β-Escin Effectively Modulates HUVECs Proliferation and Tube Formation

Abstract

In the present study we evaluated the anti-angiogenic activities of β-escin (the major active compound of Aesculus hippocastanum L. seeds). Human umbilical-vein endothelial cells (HUVECs) were used as an in vitro model for studying the molecular mechanism underlying the anti-angiogenic effect of β-escin. We investigated the in vitro effects on proliferation, migration, and tube formation of HUVECs and in vivo anti-angiogenic activity was evaluated in a chick chorioallantoic membrane (CAM) angiogenesis assay. Moreover, the effect on gene expressions was determined by the RT2 ProfilerTM human angiogenesis PCR Array. It was found that β-escin exerts inhibitory effect on the basic fibroblast growth factor (bFGF)-induced proliferation, migration and tube formation, as well as CAM angiogenesis in vivo. The inhibition of critical steps of angiogenic process observed with β-escin could be partially explained by suppression of Akt activation in response to bFGF. Moreover, the anti-angiogenic effects of β-escin could also be mediated via inhibition of EFNB2 and FGF-1 gene expressions in endothelial cells. In conclusion, β-escin affects endothelial cells as a negative mediator of angiogenesis in vitro and in vivo and may therefore be considered as a promising candidate for further research elucidating its underlying mechanism of action.

Gambogic amide inhibits angiogenesis by suppressing VEGF/VEGFR2 in endothelial cells in a TrkA-independent manner

“…The CAM assay was performed as described in a previous study (Varinská et al. 2018 ). Chicken eggs at seven days post-fertilization (Boehringer Ingelheim, Beijing) were cleaned with 70% ethanol, and a window of ∼1.5–2.0 cm 2 was gently opened on the blunt end of the egg without damaging the embryo.…”

ESCIN MITIGATES HYPOXIA MIMICKING NCI-H23 CELLS THROUGH MODULATION OF MMPs, HIF-1α AND HIF-2α

“…The effect of escin on the invasiveness of NCI-H23 cells was determined by Boyden chamber assay [26]. Escin treated NCI-H23 cells were seeded at the density of 1×10 5 Gelatin zymography in the collagen-coated upper chamber of the Boyden chamber (8 μm polycarbonate membrane). The bottom compartments of the Boyden chamber were filled with a 10% complete medium.…”

“…Escin is a saponin derived from the Aesculus hippocastanum. Several research studies have evidenced that anti-oxidant [2], anti-cancer [3], anti-inflammation [4], antiangiogenic properties [5], reverses multidrug resistance [6]. Escin has the ability to reduce MDA-MB-231, and KBM-5 cells invasion by regulation of invasive genes such as extracellular matrix (ECM) degradation enzyme matrix metalloproteinase-9 (MMP-9) [7][8][9].…”

Aesculus hippocastanum L. Extract Does Not Induce Fibroblast to Myofibroblast Conversion but Increases Extracellular Matrix Production In Vitro Leading to Increased Wound Tensile Strength in Rats

“…Qualitative and quantitative analysis of HCE sample was realized by HPLC-DAD-MS according to previously reported method [23]. Briefly, all HPLC analysis were carried out on the Agilent Technologies 1200 Series chromatograph (Agilent Technologies Deutschland, Walbron, Germany), equipped with a diode array spectrometer and MS ion trap detectors (AB SCIEX 3200 Q TRAP LC/MS/MS System, Applied Biosystems, Foster City, CA, USA).…”