β-lactam resistance genes in bacteriophage and bacterial DNA from wastewater, river water, and irrigation water in Washington State

Zhang, Anyun; Call, Douglas R.; Besser, Thomas E.; Liu, Jinxin; Jones, Lisa P.; Wang, Hongning; Davis, Margaret A.

doi:10.1016/j.watres.2019.06.026

Cited by 33 publications

(20 citation statements)

References 37 publications

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“…They found that the abundance of ARGs carried by phages is higher in samples collected from hospital discharge points and wastewater treatment plants (WWTPs), indicating that sewage may be an important source of ARGs (Yang et al, 2018). Another study also found that the abundance of βlactamase genes in phages is higher in sewage samples (Zhang et al, 2019). This makes it necessary to consider the role of sewage in promoting transduction by phages.…”

Section: Phage-mediated Horizontal Gene Transfermentioning

confidence: 99%

“…Crossassembly phage and pepper mild mottle virus as viral water quality monitoring tools-potential, research gaps, and way forward. Current Opinion in Environmental Science & Health, 16: 54-61 Brown-Jaque M, Calero-Cáceres W, Espinal P, Rodríguez-Navarro J, Miró E, González-López J J, Cornejo T, Hurtado J C, Navarro F, Larrañaga et al, 2018 The Funan River aac(6′)-lb-cr, aph(3′)-IIIa, bla CTX-M , ermF, sul1, sul2 Yang et al, 2018 Sewage from WWTP bla TEM , bla OXA-48 , bla PSE , bla CTX-M , bla KPC , bla CMY-2 , bla NDM-1 Zhang et al, 2019 Chicken feces aac(6′)-lb-cr, aph(3′)-IIIa, bla CTX-M , ermB, ermF, floR, mcr-1, qnrS, sul1, sul2, tetM, vanA Yang et al, 2020 Human fecal samples bla TEM , bla CTX-M-1 , bla CTX-M-9 , armA, qnrA, qnrS, sul1, bla OXA-48 , mecA Brown-Jaque et al, 2018…”

Section: Referencesmentioning

confidence: 99%

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Bacteriophages in water pollution control: Advantages and limitations

Liu

Sun

et al. 2020

Front. Environ. Sci. Eng.

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Section: Phage-mediated Horizontal Gene Transfermentioning

confidence: 99%

Section: Referencesmentioning

confidence: 99%

Bacteriophages in water pollution control: Advantages and limitations

Liu

Sun

et al. 2020

Front. Environ. Sci. Eng.

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“…The bla TEM gene was the most prominent ARG in the bacteriophage fraction, followed by sul1, bla SHV , and qnrS. Resistance to β-lactam antimicrobial agents by bla TEM , bla SHV are widely distributed in aquatic ecosystems (Colomer-Lluch et al, 2011;Anand et al, 2016;Calero-Cáceres et al, 2017;Zhang et al, 2019), which might indicate that they are particularly amenable to propagation through transduction (Wang et al, 2018a). Sulfonamides are one of the oldest antimicrobial synthetic or semi-synthetic drug classes that have been also used for the treatment of animals (Dasenaki and Thomaidis, 2017) and is an authorized antibiotic for use in aquaculture in Europe (Santos and Ramos, 2018).…”

Section: Discussionmentioning

confidence: 99%

Bacterial and Bacteriophage Antibiotic Resistance in Marine Bathing Waters in Relation to Rivers and Urban Streams

et al. 2021

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Fecal pollution of surface water may introduce bacteria and bacteriophages harboring antibiotic resistance genes (ARGs) into the aquatic environment. Watercourses discharging into the marine environment, especially close to designated bathing waters, may expose recreational users to fecal pollution and therefore may increase the likelihood that they will be exposed to ARGs. This study compares the bacterial and bacteriophage ARG profiles of two rivers (River Tolka and Liffey) and two small urban streams (Elm Park and Trimleston Streams) that discharge close to two marine bathing waters in Dublin Bay. Despite the potential differences in pollution pressures experienced by these waterways, microbial source tracking analysis showed that the main source of pollution in both rivers and streams in the urban environment is human contamination. All ARGs included in this study, blaTEM, blaSHV, qnrS, and sul1, were present in all four waterways in both the bacterial and bacteriophage fractions, displaying a similar ARG profile. We show that nearshore marine bathing waters are strongly influenced by urban rivers and streams discharging into these, since they shared a similar ARG profile. In comparison to rivers and streams, the levels of bacterial ARGs were significantly reduced in the marine environment. In contrast, the bacteriophage ARG levels in freshwater and the marine were not significantly different. Nearshore marine bathing waters could therefore be a potential reservoir of bacteriophages carrying ARGs. In addition to being considered potential additional fecal indicators organism, bacteriophages may also be viewed as indicators of the spread of antimicrobial resistance.

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“…Initially, their absence was noted on the basis of single phage biology methods, such as phage culturing and sequencing, and therefore the investigations lacked breadth. On the other hand, qPCR studies have reported the presence of ARGs in virus-enriched environmental samples from natural waters 6 , wastewater plants 7 , or human/animal fecal samples 8,9 . However, virome preparations are often highly contaminated by bacterial DNA 10,11 , making it difficult to discern whether the ARG originates from viral or bacterial DNA using qPCR alone.…”

Section: Introductionmentioning

confidence: 99%

Analysis of viromes and microbiomes from pig fecal samples reveals that phages and prophages are not vectors of antibiotic resistance genes

Billaud

Lamy-Besnier

Lossouarn

et al. 2021

Preprint

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Understanding the transmission of antibiotic resistance genes (ARGs) is critical for human health. For this, it is necessary to identify which type of mobile genetic elements is able to spread them from animal reservoirs into human pathogens. Previous research suggests that in pig feces, ARGs may be encoded by bacteriophages. However, convincing proof for phage-encoded ARGs in pig viromes is still lacking, because of bacterial DNA contaminating issues. We collected 14 pig fecal samples and performed deep sequencing on both highly purified viral fractions and total microbiota, in order to investigate phage and prophage-encoded ARGs. We show that ARGs are absent from the genomes of active, virion-forming phages (below 0.02% of viral contigs from viromes), but present in three prophages, representing 0.02% of the viral contigs identified in the microbial dataset. However, the corresponding phages were not detected in the viromes, and their genetic maps suggest they might be defective. Furthermore, our dataset allows for the first time a comprehensive view of the interplay between prophages and viral particles.

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β-lactam resistance genes in bacteriophage and bacterial DNA from wastewater, river water, and irrigation water in Washington State

Cited by 33 publications

References 37 publications

Bacteriophages in water pollution control: Advantages and limitations

Bacteriophages in water pollution control: Advantages and limitations

Bacterial and Bacteriophage Antibiotic Resistance in Marine Bathing Waters in Relation to Rivers and Urban Streams

Analysis of viromes and microbiomes from pig fecal samples reveals that phages and prophages are not vectors of antibiotic resistance genes

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