β-Actin is a useful internal control for tissue-specific gene expression studies using quantitative real-time PCR in the half-smooth tongue sole Cynoglossus semilaevis challenged with LPS or Vibrio anguillarum

Li, Zhaojie; Yang, Lijun; Wang, Jing; Shi, Wenchun; Pawar, R. A.; Liu, Yumin; Xu, Chao; Cong, Weihong; Hu, Qiaoru; Lu, Ting; Xia, Fan Di; Guo, Wei; Zhao, Meng; Zhang, Yuanyuan

doi:10.1016/j.fsi.2010.02.021

Cited by 103 publications

(51 citation statements)

References 18 publications

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“…It was one of the first RNAs to be used as an internal standard for qRT-PCR since its transcription level remains relatively constant in response to experimental manipulation in most tissues (Li et al, 2010).…”

Section: Primers For Pcr Gapdh (Target Gene)mentioning

confidence: 99%

Potential use of GAPDH m-RNA in estimating PMI in brain tissue of albino rats at different environmental conditions

El‐Ghamry

Mohamed

Hassan

et al. 2017

Egypt J Forensic Sci

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Background: Estimation of the postmortem interval (PMI) is a critical issue in forensic science. Various approaches have been used to determine the PMI including physical, biochemical and entomological methods. Most of these methods have practical limitations or provide insufficient results in certain conditions. Postmortem degradation of RNA may be a useful tool for PMI estimation if there is a correlation between the quantity of residual RNA and the elapsed time. This study aimed to evaluate the use of GAPDH mRNA quantity in the brain as a possible indicator for PMI in different environmental conditions. Methods: Seventy-eight adult female albino rats were sacrificed by cervical dislocation. Rats were divided into five groups, the control group and 4 studied groups left after sacrificing in different conditions (ambient air at 30°C and at 6°C, buried in sand and submerged under water). Brain samples were obtained at different intervals (0, 24, 48 and 96 h postmortem). The mRNA of GAPDH gene of rats' brain was quantitatively detected by qRT-PCR.

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Section: Primers For Pcr Gapdh (Target Gene)mentioning

confidence: 99%

Potential use of GAPDH m-RNA in estimating PMI in brain tissue of albino rats at different environmental conditions

El‐Ghamry

Mohamed

Hassan

et al. 2017

Egypt J Forensic Sci

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“…The results identified by NormFinder were in line with the prediction obtained with geNorm, thus, B2M and 18S were exhibited as the most stably housekeeping genes under normal physiological condition. As reported in many other studies, under different experimental conditions, different tissues and different growth stage, the most stably housekeeping gene is different (Filby and Tyler 2007, Fernandes et al 2008, Infante et al 2008, Zhong et al 2008, Bower and Johnston 2009, Li et al 2010, Rverg4rd et al 2010, Dang and Sun 2011, Lrvoll et al 2011, Zheng and Sun 2011. For example, in red drum under conditions of bacterial infection, RPS35, ACTB, EF1A, ND1, TUBB, EF1A, ACTB and ACTB were analyzed as the ideal internal references in brain, gill, heart, intestine, kidney, liver, muscle and spleen, respectively (Sun and Hu 2015).…”

Section: Discussionmentioning

confidence: 57%

“…In recent years, golden pompano industries developed rapidly and it became the main species of cage culture. Along with the expansion, T. ovatus aquaculture industries worldwide have suffered viral diseases outbreaks what caused serious economic losses (Xu et al 2010, Su et al 2015. Recently, qRT-PCR has become a widely used method of investigating gene expression in golden pompano.…”

mentioning

confidence: 99%

Selection of reference genes for quantitative real-time RT-PCR on gene expression in Golden Pompano (Trachinotus ovatus)

Chen¹,

Zhang²,

Huang³

et al. 2017

Polish Journal of Veterinary Sciences

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Golden pompano (Trachinotus ovatus) is an important economically fish species. In this study, with an aim to identify reliable reference genes for quantitative real-time PCR (qRT-PCR) in golden pompano, we evaluated the expression stability of eight housekeeping genes in the presence and absence of poly I:C stimulation in eight tissues. The PCR data was analyzed by geNorm and NormFinder algorithms. The results showed that the expression of all the examined genes exhibited tissue-dependent variations. When under normal physiological condition, geNorm and NormFinder identified B2M and 18S as suitable genes. When studying gene expression under conditions of poly

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“…In the current study, GAPDH was used as a reference gene due to the fact that its transcription level remains relatively constant in response to experimental manipulation in most tissues (Li et al, 2010). The same study instrument was adopted by other researches such as Bauer et al (2008) and Hass et al (2009), wherein GAPDH was detected in all body fluids including menstrual and peripheral blood.…”

Section: Discussionmentioning

confidence: 99%

Differential genes expression biomarkers for menstrual and peripheral blood stains analysis

Moawad

Zaghlol

Abdelsalam

et al. 2018

Egypt J Forensic Sci

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Background: Identification of menstrual blood is an important issue in forensic biology, but there are no confirmatory methods for its detection. This study aimed to investigate the use of mRNA markers (hemoglobin alpha (HBA), matrix metalloproteinases 7 &11 (MMP7 &MMP11) as forensic markers for differentiation between menstrual and peripheral blood stains. Results: HBA was present in all peripheral and menstrual blood samples with higher levels in peripheral blood. MMP 7&11 were absent in peripheral blood samples. MMP7 levels were higher than that of MMP11 throughout days of menstruation (days 1-5). Conclusion: HBA, MMP7&MMP11 could be useful markers for differentiation between menstrual and peripheral blood stains. Further studies should be conducted on various fabrics and other body fluids.

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β-Actin is a useful internal control for tissue-specific gene expression studies using quantitative real-time PCR in the half-smooth tongue sole Cynoglossus semilaevis challenged with LPS or Vibrio anguillarum

Cited by 103 publications

References 18 publications

Potential use of GAPDH m-RNA in estimating PMI in brain tissue of albino rats at different environmental conditions

Potential use of GAPDH m-RNA in estimating PMI in brain tissue of albino rats at different environmental conditions

Selection of reference genes for quantitative real-time RT-PCR on gene expression in Golden Pompano (Trachinotus ovatus)

Differential genes expression biomarkers for menstrual and peripheral blood stains analysis

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