β-Cyclodextrin-Assisted Extraction of Polyphenols from Vine Shoot Cultivars

Rajha, Hiba N.; Chacar, Stéphanie; Afif, Charbel; Vorobiev, Eugène; Louka, Nicolas; Maroun, Richard G.

doi:10.1021/acs.jafc.5b00672

Cited by 50 publications

(28 citation statements)

References 21 publications

(32 reference statements)

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“…As the treatment time increased to 120 min, the recovered EGCG and ECG were declined to 91.1 mg/g and 35.6 mg/g, respectively. This might be due to the oxidation phenomenon that was likely to occur when subjecting phenolic compounds to high temperature for long durations (Palma and others ; Rajha and others ).…”

Section: Resultsmentioning

confidence: 99%

“…β‐CD has been generally recognized as safe by the Food and Drug Administration in 1998 and it is now extensively used as a flavor carrier and protectant in the food industry (Szente and Szejtli ). Recently, several researchers have reported that β‐CD can be used to extract polyphenols from grape pomace, apple pomace, vine shoot cultivars and Polygonum cuspidatum (Mantegna and others ; Ratnasooriya and Rupasinghe ; Rajha and others ; López‐Miranda and others ). Compared to organic solvents, β‐CD‐assisted extraction is more economic, safe, and green.…”

Section: Introductionmentioning

confidence: 99%

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Extraction of Epigallocatechin Gallate and Epicatechin Gallate from Tea Leaves Using β‐Cyclodextrin

Chen

Liu

et al. 2017

Journal of Food Science

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Use of organic solvents to extract phenolic compounds from plants may result in environmental pollution and cause health problems in persons. Replacing organic extraction solvents by green extracting agents without affecting the extraction yield is one of the most pressing problems to be solved. The aim of this study is to evaluate the capacity of β-cyclodextrin (β-CD) to recover phenolic compounds from tea leaves. The extract obtained using the ethanol/water mixture presented the highest total phenolic content, followed by those obtained using β-CD solution and water. HPLC analysis of the extracts showed that the addition of β-CD to the extracting agent had a selective effect on the extraction of epigallocatechin gallate (EGCG) and epicatechin gallate (ECG). The extraction yield of EGCG and ECG using 15 g/L β-CD were higher than that obtained using water and 50% ethanol. Molecular docking results indicated that the molecules of EGCG and ECG were more inclined to interact with β-CD than epigallocatechin, epicatechin, and gallocatechin. The impact of β-CD concentration, temperature, and time on EGCG and ECG extraction from tea leaves was investigated and the maximum amount of EGCG (118.7 mg/g) and ECG (54.6 mg/g) were achieved when extracted with 25 g/L aqueous β-CD solution at 60 °C for 60 min. The present study indicates that aqueous β-CD can be used as an alternative to organic solvents to recover EGCG and ECG from tea leaves.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Extraction of Epigallocatechin Gallate and Epicatechin Gallate from Tea Leaves Using β‐Cyclodextrin

Chen

Liu

et al. 2017

Journal of Food Science

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“…After an incubation of 30 min at room temperature, the absorbance was measured at 515 nm. The DPPH value of the extracts was expressed in micromolar Trolox equivalent per milliliter (μMTE; Rajha and others ).…”

Section: Methodsmentioning

confidence: 99%

The Impact of Long‐Term Intake of Phenolic Compounds‐Rich Grape Pomace on Rat Gut Microbiota

