β-Lactam Antibiotics Production with Penicillium chrysogenum and Acremonium chrysogenum

Bellgardt, K.‐H.

doi:10.1007/978-3-642-59735-0_14

Cited by 1 publication

(1 citation statement)

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“…The other morphological parameters will be useful to characterize the morphological differentiation steps. For instance, it is known that cultures exhibiting a high rate of differentiation from swollen hyphae to arthrospores tend to produce more antibiotics than fermentations adopting the filamentous shape (30).…”

Section: Discussionmentioning

confidence: 99%

Morphological Characterization and Viability Assessment of Trichoderma reesei by Image Analysis

Lecault

Patel

Thibault

2008

Biotechnol Progress

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The production of cellulase from the filamentous fungus Trichoderma reesei is a critical step in the industrial process leading to cellulose ethanol. As a result of the lack of quantitative analysis tools, the intimate relationship that exists between the morphological and physiological states of the microorganism, the shear field in the bioreactor, and the process performance is not yet fully understood. A semiautomatic image analysis protocol was developed to characterize the mycelium morphology and to estimate its percentage viability during the fermentation process based on four morphological types (unbranched, branched, entangled, and clumped microorganisms). Pictures taken under bright field microscopy combined with images of fluorescein diacetate stained fungi were used to assess the morphological parameters and the percentage viability of microorganisms simultaneously. The method was tested during the course of fed-batch fermentation in a reciprocating plate bioreactor. The use of the image analysis protocol was found to be successful in quantifying the variations in the morphology and the viability of T. reesei throughout the fermentation.

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Section: Discussionmentioning

confidence: 99%