β-Lactamase Genes without Limits

Naas, Thierry; Dabos, Laura; Bonnin, Rémy A.

doi:10.3390/microorganisms11051200

Cited by 1 publication

(1 citation statement)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The presence of OXA-48-carbapenemase producers is very worrying, as carbapenems remain the last-resort therapy for treating human infections caused by MDR Gram-negative and Gram-positive bacteria [ 31 ]. However, the clinical use of these antibiotics is presently at risk due to the global proliferation of β-lactamases (BLs) with the ability to degrade them, and the increase in the worldwide emergence of carbapenem-resistant organisms (CROs), which constitute a critical growing public health threat [ 34 ]. In livestock or veterinary fields, carbapenems are not licensed and have no legal indication, so their use is prohibited [ 32 , 35 , 36 ].…”

Section: Discussionmentioning

confidence: 99%

Antibiotic Susceptibility Profiles of Bacterial Isolates Recovered from Abscesses in Cattle and Sheep at a Slaughterhouse in Algeria

Yousfi,

Oueslati,

Daaboul

et al. 2024

Microorganisms

Self Cite

View full text Add to dashboard Cite

Abscesses represent the most prominent emerging problem in the red meat industry, leading to great economic constraints and public health hazards. Data on etiological agents present in these purulent lesions in Algeria are very scarce. The aim of this study was to identify the bacteria responsible for these abscesses and to determine their antibiotic susceptibility profiles. A total of 123 samples of abscesses from 100 slaughtered sheep and 23 slaughtered cattle were cultured in several media. A total of 114 bacterial isolates were cultured from 103 abscesses. Bacteria were identified using MALDI–TOF, and antibiotic susceptibility was determined by the disk diffusion method on Mueller–Hinton agar. A total of 73.6% (n = 84) corresponded to Enterobacterales, of which four were multidrug-resistant (MDR). These isolates, together with Staphylococcus aureus, coagulase negative Staphylococci, and seven randomly chosen susceptible Escherichia coli isolates, were further characterized using WGS. Resistome analysis of the four MDR Enterobacterales isolates revealed the presence of OXA-48 carbapenemase in two Klebsiella pneumoniae ST985 and one E. coli ST10 isolates and a CTX-M-15 ESBL in one E. coli isolate ST1706. Two coagulase-negative Staphylococci isolates were found to carry the mecA gene. WGS showed the presence of different resistance genes and virulence genes. Our study revealed 5% of MDR Enterobacterales (including ESBLs and carbapenemases) identified from abscesses, thus urging the need for abscess monitoring in slaughterhouses.

show abstract