β-Chitin from the pens of Loligo sp.: Extraction and characterization

Lavall, Rodrigo L.; Assis, O. B. G.; Filho, Sérgio Paulo Campana

doi:10.1016/j.biortech.2006.09.002

Cited by 220 publications

(118 citation statements)

References 29 publications

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“…1). Lavall et al (2007) and Rinaudo (2006) also observed amide I band at 1656 cm -1 which is in agreement with the present study. NH-stretching peaks of standard chitin was at 3107 cm -1 and same band was also found in the extracted chitin at 3107 cm -1 .…”

Section: Resultssupporting

confidence: 93%

“…NH-stretching peaks of standard chitin was at 3107 cm -1 and same band was also found in the extracted chitin at 3107 cm -1 . Lavall et al (2007) in the extracted chitin compared with standard band stretching showed that extracted material was chitin and this material was further used for extraction of chitosan by deacetylation. Brugnerotto et al (2001) studied the FT-IR investigation of chitin and chitosan and showed the band spectrums of different bonds i.e.…”

Section: Resultsmentioning

confidence: 99%

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Extraction and characterization of chitin, chitosan and chitooligosaccharides from crab shell waste

Varun

Senani

Kumar

et al. 2017

IJAR

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An experiment was carried out to utilize the crab shell waste for the production of chitin by chemical extraction method. The obtained chitin was processed to make the more useful compound chitosan by deacetylation of chitin using strong NaOH solution at high temperature. Yield of chitin and chitosan from crab shell waste was found to be 12.2 % and 10.54 %, respectively. Chitosan was further used to produce chito-oligosaccharides (COS) by acid hydrolysis using 0.5 N H 2 SO 4 , 1N H 2 SO 4 and 7N HCl at hourly intervals upto 6 hours and the average yield was 0.55, 0.84 and 1.93 mg/ml, respectively. Chitin and chitosan were characterized by Fourier Transform Infrared spectroscopy (FT-IR) technique which depicts different band representing various bonds present in the molecule and which were in agreement with the bands of standard chitin and chitosan. Further, COS were formed after acid hydrolysis of chitosan, which was characterized by Thin Layer Chromatography (TLC) using glucosamine as a standard.

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Section: Resultssupporting

confidence: 93%

Section: Resultsmentioning

confidence: 99%

Extraction and characterization of chitin, chitosan and chitooligosaccharides from crab shell waste

Varun

Senani

Kumar

et al. 2017

IJAR

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“…These values are similar to those obtained for the chitin produced using B. subtilis fermentation [9] and chitin from shrimp Crangon crangon shells deproteinized by Alcalase® [10]. Compared with the commercial α-chitin [52], the two obtained chitins were more deacetylated, especially chitin prepared by chemical deproteinization. Regardless of the procedure of deproteinization employed, the 13 C CP/ MAS-NMR spectra exhibited the same main bands for the two prepared chitin.…”

Section: Cp/mas-nmr Spectroscopic Analysissupporting

confidence: 88%

Extraction and Characterization of Chitin, Chitosan, and Protein Hydrolysates Prepared from Shrimp Waste by Treatment with Crude Protease from Bacillus cereus SV1

Manni

Ghorbel-Bellaaj

Jellouli

et al. 2009

Appl Biochem Biotechnol

186

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Chitin is a polysaccharide found in abundance in the shell of crustaceans. In this study, the protease from Bacillus cereus SV1 was applied for chitin extraction from shrimp waste material of Metapenaeus monoceros. A high level of deproteinization 88.8% +/- 0.4 was recorded with an E/S ratio of 20. The demineralization was completely achieved within 6 h at room temperature in HCl 1.25 M, and the residual content of calcium in chitin was below 0.01%. (13)C CP/MAS-NMR spectral analysis of chitin prepared by the enzymatic deproteinization of shrimp wastes was found to be similar to that obtained by alkaline treatment and to the commercial alpha-chitin. The degree of N-acetylation, calculated from the spectrum, was 89.5%. Chitin obtained by treatment with crude protease from B. cereus was converted to chitosan by N-deacetylation, and the antibacterial activity of chitosan solution against different bacteria was investigated. Results showed that chitosan solution at 50 mg/mL markedly inhibited the growth of most Gram-negative and Gram-positive bacteria tested. Furthermore, the antioxidant potential of the protein hydrolysates obtained during enzymatic isolation of chitin was evaluated using various in vitro assays. All the samples exerted remarkable antioxidant activities. These results suggest that enzymatic deproteinization of the shrimp shell wastes, using B. cereus SV1 protease, could be applicable to the chitin production process.

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“…The average weight of each gladius collected from the squid, T. pacificus was 0.345 ± 0.082 g. Almost, 42-43 % water insoluble white fibrous β-chitin without any pigment was obtained after demineralisation and deprotenization treatments [29]. Approximately, 0.078 g of β-chitin was obtained from a gladius and the amount of chitin obtained in this study was comparable with the reported result [5,8,30].…”

Section: Characterization Of Gcspssupporting

confidence: 66%

Effective immobilization of glucose oxidase on chitosan submicron particles from gladius of Todarodes pacificus for glucose sensing

Anusha¹,

Fleming²,

Kim

et al. 2015

Bioelectrochemistry

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β-Chitin from the pens of Loligo sp.: Extraction and characterization

Cited by 220 publications

References 29 publications

Extraction and characterization of chitin, chitosan and chitooligosaccharides from crab shell waste

Extraction and characterization of chitin, chitosan and chitooligosaccharides from crab shell waste

Extraction and Characterization of Chitin, Chitosan, and Protein Hydrolysates Prepared from Shrimp Waste by Treatment with Crude Protease from Bacillus cereus SV1

Effective immobilization of glucose oxidase on chitosan submicron particles from gladius of Todarodes pacificus for glucose sensing

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