β-carotene genetically-enriched lyophilized orange juice increases antioxidant capacity and reduces β-amyloid proteotoxicity and fat accumulation in Caenorhabditis elegans

Paulo, Iolanda Raquel Ferreira; Caland, Ricardo Basílio de Oliveira; Cadavid, Cesar Orlando Muñoz; Melo, Giovanna Martins; Bezerra, Liliane Soares De Castro; Pons, Elsa; Peña, Leandro; Oliveira, Riva de Paula

doi:10.1016/j.fochms.2022.100141

Cited by 6 publications

(4 citation statements)

References 50 publications

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“…Other possible mechanisms involved in the reduction of C. elegans paralysis by DS extract might be the regulation of different transcription factors. For instance, Citrus sinensis extracts enriched in carotenoids have shown to alleviate the paralysis induced by Aβ toxicity 28 , 29 . In these studies, the authors demonstrated that orange juices up-regulate the expression of detoxification and antioxidant response genes ( gcs-1 , gst-4 , sod-3 , hsp-4 or hsp-16.2 ) by acting through the transcription factor SKN-1, but also delaying the Aβ-amyloid-induced paralysis in a manner requiring SKN-1, DAF-16, and HSF-1 transcription factors.…”

Section: Discussionmentioning

confidence: 99%

“…The unique and complex chemical composition of D. salina makes this microalga a potential source of neuroprotective compounds that could interfere with one or more AD hallmarks. Carotenoids have shown to protect the cellular components against ROS 14 , enhance the endogenous antioxidant systems 17 , and modulate inflammation-related mechanisms 15 , and these effects have been demonstrated also in vivo using C. elegans models [24][25][26][27][28][29] . Apart from carotenoids and their antioxidant capacity, transgenic C. elegans strains have also been used to evaluate the protection of different natural extracts against the toxicity of Aβ plaques formation 52 .…”

Section: Discussionmentioning

confidence: 99%

“…It has also been widely employed in neurosciencerelated studies of AD 20 , including therapy test with drugs and natural extracts [21][22][23] . For instance, the antioxidant potential of individual carotenoids (some of which are present in D. salina), such as astaxanthin 24 , lutein 25 , β-carotene, lycopene and β-cryptoxanthin 26 , or food matrices enriched in carotenoids, such as orange juices [27][28][29] , have been demonstrated on C. elegans.…”

Section: Introductionmentioning

confidence: 99%

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In vivo neuroprotective capacity of a Dunaliella salina extract - comprehensive transcriptomics and metabolomics study

Valdés,

Sánchez-Martínez,

Gallego

et al. 2024

npj Sci Food

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In this study, an exhaustive chemical characterization of a Dunaliella salina (DS) microalga extract obtained using supercritical fluids has been performed, and its neuroprotective capacity has been evaluated in vivo using an Alzheimer’s disease (AD) transgenic model of Caenorhabditis elegans (strain CL4176). More than 350 compounds were annotated in the studied DS extract, with triacylglycerols, free fatty acids (FAs), carotenoids, apocarotenoids and glycerol being the most abundant. DS extract significantly protects C. elegans in a dose-dependent manner against Aβ-peptide paralysis toxicity, after 32 h, 53% of treated worms at 50 µg/mL were not paralyzed. This concentration was selected to further evaluate the transcriptomics and metabolomics changes after 26 h by using advanced analytical methodologies. The RNA-Seq data showed an alteration of 150 genes, mainly related to the stress and detoxification responses, and the retinol and lipid metabolism. The comprehensive metabolomics and lipidomics analyses allowed the identification of 793 intracellular metabolites, of which 69 were significantly altered compared to non-treated control animals. Among them, different unsaturated FAs, lysophosphatidylethanolamines, nucleosides, dipeptides and modified amino acids that have been previously reported as beneficial during AD progression, were assigned. These compounds could explain the neuroprotective capacity observed, thus, providing with new evidences of the protection mechanisms of this promising extract.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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In vivo neuroprotective capacity of a Dunaliella salina extract - comprehensive transcriptomics and metabolomics study

Valdés,

Sánchez-Martínez,

Gallego

et al. 2024

npj Sci Food

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show abstract

“…In vivo studies in model organisms have directly demonstrated that carotenoids protect against ageing. Several studies used C. elegans and showed that supplementation with carotenoid-rich extracts from plant sources not only protect against oxidative stress but also increase lifespan [20,[24][25][26][27][28][29]. Moreover, individual carotenoids improve health in C. elegans.…”

Section: Mechanisms By Which Phytoene Impacts Ageingmentioning

confidence: 99%

Phytoene and Phytoene-Rich Microalgae Extracts Extend Lifespan in C. elegans and Protect against Amyloid-β Toxicity in an Alzheimer’s Disease Model

Morón-Ortiz,

Karamalegos,

Mapelli-Brahm

et al. 2024

Preprint

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The role of foods is shifting from focusing on the provision of energy and basic nutrients to also include bioactive compounds that prevent the development of chronic age-related diseases. Mi-croalgae offer a source of nutritional compounds with important health effects, and have the po-tential for production of health-promoting products, without increasing agricultural land use and negatively impacting the environment. Here we investigate the health effects of microalgal ex-tracts with high levels of the colourless carotenoid phytoene. Phytoene is widely available from dietary sources and can be detected across different tissues in the human body. However, it is usually regarded as a precursor for the synthesis of other carotenoids, rather than a bioactive molecule. We utilised the model organism C. elegans to show that phytoene-rich extracts from Chlorella sorokiniana and Dunaliella bardawil have anti-ageing properties. The extracts protect against oxidative damage and amyloid-β42 proteotoxicity (a major pathology of Alzheimer's disease), and extends lifespan. We show that pure phytoene also has these anti-ageing effects, suggesting that phytoene is a bioactive molecule with positive effects on ageing and longevity.

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Omics approaches to investigate the neuroprotective capacity of a Citrus sinensis (sweet orange) extract in a Caenorhabditis elegans Alzheimer’s model

Sánchez-Martínez

Álvarez-Rivera

Valdés

2023

Food Research International

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β-carotene genetically-enriched lyophilized orange juice increases antioxidant capacity and reduces β-amyloid proteotoxicity and fat accumulation in Caenorhabditis elegans

Cited by 6 publications

References 50 publications

In vivo neuroprotective capacity of a Dunaliella salina extract - comprehensive transcriptomics and metabolomics study

In vivo neuroprotective capacity of a Dunaliella salina extract - comprehensive transcriptomics and metabolomics study

Phytoene and Phytoene-Rich Microalgae Extracts Extend Lifespan in C. elegans and Protect against Amyloid-β Toxicity in an Alzheimer’s Disease Model

Omics approaches to investigate the neuroprotective capacity of a Citrus sinensis (sweet orange) extract in a Caenorhabditis elegans Alzheimer’s model

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