β-adrenergic receptor signaling regulates <i>Ptgs2</i> by driving circadian gene expression in osteoblasts

Hanabusa, Takao; Tanaka, Kenjiro; Togari, Akifumi

doi:10.1242/jcs.148148

Cited by 27 publications

(30 citation statements)

References 35 publications

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“…This result suggests that regulation via the E-box site generates the oscillation in the Ptgs2 transcription. In addition to its regulation via E-box, Ptgs2 transcription is regulated by Nfil3 via the D-box site (22) and Rev-erb␣ via RORE, as we have described in the present study. The amplitude and phase in the rhythmic expression of Ptgs2 may be balanced by these regulations.…”

Section: Discussionsupporting

confidence: 64%

“…Bmal1 control of Ptgs2 transcription has been suggested in other tissues (7,22,25,38). Moreover, our previous data obtained from a DNA microarray in UESCs isolated from day 4.5 of rat pregnancy (49) suggested that the expression level of the Ptgs2 transcript is rhythmic.…”

Section: Discussionmentioning

confidence: 90%

“…A recent study showed that the Ptgs2 protein is gradually expressed in the rat endometrium in response to deciduogenic stimuli (14). More recently, several reports have indicated that the transcription of the Ptgs2 gene is controlled by the cellular circadian clock (7,22,25,56).A growing number of studies have provided evidence that numerous peripheral circadian clock systems are partially selfoperative and independent in their responses to external and internal stimuli other than the stimuli originating from the suprachiasmatic nucleus, known as the central pacemaker of the circadian clock (17,30,55). Circadian clock systems generate cellular rhythms in physiological functions via identified transcriptional and posttranscriptional regulatory processes, which comprise a core group of clock genes and their protein products.…”

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confidence: 99%

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confidence: 99%

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Removal of Rev-erbα inhibition contributes to the prostaglandin G/H synthase 2 expression in rat endometrial stromal cells

Isayama

Zhao

Chen

et al. 2015

American Journal of Physiology-Endocrinology and Metabolism

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The rhythmic expression of clock genes in the uterus is attenuated during decidualization. This study focused on Ptgs2, which is essential for decidualization, as a putative clock-controlled gene, and aimed to reveal the functions of clock genes in relation to Ptgs2 during decidualization. We compared the transcript levels of clock genes in the rat uterus on days 4.5 (D4.5) and 6.5 of pregnancy. The transcript levels of clock genes (Per2, Bmal1, Rorα, and Rev-erbα) had decreased at implantation sites on day 6.5 (D6.5e) compared with those on D4.5, whereas Ptgs2 transcripts had increased on D6.5e. Similar observations of Rev-erbα and Ptgs2 were also obtained in the endometrium on D6.5e by immunohistochemistry. In the decidual cells induced by medroxyprogesterone and 2-O-dibutyryl-cAMP, the rhythmic expression levels of clock genes were attenuated, whereas Ptgs2 transcription was induced. These results indicate that decidualization causes the attenuation of clock genes and the induction of Ptgs2. Furthermore, in the experiment of Bmal1 siRNA, the rhythmic expression of clock genes and Ptgs2 was attenuated by the siRNA. Transcript levels of Ptgs2 and prostaglandin (PG)E₂ production were increased by treatment with the Rev-erbα antagonist, suggesting the contribution of the nuclear receptor Rev-erbα to Ptgs2 expression. Moreover, Rev-erbα knockdown enhanced the induction of Ptgs2 transcription and PGE₂ production by forskolin. Chromatin immunoprecipitation-PCR analysis revealed that Rev-erbα could directly bind to a proximal RORE site of Ptgs2. Collectively, this study demonstrates that the attenuation of the circadian clock, especially its core component Rev-erbα, contributes to the induction of Ptgs2 during decidualization.

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Section: Discussionsupporting

confidence: 64%

Section: Discussionmentioning

confidence: 90%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Removal of Rev-erbα inhibition contributes to the prostaglandin G/H synthase 2 expression in rat endometrial stromal cells

Isayama

Zhao

Chen

et al. 2015

American Journal of Physiology-Endocrinology and Metabolism

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“…When cells reached confluency, medium supplemented with 10 mM β-glycerophosphate and 10 mg/ml ascorbic acid was used. Media were changed every 2-4 days (Hirai et al, 2014a).…”

Section: Bone Histomorphometrymentioning

confidence: 99%

Modulation of osteoblast differentiation and bone mass by 5-HT2A receptor signaling in mice

Tanaka

Hanabusa

Ishibashi

et al. 2015

European Journal of Pharmacology

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Crosstalk between Bone and Nerves within Bone

et al. 2021

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For the past two decades, the function of intrabony nerves on bone has been a subject of intense research, while the function of bone on intrabony nerves is still hidden in the corner. In the present review, the possible crosstalk between bone and intrabony peripheral nerves will be comprehensively analyzed. Peripheral nerves participate in bone development and repair via a host of signals generated through the secretion of neurotransmitters, neuropeptides, axon guidance factors and neurotrophins, with additional contribution from nerve‐resident cells. In return, bone contributes to this microenvironmental rendezvous by housing the nerves within its internal milieu to provide mechanical support and a protective shelf. A large ensemble of chemical, mechanical, and electrical cues works in harmony with bone marrow stromal cells in the regulation of intrabony nerves. The crosstalk between bone and nerves is not limited to the physiological state, but also involved in various bone diseases including osteoporosis, osteoarthritis, heterotopic ossification, psychological stress‐related bone abnormalities, and bone related tumors. This crosstalk may be harnessed in the design of tissue engineering scaffolds for repair of bone defects or be targeted for treatment of diseases related to bone and peripheral nerves.

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β-adrenergic receptor signaling regulates Ptgs2 by driving circadian gene expression in osteoblasts

Cited by 27 publications

References 35 publications

Removal of Rev-erbα inhibition contributes to the prostaglandin G/H synthase 2 expression in rat endometrial stromal cells

Removal of Rev-erbα inhibition contributes to the prostaglandin G/H synthase 2 expression in rat endometrial stromal cells

Modulation of osteoblast differentiation and bone mass by 5-HT2A receptor signaling in mice

Crosstalk between Bone and Nerves within Bone

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