β-1,6-Glucan From Pleurotus eryngii Modulates the Immunity and Gut Microbiota

Wang, Xue; Qu, Yunhe; Wang, Yuan; Wang, Xiang; Xu, Jialei; Zhao, Hailing; Zheng, Donglin; Sun, Lin; Tai, Guihua; Zhou, Yifa; Cheng, Hairong

doi:10.3389/fimmu.2022.859923

Cited by 17 publications

(19 citation statements)

References 53 publications

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“…This β-1,6-branched β-1,3-glucan is common in various Pleurotus species, such as P. albidus [ 52 ], P. eryngii [ 53 ], P. ostreatus [ 54 ], P. sajor-caju [ 47 ], among others. Nonetheless, many more β-glucans have been described from other Pleurotus mushrooms, such as linear β-1,3-glucans [ 46 , 48 ] and linear β-1,6-glucans [ 55 ], but also β-1,3/1,6-glucans with α-linked-1,6 substitutions [ 56 ].…”

Section: Resultsmentioning

confidence: 99%

Transglycosylation of Stevioside by a Commercial β-Glucanase with Fungal Extracted β-Glucans as Donors

Zerva

Mohammadi

Dimopoulos

et al. 2023

Waste Biomass Valor

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Alternative sweeteners, such as steviol glucosides from the plant Stevia rebaudiana Bertoni, are becoming increasingly popular for the design of next-generation foodstuffs. However, the bitter aftertaste of native steviol glucosides is one of the main reasons behind consumer reluctance towards stevia-containing products. Biocatalysis could be a sustainable solution to this problem, through addition of glucosyl moieties to the molecule. Glycoside hydrolases are enzymes performing transglycosylation reactions, and they can be exploited for such modifications. In the present work, the commercial β-glucanase Finizym 250L® was employed for the transglycosylation of stevioside. After optimization of several reaction parameters, the maximal reaction yield obtained was 19%, with barley β-glucan as the glycosyl donor. With the aim to develop a sustainable process, β-glucan extracts from different fungal sources were prepared. Pulsed Electric Field pretreatment of mycelial biomass resulted in extracts with higher β-glucan content. The extracts were tested as alternative glucosyl donors, reaching up to 15.5% conversion yield, from Pleurotus-extracted β-glucan. Overall, in the present work a novel enzymatic process for the modification of stevioside is proposed, with concomitant valorization of β-glucans extracted from fungal biomass, potentially generated as a byproduct from other applications, in concert with the principles of circular economy. Graphical Abstract

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Section: Resultsmentioning

confidence: 99%

Transglycosylation of Stevioside by a Commercial β-Glucanase with Fungal Extracted β-Glucans as Donors

Zerva

Mohammadi

Dimopoulos

et al. 2023

Waste Biomass Valor

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“…The DCs of CD8 + T and CD56 + NK cells are activated, enhancing the cross-activation of adaptive and innate immune responses [ 32 ]. Polysaccharides from Pleurotus eryngii with a branch of β-1,6-glucan increase the proportion of CD4 T and CD8 T cells to improve immunity [ 33 ]. According to our results, PAPS1 not only enhanced the splenic expressions of CD4, CD8 and CD56 in immunosuppressed mice, but it also increased the IgA and IgM serum levels.…”

Section: Discussionmentioning

confidence: 99%

The Structural Characterization and Immunomodulatory Activity of Polysaccharides from Pleurotus abieticola Fruiting Bodies

et al. 2022

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Polysaccharides obtained from mushrooms have been reported to possess immunomodulatory properties. In this study, a water-soluble polysaccharide was purified from the fruiting bodies of Pleurotus abieticola, entitled PAPS1. After its composition and structural analysis, the immunomodulatory activity was investigated in immunosuppressed mice induced by cyclophosphamide (CTX) at a dosage of 70 mg/kg by intraperitoneal injection for 7 days. After 28 days of intragastric administration, PAPS1 alleviated cyclophosphamide (CTX)-induced histopathological damage and increased the expressions of splenic CD4, CD8, CD56 and IgM in the serums of immunosuppressed mice. PAPS1 suppressed the oxidative stress indicated by preventing the increases in ROS and MDA levels. According to the intestinal microflora analysis, PAPS1 regulated 11 bacteria at the gene level, including Helicobacter and Paraprevotella, which are related to immunity and oxidative capacity. Compared with CTX-treated mice, significant increases in immune-related cytokines, such as interleukin (IL)-2, IL-6 and IL-12 in the serums of mice treated with PAPS1, were observed. Finally, PAPS1 can strongly increase the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and its downstream proteins. In conclusion, PAPS1-boosted immunity may be related to its suppression on oxidative stress via enhancing the activity of Nrf2 signaling. Thus, PAPS1 can be investigated as a candidate for immunomodulatory therapy.

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“…Cultivated oyster mushrooms of the genus Pleurotus are interesting as a source of biologically active glucans with a variety of bioactivities, with potential use in the field of biomedical sciences [17]. In particular, many of the biological activities of P. eryngii, such as immunostimulating, antitumor, hepatoprotective, antioxidant, hypolipidemic and hypoglycemic, are attributable to polysaccharides [9,13,14,[18][19][20][21][22]. β-glucans from Pleurotus sp.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant Activity of Polysaccharides from the Edible Mushroom Pleurotus eryngii

et al. 2023

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In this study the antioxidant and neuroprotective activity of an enriched polysaccharide fraction (EPF) obtained from the fruiting body of cultivated P. eryngii was evaluated. Proximate composition (moisture, proteins, fat, carbohydrates and ash) was determined using the AOAC procedures. The EPF was extracted by using, in sequence, hot water and alkaline extractions followed by deproteinization and precipitation with cold ethanol. Total α- and β-glucans were quantified using the Megazyme International Kit. The results showed that this procedure allows a high yield of polysaccharides with a higher content of (1-3; 1-6)-β-D-glucans. The antioxidant activity of EPF was detected from the total reducing power, DPPH, superoxide, hydroxyl and nitric oxide radical scavenging activities. The EPF was found to scavenge DPPH, superoxide, hydroxyl and nitric oxide radicals with a IC50 values of 0.52 ± 0.02, 1.15 ± 0.09, 0.89 ± 0.04 and 2.83 ± 0.16 mg/mL, respectively. As assessed by the MTT assay, the EPF was biocompatible for DI-TNC1 cells in the range of 0.006–1 mg/mL and, at concentrations ranging from 0.05 to 0.2 mg/mL, significantly counteracted H2O2-induced reactive oxygen species production. This study demonstrated that polysaccharides extracted from P. eryngii might be used as functional food to potentiate the antioxidant defenses and to reduce oxidative stress.

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β-1,6-Glucan From Pleurotus eryngii Modulates the Immunity and Gut Microbiota

Cited by 17 publications

References 53 publications

Transglycosylation of Stevioside by a Commercial β-Glucanase with Fungal Extracted β-Glucans as Donors

Transglycosylation of Stevioside by a Commercial β-Glucanase with Fungal Extracted β-Glucans as Donors

The Structural Characterization and Immunomodulatory Activity of Polysaccharides from Pleurotus abieticola Fruiting Bodies

Antioxidant Activity of Polysaccharides from the Edible Mushroom Pleurotus eryngii

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