β-ethoxyacrolein contamination increases malondialdehyde inhibition of milk xanthine oxidase activity

Cighetti, Giuliana; Debiasi, Sandra; Ciuffreda, Pierangela; Allevi, Pietro

doi:10.1016/s0891-5849(98)00155-5

Cited by 10 publications

(7 citation statements)

References 26 publications

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“…After the mixture was allowed to stand overnight at 7°C, the crystalline NaMDA was collected by filtration, washed with a small amount of acetone, and recrystallized twice from water-acetone. 1 16 The crystalline NaMDA was stored at −20°C under nitrogen.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

Behavior of Malondialdehyde in Oil-in-Water Emulsions

Vandemoortele

Meulenaer

2015

J. Agric. Food Chem.

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The impact of temperature, emulsifier, and protein type on the reactivity of malondialdehyde in oil-in-water emulsions was elucidated. Malondialdehyde recoveries in aqueous buffer, protein solutions, saturated oil, and fully hydrogenated coconut oil-in-water emulsions stabilized by whey proteins or Tween 20 at 4 or 40 °C were compared. At both temperatures, the reactivity of malondialdehyde in aqueous buffer was the same. In protein solutions, malondialdehyde concentrations were reduced further and its decrease was protein-dependent. Similar trends were found for emulsions. Surprisingly, malondialdehyde was very reactive in saturated oil because only 15% was recovered at 40 °C. However, the degradation in oil proved to be strongly temperature-dependent; at 4 °C, losses amounted to only 8%. This study revealed that malondialdehyde is a very reactive molecule, both in the presence and absence of proteins. Its use as a general oxidation marker should therefore be considered with care.

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Section: ■ Materials and Methodsmentioning

confidence: 99%

Behavior of Malondialdehyde in Oil-in-Water Emulsions

Vandemoortele

Meulenaer

2015

J. Agric. Food Chem.

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“…For total MDA evaluation serum (0·2 mL), spiked and analysed as described for free MDA, was hydrolysed in the presence of 1 mol L −1 NaOH for 60 min at 60 °C before the derivatization step. Pure MDA, crystallized as sodium salt [16] and d 2 ‐MDA were used for the calibration curves.…”

Section: Methodsmentioning

confidence: 99%

Oxidative status and malondialdehyde in β‐thalassaemia patients

Cighetti¹,

Duca

Bortone³

et al. 2002

Eur J Clin Investigation

132

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“…The purity assessment and the chemical identification of MDA and dMDA was carried out by NMR and GC-MS. The purification procedures and the use of malondialdehyde as the sodium salt are absolutely necessary as the crude MDA mixture, commonly used for biological studies, and deriving from the hydrolysis of 1,1,3,3-tetraethoxypropane, contains carbonyl compounds as by-products, and these might interfere with qualitative and quantitative MDA detection (6,31).…”

Section: Methodsmentioning

confidence: 99%

“…Standard stock solutions (10 M) were prepared by dissolving a weighed amount of MDA or dMDA in water and then storing them at Ϫ85°C. The real concentrations were confirmed at the beginning of each experiment by reading the absorbance at 267 nm (⑀ ϭ 3.18 ϫ 10 4 M Ϫ1 cm Ϫ1 ) (6,31).…”

Section: Methodsmentioning

confidence: 99%