β-cyclodextrin coating: improving biocompatibility of magnetic nanocomposites for biomedical applications

Abstract

The role Beta-cyclodextrin (βCD) on improving biocompatibility on healthy cellular and animal models was studied upon a formulation obtained from the development of a simple coating procedure. The obtained nanosystems were thoroughly characterized by FTIR, TGA, atomic absorption spectroscopy, dynamic light scattering and zeta potential, TEM/HR-TEM and magnetic properties. βCD might interact with the magnetic core through hosting OA. It is feasible that the nanocomposite is formed by nanoparticles of MG@OA dispersed in a βCD matrix. The evaluation of βCD role on biocompatibility was performed on two healthy models. To this end, in vivo studies were carried out on Caenorhabditis elegans. Locomotion and progeny were evaluated after exposure animals to MG, MG@OA, and MG@OA-βCD (10 to 500 µg/mL). The influence of βCD on cytotoxicity was explored in vitro on healthy rat aortic endothelial cells, avoiding alteration in the results derived from the use of transformed cell lines. Biological studies demonstrated that βCD attaching improves MG biocompatibility.

Hemolytic Response of Iron Oxide Magnetic Nanoparticles at the Interface and in Bulk: Extraction of Blood Cells by Magnetic Nanoparticles

“…Interestingly, the presence of α-Cyc-stabilized magnetic NPs only generates isotonic conditions. α-Cyc is highly water-soluble and non-surface-active oligosaccharide, which possesses excellent biocompatibility without causing any marked change in the environment of blood cells. , The adsorption of α-Cyc on crystalline magnetic NPs is expected to take place through non-covalent interactions where hydroxyl oxygen atoms of the toroid side play an important role. , Such a kind of adsorption leaves the α-Cyc cavity to accept a suitable guest molecule, and there are plenty of sugar residues of glycophorin, which constitute the outer layer of the blood cell membrane (glycocalyx predominantly made up of glycoprotein and glycolipid) and the lipid bilayer available to accomplish host–guest complexation. − This complexation does not disrupt the lipid bilayer because sugar residues provide necessary anchoring sites for α-Cyc-stabilized magnetic NPs; hence, no membrane disruption is observed. β-Cyc possesses slightly larger cavity size (∼6 Å) in comparison to that of α-Cyc (∼5 Å) and hence depicts similar hemolytic response and blood cell extraction behavior (Figure ).…”

1-Mesityl-3-(3-Sulfonatopropyl) Imidazolium Protects Against Oxidative Stress and Delays Proteotoxicity in C. elegans

“…To evaluate mobility, one animal (at the indicated days of adulthood, starting at day 0 (L4 stage)) was transferred to a multi-well plate containing 150 μl of M9 buffer (one worm/well). After 1 min of habituation, thrashing rates were manually counted under a dissecting scope as previously described ( Jones et al, 2011 ; Agotegaray et al, 2020 ). A single thrash is defined as a change in the direction of bending at the midbody ( Buckingham and Sattelle, 2009 ; Jones et al, 2011 ).…”

Drug discovery: Insights from the invertebrate Caenorhabditis elegans

“…Using invertebrate animal models in these early steps is ideal to reduce research costs and time as they help to identify compounds that, apart from being effective in interacting with the active target, maintain their efficacy even after absorption, distribution, metabolism, and excretion (ADME) processes. They also provide information about potential systemic toxicity and biocompatibility 96 …”

“…They also provide information about potential systemic toxicity and biocompatibility. 96 Although ADME processes are important sources of information compared with cell cultures, in C. elegans they have certain limitations that should be taken into account at the moment of processing results from drug-screening assays. In particular, the thick cuticle, which forms a strong barrier that limits drug absorption, can usually render false-negative results.…”