Chacar

Itani

Hajal

et al. 2017

Journal of Food Science

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The aim of this work is to evaluate the impact on the rat microbiota of long-term feeding with phenolic compounds (PC) rich grape pomace extracts. Thirty, 2-mo-old rats, were divided into 5 groups. Four groups were treated with different concentrations of PC (2.5, 5, 10, and 20 mg/kg/d diluted in 0.1% DMSO), and 1 group received 0.1% Dimethyl Sulfoxide (DMSO) alone (control group). The daily treatment lasted 14 mo. Major phenolic compounds constituents were characterized by the high-performance liquid chromatography and free radical scavenging capacity was measured by means of the DPPH assay. Fecal samples from young rats (2-mo old), and rats daily fed with PC or DMSO were collected at 6 and 14 mo posttreatment. The gut microbiota composition was analyzed by quantitative polymerase chain reaction. Bifidobacterium was significantly higher in the groups PC 2.5 and PC 5 than in control and young rats. Lactobacillus decreased with time in all treated and untreated groups. Bacteroides, Clostridium leptum subgroup (Clostridium cluster IV), and Enterococcus were not significantly changed by PC at any concentration when compared to control; nevertheless, after 14 mo of treatment all concentrations of PC abolished the increase of Clostridium sensu stricto (cluster I) (Clostridium Cluster I) observed in the control group when compared to young rats. PC do modulate selectively rat gut microbiome to a healthier phenotype in long-term feeding rats, and could counteract the adverse outcomes of aging on gut bacterial population.Keywords: aging, gut microbiota, phenolic compoundsPractical Application: This research shows that phenolic-rich grape pomace extracts exhibiting a high antioxidant activity, selectively modulate rat gut microbiota to a healthier phenotype within age in a long-term feeding rats.

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“…Aqueous solutions of β-CD have been successfully used to assist polyphenol extraction from various matrices, including pomegranate [30], tea leaves [31], vine shoots [32] and Polygonum cuspidatum [33], but combination of β-CD with DES has never been reported. In the view of clarifying whether such a combination could boost polyphenol extraction yield, extractions with the DES tested were also performed in the presence of 1.5% (w/v) β-CD.…”

Section: The Effect Of β-Cyclodextrin (β-Cd) Additionmentioning

confidence: 99%

Combination of Lactic Acid-Based Deep Eutectic Solvents (DES) with β-Cyclodextrin: Performance Screening Using Ultrasound-Assisted Extraction of Polyphenols from Selected Native Greek Medicinal Plants

et al. 2017

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Abstract:A series of novel L-lactic acid-based deep eutectic solvents (DES) were tested for polyphenol extraction performance, using organically grown, native Greek medicinal plants. The extractions were ultrasonically-assisted and the effect of the addition of β-cyclodextrin (β-CD) as extraction booster was also tested, at a concentration of 1.5% (w/v). The estimation of total polyphenol yield (Y TP ) suggested that DES composed of L-lactic acid and nicotinamide and L-lactic acid and L-alanine, both at a molar ratio of 7:1, are promising solvents giving significantly higher yields compared with 60% (v/v) aqueous ethanol and water. However, when β-CD was combined with DES comprised of L-lactic acid and ammonium acetate (molar ratio 7:1), the extraction yields obtained in some instances were equal of even higher. The pattern was not consistent when the yield in total flavonoids (Y TFn ) was considered, indicating water, 60% (v/v) aqueous ethanol and L-lactic acid:sodium acetate (molar ratio 7:1) to be the most efficient solvents. In this case, the effect of β-CD was of rather lower magnitude. The examination of the antioxidant activity of the extracts generated showed that there is a close correlation mainly with their concentration in total polyphenols.

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β-Cyclodextrin-Assisted Extraction of Polyphenols from Vine Shoot Cultivars

Cited by 50 publications

References 21 publications

Extraction of Epigallocatechin Gallate and Epicatechin Gallate from Tea Leaves Using β‐Cyclodextrin

Extraction of Epigallocatechin Gallate and Epicatechin Gallate from Tea Leaves Using β‐Cyclodextrin

The Impact of Long‐Term Intake of Phenolic Compounds‐Rich Grape Pomace on Rat Gut Microbiota

Combination of Lactic Acid-Based Deep Eutectic Solvents (DES) with β-Cyclodextrin: Performance Screening Using Ultrasound-Assisted Extraction of Polyphenols from Selected Native Greek Medicinal Plants

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