefotaxime than to ceftazidime.…”

Section: Pcr-based Amplification Of the Bla Ctx-mmentioning

confidence: 99%

ESBL-Producing Escherichia coli Isolated from Bloodstream Infections – Antimicrobial Susceptibility, Conjugative Transfer of Resistance Genes and Phylogenetic Origin

Franiczek¹,

Krzyżanowska²

2014

Adv Clin Exp Med

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Background. The prevalence of bloodstream infections (BSIs) due to ESBL-producing Escherichia coli (ESBL-EC) strains has increased dramatically over the past years. Objectives. Characterization of ESBL-EC isolates collected from BSIs with regard to their antimicrobial susceptibility and phylogenetic background. The conjugative transfer of resistance determinants to the E. coli reference strain K12 C600 was also investigated. Material and Methods. A collection of forty-eight ESBL-EC strains recovered from BSIs was subjected to the study. These strains were obtained from the ICU (intensive care unit) of the Medical University Hospital, Wrocław, Poland, during a four-year period (2009)(2010)(2011)(2012). All the isolates were screened for ESBL production by the double disk synergy test (DDST). Transferability of plasmid-mediated resistance genes was performed by the conjugational broth method. Susceptibility to antibiotics and chemotherapeutics of clinical isolates and transconjugants was determined by the agar dilution method. PCR assay was used to detect the bla CTX-M gene in ESBL-EC tested and transconjugants. Affiliation to phylogenetic groups was done by the triplex PCR method. Results. Conjugational transfer of plasmids responsible for ESBL to a recipient strain was successful for all the ESBL-EC analyzed (donors). The conjugation frequencies ranging from 2.3 × 10 -7 to 5.2 × 10 -1 per donor. In vitro susceptibility testing revealed that all the ESBL-EC isolates and their transconjugants were resistant to most of the antimicrobial agents tested with the exception of carbapenems, tigecycline, and β-lactam-clavulanate combinations. Moreover, all the donor strains and their transconjugants were found to contain the bla CTX-M gene. The majority of the isolates analyzed belonged to phylogroups B2 (62.5%) and D (25%), whereas groups B1 and A were less frequently represented (8.3% and 4.2%, respectively). Conclusions. The results of the study confirm the need of antibiotic policies and effective infection control measures in hospital settings to minimize BSIs caused by multi-resistant ESBL-producing pathogens (Adv Clin Exp Med 2014, 23, 6, 865-870).

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Section: Pcr-based Amplification Of the Bla Ctx-mmentioning

confidence: 99%

ESBL-Producing Escherichia coli Isolated from Bloodstream Infections – Antimicrobial Susceptibility, Conjugative Transfer of Resistance Genes and Phylogenetic Origin

Franiczek¹,

Krzyżanowska²

2014

Adv Clin Exp Med

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“…The CTX-M group consist of five groups, each group contains different types. Beta-Lactamases of the CTX-M group II are structurally related to the naturally produced beta-lactamase of Kluyvera ascorbata (6), and in CTX-M group III, is related to beta-lactamase of K. georgiana (7), and CTX-M group I enzymes are related to the beta-lactamases of K. cryocrescens (8). Although an identical enzyme to CTX-M-3 was isolated from K.ascorbata (9).…”

Section: Introductionmentioning

confidence: 99%

Dissemination of CTX-M-Type Beta-lactamase Among Clinical Isolates of Enterobacteriaceae in Markazi Province, Iran

Safari

Shojapour

Akbari

et al. 2013

Jundishapur J Microbiol

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Background: Organisms producing CTX-M-lactamases are known as the source of resistance to Oxyiminocephalosporins such as Eeftriaxone and Ceftazidime. However, the laboratory detection of these strains is not well defined. Objectives: The aim of this study was to determine the presence and prevalence of known CTX-M-beta-beta-lactamase genes in clinical isolates of Enterobacteriaceae from Arak educational hospitals, Iran. Materials and Methods: During a 10-month period (May to February 2010), 350 randomly Enterobacteriaceae isolates were obtained from the clinical laboratories of different hospitals of Arak University of Medical Sciences, Iran. Antibiotic susceptibility was tested by CLSI disk diffusion and extended spectrum beta-lactamase (ESBL) confirmatory tests. Minimum Inhibitory Concentration (MICs) was determined by broth micro dilution. All of the ESBL-producing isolates were examined by PCR to detect the presence of bla CTX-M genes. Results: In phenotypic confirmatory test, 154 (44%) out of 350 clinical isolates were ESBL positive. Using molecular assay, 154 strains potentially producing extended-spectrum-beta -lactamases were examined for the presence of CTX-M enzymes. 92.2% isolates CTX-M -1, 28.5% isolates CTX-M-2, 17.5% isolates CTX-M-8, and 38.3% isolates CTX-M-9 genes detected by PCR. Conclusions:The levels of resistance to Ceftazidime were remarkably variable among CTX-Mproducers. This study provides further evidences of the global dissemination of CTX-M type ESBLs and emphasized on the need for their epidemiological monitoring.

