β-Hydroxybutyrate Alleviates Low Glucose–Induced Apoptosis via Modulation of ROS-Mediated p38 MAPK Signaling

Li, Cixia; Chai, Xuejun; Pan, Jiarong; Huang, Jian; Wu, Yanjun; Xue, Yuhuan; Zhou, Wentai; Yang, Jiping; Zhu, Xiaoyan; Zhao, Shanting

doi:10.1007/s12031-022-01974-3

Cited by 2 publications

(2 citation statements)

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“…It has been reported that glucose deprivation leads to ATP depletion and apoptotic pathway activation. 52 This prompted us to analyze the effect of NO signaling on apoptosis in HT22 cells. Cultured in 1 mM glucose for 6 h, HT22 cells exhibited a significant increase in apoptotic cells, as revealed by the TUNEL assay, 53 indicating a close association between NO signaling and hypoglycemia-induced apoptosis ( Fig.…”

Section: Resultsmentioning

confidence: 99%

“…[49][50][51] To explore the potential modulation of neurological damage under hypoglycemic conditions by NO signaling, HT22 cells were cultured in a 1 mM glucose medium for 6 h to mimic hypoglycemia. 52 Cells were then stained either with NOP-1 or the commercial NO probe DAF-FM DA. It was observed that either probe consistently exhibited weak uorescence in high-glucose-treated cells but strong uorescence in low-glucose-treated cells (Fig.…”

Section: Nop-1 Imaging Revealed the Implication Of No Signaling In Hy...mentioning

confidence: 99%

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Deconvoluting nitric oxide–protein interactions with spatially resolved multiplex imaging

Li,

Pan,

Gao

et al. 2024

Chem. Sci.

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Section: Resultsmentioning

confidence: 99%

Section: Nop-1 Imaging Revealed the Implication Of No Signaling In Hy...mentioning

confidence: 99%

Deconvoluting nitric oxide–protein interactions with spatially resolved multiplex imaging

Li,

Pan,

Gao

et al. 2024

Chem. Sci.

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Low Glucose plus β-Hydroxybutyrate Induces an Enhanced Inflammatory Response in Yak Alveolar Macrophages via Activating the GPR109A/NF-κB Signaling Pathway

Yang

Xia

et al. 2023

IJMS

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Yaks are often subject to long-term starvation and a high prevalence of respiratory diseases and mortality in the withered season, yet the mechanisms that cause this remain unclear. Research has demonstrated that β-hydroxybutyrate (BHB) plays a significant role in regulating the immune system. Hence, we hypothesize that the low glucose and high BHB condition induced by severe starvation might have an effect on the pro-inflammatory response of the alveolar macrophages (AMs) in yaks. To validate our hypothesis, we isolated and identified primary AMs from freshly slaughtered yaks and cultured them in a medium with 5.5 mM of glucose or 2.8 mM of glucose plus 1–4 mM of BHB. Utilizing a real-time quantitative polymerase chain reaction (RT-qPCR), immunoblot assay, and enzyme-linked immunosorbent assay (ELISA), we evaluated the gene and protein expression levels of GPR109A (G-protein-coupled receptor 109A), NF-κB p65, p38, and PPARγ and the concentrations of pro-inflammatory cytokines interleukin (IL)-1β and IL-6 and tumor necrosis factor (TNF)-α in the supernatant. The results demonstrated that AMs exposed to low glucose plus BHB had significantly higher levels of IL-1β, IL-6, and TNF-α (p < 0.05) and higher activity of the GPR109A/NF-κB signaling pathway. A pretreatment of either pertussis toxin (PTX, inhibitor of GPR109A) or pyrrolidinedithiocarbamic (PDTC, inhibitor of NF-κB p65) was effective in preventing the elevated secretion of pro-inflammatory cytokines induced by low glucose plus BHB (p < 0.05). These results indicated that the low glucose plus BHB condition would induce an enhanced pro-inflammatory response through the activation of the GPR109A/NF-κB signaling pathway in primary yak AMs, which is probably the reason why yaks experience a higher rate of respiratory diseases and mortality. This study will offer new insight into the prevention and treatment of bovine respiratory diseases.

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β-Hydroxybutyrate Alleviates Low Glucose–Induced Apoptosis via Modulation of ROS-Mediated p38 MAPK Signaling

Cited by 2 publications

References 58 publications

Deconvoluting nitric oxide–protein interactions with spatially resolved multiplex imaging

Deconvoluting nitric oxide–protein interactions with spatially resolved multiplex imaging

Low Glucose plus β-Hydroxybutyrate Induces an Enhanced Inflammatory Response in Yak Alveolar Macrophages via Activating the GPR109A/NF-κB Signaling Pathway

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