β‐Carotene—production methods, biosynthesis from <i>Phaffia rhodozyma</i>, factors affecting its production during fermentation, pharmacological properties: A review

Mujeeb, Mohd.; Nashvia, Syeda; Prasad, B. Bhanu; Mujeeb, Mohd

doi:10.1002/bab.2301

Cited by 16 publications

(10 citation statements)

References 112 publications

(189 reference statements)

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“…β-Carotene, with high added value, is usually obtained by chemical synthesis, plant extraction, and microbial fermentation. 9 The β-carotene produced by microbial fermentation has a high biological activity and is not season restricted. 10 Y. lipolytica has garnered much interest for the heterologous synthesis of β-carotene.…”

Section: Introductionmentioning

confidence: 99%

Concomitant Production of Erythritol and β-Carotene by Engineered Yarrowia lipolytica

Zhang

et al. 2023

J. Agric. Food Chem.

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While the expansion of the erythritol production industry has resulted in unprecedented production of yeast cells, it also suffers from a lack of effective utilization. β-Carotene is a value-added compound that can be synthesized by engineered Yarrowia lipolytica. Here, we first evaluated the production performance of erythritol-producing yeast strains under two different morphologies and then successfully constructed a chassis with yeast-like morphology by deleting Mhy1 and Cla4 genes. Subsequently, β-carotene synthesis pathway genes, CarRA and CarB from Blakeslea trispora, were introduced to construct the βcarotene and erythritol coproducing Y. lipolytica strain ylmcc. The rate-limiting genes GGS1 and tHMG1 were overexpressed to increase the β-carotene yield by 45.32-fold compared with the strain ylmcc. However, increased β-carotene accumulation led to prolonged fermentation time; therefore, transporter engineering through overexpression of YTH1 and YTH3 genes was used to alleviate fermentation delays. Using batch fermentation in a 3 L bioreactor, this engineered Y. lipolytica strain produced erythritol with production, yield, and productivity values of 171 g/L, 0.56 g/g glucose, and 2.38 g/(L•h), respectively, with a concomitant βcarotene yield of 47.36 ± 0.06 mg/g DCW. The approach presented here improves the value of erythritol-producing cells and offers a low-cost technique to obtain hydrophobic terpenoids.

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Section: Introductionmentioning

confidence: 99%

Concomitant Production of Erythritol and β-Carotene by Engineered Yarrowia lipolytica

Zhang

et al. 2023

J. Agric. Food Chem.

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“…They can be found in vegetables and in fruits, in which orange carrots are the most common source of β‐carotene. As said above, industrial carotenoids are produced by extraction and chemical synthesis; chemical synthesis produces hazardous wastes, which are harmful to the environment (Gupta et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Pareto optimal metabolic engineering for the growth‐coupled overproduction of sustainable chemicals

Amaradio

Ojha

Jansen

et al. 2022

Biotech & Bioengineering

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Our research aims to help industrial biotechnology develop a sustainable economy using green technology based on microorganisms and synthetic biology through two case studies that improve metabolic capacity in yeast models Yarrowia lipolytica (Y. lipolytica) and Saccharomyces cerevisiae (S. cerevisiae). We aim to increase the production capacity of beta-carotene (β-carotene) and succinic acid, which are among the highest market demands due to their versatile use in numerous consumer products. We performed simulations to identify in silico ranking of strains based on multiple objectives: the growth rate of yeast microorganisms, the number of used chromosomes, and the production capability of β-carotene (for Y. lipolytica) and succinate (for S. cerevisiae). Our multiobjective optimization methodology identified notable gene deletions by searching a vast solution space to highlight near-optimal strains on Pareto Fronts, balancing the above-cited three objectives. Moreover, preserving the metabolic constraints and the essential genes, this study produced robust results: seven significant strains of Y. lipolytica and seven strains of S. cerevisiae. We examined gene knockout to study the function of genes and pathways. In fact, by studying the frequently silenced genes, we found that when the GPH1 gene is knocked out in S. cerevisiae, the isocitrate lyase enzyme is activated, which converts the isocitrate into succinate. Our goals are to simplify and facilitate the in vitro processes. Hence, we present strains with the least possible number of knockout genes and solutions in which the genes are turned off on the same chromosome. Therefore, we present results where the constraints mentioned above are met, like the strains where only two genes are switched off and other strains where half of the knockout genes are on the same chromosome. This study offers solutions for developing an efficient in vitro mutagenesis for microorganisms and demonstrates the efficiency of multiobjective optimization in automatizing metabolic engineering processes.

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“…Carotenoids have been proven as potent antioxidant and anticancer agents, in addition, many of them including zeaxanthin and lutein, protect the eyes from cataracts and macular degeneration (Jaswir et al 2011 ; Mrowicka et al 2022 ). Due to their biological characteristics and possible advantages for human health, many carotenoids are currently employed as natural colorants for food, dietary supplements and nutraceuticals for pharmacological and aesthetic applications (Ashokkumar et al 2022 ; Guleria et al 2017 ; Gupta et al 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Sustainable production of bacterioruberin carotenoid and its derivatives from Arthrobacter agilis NP20 on whey-based medium: optimization and product characterization

Noby

Khattab

Soliman

2023

Bioresour. Bioprocess.

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Bacterioruberin and its rare glycosylated derivatives are produced by Arthrobacter agilis as an adaptation strategy to low temperature conditions. The high antioxidant properties of bacterioruberin held great promise for different future applications like the pharmaceutical and food industries. Microbial production of bacterioruberin via a cost-effective medium will help increase its commercial availability and industrial use. The presented study aims to optimize the production of the rare C50 carotenoid bacterioruberin and its derivatives from the psychotrophic bacteria Arthrobacter agilis NP20 strain on a whey-based medium as a cost effective and readily available nutritious substrate. The aim of the study is extended to assess the efficiency of whey treatment in terms of estimating total nitrogen content in treated and untreated whey samples. The significance of medium ingredients on process outcome was first tested individually; then the most promising factors were further optimized using Box Behnken design (BBD). The produced carotenoids were characterized using UV–visible spectroscopy, FTIR spectroscopy, HPLC–DAD chromatography and HPLC-APCI-MS spectrometry. The maximum pigment yield (5.13 mg/L) was achieved after a 72-h incubation period on a core medium composed of 96% sweet whey supplemented with 0.46% MgSO4 & 0.5% yeast extract and inoculated with 6% (v/v) of a 24 h pre-culture (109 CFU/mL). The cost of the formulated medium was 1.58 $/L compared with 30.1 $/L of Bacto marine broth medium. The extracted carotenoids were identified as bacterioruberin, bis-anhydrobacteriouberin, mono anhydrobacterioruberin, and glycosylated bacterioruberin. The presented work illustrates the possibility of producing bacterioruberin carotenoid from Arthrobacter agilis through a cost-effective and eco-friendly approach using cheese whey-based medium. Graphical Abstract

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β‐Carotene—production methods, biosynthesis from Phaffia rhodozyma, factors affecting its production during fermentation, pharmacological properties: A review

Cited by 16 publications

References 112 publications

Concomitant Production of Erythritol and β-Carotene by Engineered Yarrowia lipolytica

Concomitant Production of Erythritol and β-Carotene by Engineered Yarrowia lipolytica

Pareto optimal metabolic engineering for the growth‐coupled overproduction of sustainable chemicals

Sustainable production of bacterioruberin carotenoid and its derivatives from Arthrobacter agilis NP20 on whey-based medium: optimization and product characterization

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