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“…Within each cluster there is a high degree of bla CTX-M gene sequence identity (Ͼ95%), although members of different subtypes share only 70 to 77% similarity at the nucleotide level. The recent finding of Kluyvera ascorbata species-specific ␤-lactamases (KluA) which share 98.6 to 100% identity with CTX-M-2 and CTX-M-5 and analysis of DNA sequences adjacent to the KluA-and CTX-Mcoding genes provide strong evidence of the direct evolution of the CTX-M-2 cluster from the chromosomal enzyme of K. ascorbata (20,36). Likewise, the chromosomal KLUG-1 enzyme of Kluyvera georgiana was found to be the most-probable progenitor of the CTX-M-8 that shares 99% identity with KLUG-1 (32).…”

mentioning

confidence: 99%

Prevalence and Molecular Epidemiology of CTX-MExtended-Spectrum β-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae in RussianHospitals

Edelstein

Pimkin

Palagin

et al. 2003

Antimicrob Agents Chemother

320

180

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A total of 904 consecutive nosocomial isolates of Escherichia coli and Klebsiella pneumoniae collected from 28 Russian hospitals were screened for production of extended-spectrum ␤-lactamases (ESBLs). The ESBL phenotype was detected in 78 (15.8%) E. coli and 248 (60.8%) K. pneumoniae isolates. One hundred fifteen isolates carried the genes for CTX-M-type ␤-lactamases, which, as shown by PCR-restriction fragment length polymorphism analysis, were distributed into the two genetic groups of CTX-M-1 (93%)-and CTX-M-2 (7%)-related enzymes. Isolates producing the enzymes of the first group were found in 20 hospitals from geographically distant regions of the country and were characterized by considerable diversity of genetic types, as was demonstrated by enterobacterial repetitive consensus PCR typing. Within this group the CTX-M-3 and the CTX-M-15 ␤-lactamases were identified. In contrast, the enzymes of the CTX-M-2 group (namely, CTX-M-5) were detected only in eight clonally related E. coli isolates from a single hospital. Notably, the levels of resistance to ceftazidime were remarkably variable among the CTX-M producers. This study provides further evidence of the global dissemination of CTX-M type ESBLs and emphasizes the need for their epidemiological monitoring.During the past decade extended-spectrum ␤-lactamases (ESBLs) of the CTX-M type emerged in many countries of the world. The first organisms producing ␤-lactamases of this type were identified both as single and epidemic clinical isolates in very distant geographic regions (Germany and France and Argentina) in the early 1990s (3, 5, 7). More recently, a rapid increase in the proportion of multiple CTX-M variants to the TEM-and SHV-derived ESBLs has been reported in many hospitals in Spain (9,12,15,35) (14), and Korea (28). Furthermore, CTX-M ␤-lactamases, mainly types CTX-M-2 and CTX-M-3, were found to be widespread or even predominant ESBL types in several countries, including Argentina (31, 34; M. Galas, F. Pasteran, R. Melano, A. Petroni, G. Lopez, A. Corso, A. Rossi, et al., Abstr. 38th Intersci. Conf. Antimicrob. Agents Chemother., abstr. E-109, 1998), Japan (41), and Poland (1).Currently the CTX-M family includes more than 20 ␤-lactamases, which may be grouped on the basis of sequence similarity into four distinct clusters (subtypes) epitomized by CTX-M-1, CTX-M-2, CTX-M-8, and CTX-M-9 (26, 37; http: //www.lahey.org/studies/webt.htm). Within each cluster there is a high degree of bla CTX-M gene sequence identity (Ͼ95%), although members of different subtypes share only 70 to 77% similarity at the nucleotide level. The recent finding of Kluyvera ascorbata species-specific ␤-lactamases (KluA) which share 98.6 to 100% identity with CTX-M-2 and CTX-M-5 and analysis of DNA sequences adjacent to the KluA-and CTX-Mcoding genes provide strong evidence of the direct evolution of the CTX-M-2 cluster from the chromosomal enzyme of K. ascorbata (20,36). Likewise, the chromosomal KLUG-1 enzyme of Kluyvera georgiana was found to be the most-probable progenitor of ...

show abstract

β-Lactamases of Kluyvera ascorbata , Probable Progenitors of Some Plasmid-Encoded CTX-M Types

Cited by 289 publications

References 24 publications

ESBL-Producing Escherichia coli Isolated from Bloodstream Infections – Antimicrobial Susceptibility, Conjugative Transfer of Resistance Genes and Phylogenetic Origin

ESBL-Producing Escherichia coli Isolated from Bloodstream Infections – Antimicrobial Susceptibility, Conjugative Transfer of Resistance Genes and Phylogenetic Origin

Dissemination of CTX-M-Type Beta-lactamase Among Clinical Isolates of Enterobacteriaceae in Markazi Province, Iran

Prevalence and Molecular Epidemiology of CTX-MExtended-Spectrum β-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae in RussianHospitals

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