β-Ionone attenuates LPS-induced pro-inflammatory mediators such as NO, PGE2 and TNF-α in BV2 microglial cells via suppression of the NF-κB and MAPK pathway

Kang, Chang‐Hee; Jayasooriya, Rajapaksha Gendara Prasad Tharanga; Choi, Yung Hyun; Moon, Sung‐Kwon; Kim, Wun-Jae; Kim, Gi‐Young

doi:10.1016/j.tiv.2012.12.012

Cited by 42 publications

(21 citation statements)

References 35 publications

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“…U0126, a specific inhibitor for MEK1/2, the kinases leading to phosphorylation of ERK1/2, readily abrogated IFNγ induced NO in these cells. Besides induction of ROS and NO, microglia activation is also accompanied by secretion of pro-inflammatory cytokines and chemokines, including TNFα, IL-1β and IL-6 (Kang et al, 2013; Lim, et al, 2012). Many botanical compounds offer neuroprotective effects by suppressing microglial activation.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of microglial activation by elderberry extracts and its phenolic components

et al. 2015

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Aims Elderberry (Sambucus spp.) is one of the oldest medicinal plants noted for its cardiovascular, anti-inflammatory, and immune-stimulatory properties. In this study, we investigated the anti-inflammatory and anti-oxidant effects of the American elderberry (Sambucus nigra subsp. canadensis) pomace as well as some of the anthocyanins (cyanidin chloride and cyanidin 3-O-glucoside) and flavonols (quercetin and rutin) in bv-2 mouse microglial cells. Main methods The bv-2 cells were pretreated with elderberry pomace (extracted with ethanol or ethyl acetate) or its anthocyanins and flavonols and stimulated by either lipopolysaccharide (LPS) or interferon-γ (IFNγ). Reactive oxygen species (ROS) and nitric oxide (NO) production (indicating oxidative stress and inflammatory response) were measured using the ROS detection reagent DCF-DA and the Griess reaction, respectively. Key findings Analysis of total monomeric anthocyanin (as cyanidin 3-O-glucoside equivalents) indicated five-fold higher amount in the freeze-dried ethanol extract as compared to that of the oven-dried extract; anthocyanin was not detected in the ethyl acetate extracts. Elderberry ethanol extracts (freeze-dried or oven-dried) showed higher anti-oxidant activities and better ability to inhibit LPS or IFNγ-induced NO production as compared with the ethyl acetate extracts. The phenolic compounds strongly inhibited LPS or IFNγ-induced ROS production, but except for quercetin, they were relatively poor in inhibiting NO production. Significance These results demonstrated difference in anti-oxidative and anti-inflammatory effects of elderberry extracts depending on solvents used. Results further identified quercetin as the most active component in suppressing oxidative stress and inflammatory responses on microglial cells.

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Section: Discussionmentioning

confidence: 99%

Inhibition of microglial activation by elderberry extracts and its phenolic components

et al. 2015

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“…2 Many studies have demonstrated that activated macrophages, key inammatory cells, play a pivotal role in diseases via the secretion of various inammatory mediators such as nitric oxide (NO) and prostaglandin (PG) E2, as well as pro-inammatory cytokines, including tumor necrosis factor-a (TNF-a), interleukin-1beta (IL-1b) and interleukin-6 (IL-6). 3 Studies in animal models and patients suggest that pro-inammatory factors, including NO, TNF-a, PGE2 and IL-6 may be produced by macrophages or by the surrounding tissues, and also induce the expression of cyclooxygenase-2 (COX-2) and inducible NO synthase (iNOS), which leads to the up-regulation of NO and PGE2. 4 The activated forms of COX, namely COX-1 and COX-2, catalyze the production of leukotrienes, which are arachidonic acid metabolites.…”

Section: Introductionmentioning

confidence: 99%

Anti-inflammatory potency of Locusta migratoria manilensis cyclopeptides in mast cells and macrophages

Liu

Wang

et al. 2019

RSC Adv.

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“…MAPKs and NF‐κB pathways are known to play a critical role in the LPS‐induced inflammatory response in microglia . We found that Z‐590 inhibited the LPS‐stimulated MAPKs phosphorylation, including p‐ERK, p‐p38 and p‐JNK, but didn't alter the IκBα level.…”

Section: Discussionmentioning

confidence: 77%

“…Interestingly, LPS was found to alter MIF receptors expression especially CD74 and CXCR2 in BV-2 microglia (Figure 1), suggesting that MIF may also be involved MAPKs and NF-κB pathways are known to play a critical role in the LPS-induced inflammatory response in microglia. 33,34 We found that Z-590 inhibited the LPS-stimulated MAPKs phosphorylation, including p-ERK, p-p38 and p-JNK, but didn't alter the IκBα level. This was further supported by the data from nuclear expressions of p65 and p-c-Jun in BV-2 microglia cells ( Figure 4).…”

Section: Discussionmentioning

confidence: 79%

Inhibition of macrophage migration inhibitory factor (MIF) tautomerase activity suppresses microglia‐mediated inflammatory responses

Zhang

Jia

et al. 2016

Clin Exp Pharma Physio

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Macrophage migration inhibitory factor (MIF), a pleiotropic pro-inflammatory cytokine, is a key regulator in both innate and acquired immunity systems. MIF has become a promising drug target for inflammatory diseases. Apart from its cytokine activities, MIF is known to act as a d-dopachrome tautomerase. Our previous work has identified that 3-[(biphenyl-4-ylcarbonyl)carbamothioyl]amino benzoic acid (Z-590) exhibited a potent inhibitory activity against MIF. In this study, we investigate the effect of Z-590 on lipopolysaccharide (LPS)-activated microglial cell activation. Our results demonstrate that Z-590 significantly decreases the production of nitric oxide (NO), tumour necrosis factor-alpha (TNF-α), interleukin (IL)-6, IL-1β, cyclooxygenase (COX-2), inducible nitric oxide synthase (iNOS) as well as reactive oxygen species (ROS) involved in inhibiting MAKPs signalling pathway in LPS-stimulated microglia cells. Furthermore, Z-590 reduced cytotoxicity of activated microglia toward HT-22 hippocampal cells in a microglia-conditioned medium system. Taken together, these results indicate that MIF inhibitor Z-590 elicits a potent inhibitor for microglia-mediated neuroinflammation.

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β-Ionone attenuates LPS-induced pro-inflammatory mediators such as NO, PGE2 and TNF-α in BV2 microglial cells via suppression of the NF-κB and MAPK pathway

Cited by 42 publications

References 35 publications

Inhibition of microglial activation by elderberry extracts and its phenolic components

Inhibition of microglial activation by elderberry extracts and its phenolic components

Anti-inflammatory potency of Locusta migratoria manilensis cyclopeptides in mast cells and macrophages

Inhibition of macrophage migration inhibitory factor (MIF) tautomerase activity suppresses microglia‐mediated inflammatory responses

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