β-Carotene Inhibits Expression of Matrix Metalloproteinase-10 and Invasion in Helicobacter pylori-Infected Gastric Epithelial Cells

Bae, Suji; Lim, Joo Weon; Kim, Hye Young

doi:10.3390/molecules26061567

Cited by 23 publications

(18 citation statements)

References 55 publications

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“…MMP-10 is an important member of MMPs. MMP-10 was anomalously expressed in numerous tumors, such as gastric cancer [ 11 ], esophageal cancer [ 12 ], and head and neck cancer [ 13 ]. MMP-10 is a protease closely related to tumor invasion and metastasis, whose main function is to degrade extracellular matrix.…”

Section: Discussionmentioning

confidence: 99%

Identification of Key Genes in Nasopharyngeal Carcinoma Based on Bioinformatics Analysis

Song

Feng

Cao

et al. 2022

Computational Intelligence and Neuroscience

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Objective. This study aimed to identify key genes associated with the pathogenesis of nasopharyngeal carcinoma (NPC) by bioinformatics analysis. Methods. Datasets (GSE13597 and GSE34573) were screened and downloaded from the comprehensive gene expression database (GEO). GEO2R online tool was adopted to analyze microarray data GSE13597 and GSE34573 related to NPC. Volcano plot was generated using Bioconductor in R software. “Pheatmap” was used to draw heatmaps based on the top 10 regulated genes of GSE13597 and GSE34573. GO and KEGG analyses were conducted via online tool DAVID. We uploaded the DEGs of NPC to STRING software and then used Cytoscape software to draw PPI network of DEGs. Results. 216 DEGs were obtained in GSE13597 between patient and control group (111 up-regulated DEGs and 105 down-regulated DEGs). 1101 DEGs were obtained in GSE34573 (470 up-regulated DEGs and 641 down-regulated DEGs). 63 common differential genes were screened named co-DEGs in the two datasets. These DEGs were mainly associated with defense response to bacterium, cell-matrix adhesion, chemokine-mediated signaling pathway, tissue homeostasis, humoral immune response, cilium movement, cilium organization, cilium assembly, and epithelial cilium movement. KEGG pathway enrichment analysis showed that DEGs were mainly involved in viral protein interaction with cytokine and cytokine receptor, salivary secretion, p53 signaling pathway, IL-17 signaling pathway, cell cycle, PI3K-Akt signaling pathway, and ECM-receptor interaction. We identified seven hub genes, including FN1, MMP-10, MUC1, KIF23, CDK1, MUC5B, and MUC5AC. Conclusions. Seven hub genes, including FN1, MMP-10, MUC1, KIF23, CDK1, MUC5B, and MUC5AC, might be therapeutic potential biomarkers of NPC.

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Section: Discussionmentioning

confidence: 99%

Identification of Key Genes in Nasopharyngeal Carcinoma Based on Bioinformatics Analysis

Song

Feng

Cao

et al. 2022

Computational Intelligence and Neuroscience

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“…In most cases, ROS affects cell signal transduction and causes gastric cancer. erefore, eliminating ROS levels in H. pylori-infected cells may prevent worsening the disease [23]. Our functional experiments revealed that CN reduced the production of ROS in GSE-1 cells caused by H. pylori infection in a concentration-dependent manner.…”

Section: Discussionmentioning

confidence: 66%

The Anti-Inflammatory Effect of Callicarpa nudiflora Extract on H. Pylori-Infected GES-1 Cells through the Inhibition of ROS/NLRP3/Caspase-1/IL-1β Signaling Axis

Chai

et al. 2022

Canadian Journal of Infectious Diseases and Medical Microbiolog

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Helicobacter pylori (H. pylori) is the main pathogenic factor of gastric cancer, chronic gastritis, and other gastric diseases. It has been found that Callicarpa nudiflora (CN) as an air-dried leaf extract has a broad-spectrum antibacterial effect. This study aims to examine the effect of CN on H. pylori-infected GES-1 cells in vitro and elucidate its underlying mechanism by extracting active ingredients from air-dried leaves. GES-1 cells were cocultured with HPSS1 at MOI = 100 : 1 and treated with different concentrations of CN (100 and 200 μg/ml). Results showed that CN can significantly reduce cellular LDH leakage and attenuate H. pylori-induced cell apoptosis and ROS production in GSE-1 cells, so as to protect gastric epithelial cells from damage by H. pylori. CN can also inhibit the secretion of inflammatory factors, such as TNF-α, IL-1β, IL-6, and IL-8. After CN treatment, the expression levels of active caspase-1, PYCARD, and NLRP3 were remarkably decreased in the treatment groups compared with the model group. To sum up, CN is highly protective against H. pylori-induced cell damage and apoptosis; CN can depress NLRP3 inflammasome activation and ROS production via the ROS/NLRP3/caspase-1/IL-1β signaling axis to suppress H. pylori-triggered inflammatory response and pyroptosis.

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“…In our study, we found that MMP10 was activated under conditions of oxidative stress in the RPE. In this regard, it has been shown that reducing intracellular oxidative stress, both by transfecting cells with a physiological redox regulator [ 34 ] and by adding an antioxidant [ 35 ], β-Carotene, reduces MMP10 expression in cells. Based on these findings, it can be speculated that MMP10 may have either a beneficial role in facilitating the recycling of the BM in situations where its deterioration increases cellular stress or a deleterious role in facilitating the sprouting vessels to cross the membrane.…”

Section: Discussionmentioning

confidence: 99%

Matrix Metalloproteinase 10 Contributes to Choroidal Neovascularisation

et al. 2022

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Age-related macular degeneration (AMD) is currently the main cause of severe visual loss among older adults in developed countries. The pathophysiology has not been clarified, but oxidative stress is believed to play a major role. Matrix metalloproteinases (MMP) may play a prominent role in several steps of the pathophysiology of AMD, especially in its neovascular form; therefore, there is of great interest in understanding their role in choroidal neovascularisation. This study aimed to elucidate the role of MMP10 in the development of choroidal neovascularisation (CNV). We have demonstrated that MMP10 was expressed by retinal pigment epithelium cells and endothelial cells of the neovascular membrane, in cell culture, mouse and human retina. MMP10 expression and activity increased under oxidative stress conditions in ARPE-19 cells. MMP10-/- mice developed smaller laser-induced areas of CNV. Furthermore, to exclude a systemic MMP10 imbalance in these patients, plasma MMP10 concentrations were assessed in an age- and sex-matched sample of 52 control patients and 52 patients with neovascular AMD and no significant differences were found between the groups, demonstrating that MMP10 induction is a local phenomenon. Our findings suggest that MMP10 participates in the development of choroidal neovascularisation and promotes MMP10 as a possible new therapeutic target.

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β-Carotene Inhibits Expression of Matrix Metalloproteinase-10 and Invasion in Helicobacter pylori-Infected Gastric Epithelial Cells

Cited by 23 publications

References 55 publications

Identification of Key Genes in Nasopharyngeal Carcinoma Based on Bioinformatics Analysis

Identification of Key Genes in Nasopharyngeal Carcinoma Based on Bioinformatics Analysis

The Anti-Inflammatory Effect of Callicarpa nudiflora Extract on H. Pylori-Infected GES-1 Cells through the Inhibition of ROS/NLRP3/Caspase-1/IL-1β Signaling Axis

Matrix Metalloproteinase 10 Contributes to Choroidal Neovascularisation

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