β-Catenin and Rho GTPases as downstream targets of TGF-β1 during pulp repair

Shao, Meiying; Cheng, Ran; Wang, Feng‐Ming; Yang, Hui; Cheng, Li; Hu, Tao

doi:10.1042/cbi20100114

Cited by 10 publications

(7 citation statements)

References 45 publications

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“…As an example, the literature shows that Rho and ROCK participate with the β-catenin and TGF-β signaling pathways, even though the TGF-β or Wnt proteins are absent [35]–[36]. In other cases ROCK acts downstream when Wnt and TGF-β are present [37]. Therefore, we suggest that ROCK has the ability to serve as an intermediate between these pathways, possibly controlling this balance between active/inactive downstream states (Figure 5L).…”

Section: Discussionmentioning

confidence: 83%

Inhibition of Rho Kinase Regulates Specification of Early Differentiation Events in P19 Embryonal Carcinoma Stem Cells

et al. 2011

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BackgroundThe Rho kinase pathway plays a key role in many early cell/tissue determination events that take place in embryogenesis. Rho and its downstream effector Rho kinase (ROCK) play pivotal roles in cell migration, apoptosis (membrane blebbing), cell proliferation/cell cycle, cell-cell adhesion and gene regulation. We and others have previously demonstrated that inhibition of ROCK blocks endoderm differentiation in embryonal carcinoma stem cells, however, the effect of ROCK inhibition on mesoderm and ectoderm specification has not been fully examined. In this study, the role of ROCK within the specification and differentiation of all three germ layers was examined.Methodology/Principal FindingsP19 cells were treated with the specific ROCK inhibitor Y-27623, and increase in differentiation efficiency into neuro-ectodermal and mesodermal lineages was observed. However, as expected a dramatic decrease in early endodermal markers was observed when ROCK was inhibited. Interestingly, within these ROCK-inhibited RA treated cultures, increased levels of mesodermal or ectodermal markers were not observed, instead it was found that the pluripotent markers SSEA-1 and Oct-4 remained up-regulated similar to that seen in undifferentiated cultures. Using standard and widely accepted methods for reproducible P19 differentiation into all three germ layers, an enhancement of mesoderm and ectoderm differentiation with a concurrent loss of endoderm lineage specification was observed with Y-27632 treatment. Evidence would suggest that this effect is in part mediated through TGF-β and SMAD signaling as ROCK-inhibited cells displayed aberrant SMAD activation and did not return to a ‘ground’ state after the inhibition had been removed.Conclusions/SignificanceGiven this data and the fact that only a partial rescue of normal differentiation capacity occurred when ROCK inhibition was alleviated, the effect of ROCK inhibition on the differentiation capacity of pluripotent cell populations should be further examined to elucidate the role of the Rho-ROCK pathway in early cellular ‘fate’ decision making processes.

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Section: Discussionmentioning

confidence: 83%

Inhibition of Rho Kinase Regulates Specification of Early Differentiation Events in P19 Embryonal Carcinoma Stem Cells

et al. 2011

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“…For example, previous studies have shown that TGF-␤1 is a central factor released in high amounts during pulp repair, regulating migration of DPSCs toward the injured site [49] and subsequent differentiation into odontoblastic cells producing reparative dentin [50]. On the other hand, ␤-catenin has been found to mediate the downstream events of TGF␤1 signaling, by its translocation into the nucleus and interaction with LEF/TCF to regulate gene expression during pulp repair [18]. In another study it was also shown that pulp cavity preparation procedures directly activate ␤-catenin accumulation in pulp cells beneath the cavity [51].…”

Section: Discussionmentioning

confidence: 96%

“…During tooth initiation and morphogenesis, Wnt3, Wnt7b, Wnt10a and Wnt10b, in conjunction with sonic hedgehog (SHH), regulate cell proliferation, migration and differentiation [16,17]. Importantly, TGF-␤1 has been shown to interact with Wnt/␤-catenin signaling to induce various biological effects during pulp repair [18].…”

Section: Introductionmentioning

confidence: 99%

Wnt/β-catenin signaling regulates Dental Pulp Stem Cells’ responses to pulp injury by resinous monomers

et al. 2015

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“…So far, only a few reports give emphasis on the area for pathways serving functions in pulp repair. [ 33 ]…”

Section: Dentine and Pulp And β Cateninmentioning

confidence: 99%

β catenin in health: A review

Prakash

Swaminathan

2015

J Oral Maxillofac Pathol

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β catenin belongs to the armadillo family of proteins. It plays a crucial role in developmental and homeostatic processes. Wnts are a family of 19 secreted glycoproteins that transduce multiple signaling cascades, including the canonical Wnt/β catenin pathway, Wnt/Ca2+ pathway and the Wnt/polarity pathway. This is a review on β catenin, Wnt proteins and their secretion, the signaling pathway, the associated factors and the crucial role of β catenin in odontogenesis.

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β-Catenin and Rho GTPases as downstream targets of TGF-β1 during pulp repair

Cited by 10 publications

References 45 publications

Inhibition of Rho Kinase Regulates Specification of Early Differentiation Events in P19 Embryonal Carcinoma Stem Cells

Inhibition of Rho Kinase Regulates Specification of Early Differentiation Events in P19 Embryonal Carcinoma Stem Cells

Wnt/β-catenin signaling regulates Dental Pulp Stem Cells’ responses to pulp injury by resinous monomers

β catenin in health: A review

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