α6β4 Integrin, a Master Regulator of Expression of Integrins in Human Keratinocytes

Abstract

Background: Keratinocyte migration involves the coordinated expression of various integrin heterodimers. Results: Loss of ␣6␤4 integrin expression impairs cell migration and decreases ␣2 and ␣3 integrin subunit expression via transcriptional and translational mechanisms. Conclusion: Migration of human keratinocytes requires ␣6␤4 integrin-dependent regulation of integrin subunit expression. Significance: ␣6␤4 integrin controls integrin expression profiles and thereby regulates migration.

Laminin-332 and α3β1 Integrin–Supported Migration of Bronchial Epithelial Cells Is Modulated by Fibronectin

“…For these and subsequent studies, we focused on BEP2D cells, as we could generate clonal populations exhibiting a knockdown in expression of the LM332 receptor a6b4 integrin ( Figure 7A). Consistent with our recent findings in human keratinocytes (20), knockdown of a6 integrin expression in BEP2D cells led to a decrease in b4 integrin surface expression ( Figure 7B). However, in contrast to our previous findings in keratinocytes, loss of a6b4 integrin expression in BEP2D cells did not affect surface expression of a3 integrin ( Figure 7B).…”

“…LM332 matrix supports cell adhesion and migration in an a6b4 and/or a3b1 integrin-dependent manner (13,(20)(21)(22)(23)(24)(25). Because our results indicate that LM332 promotes bronchial epithelial cell migration, we next wished to assess which integrin receptor is responsible for regulating such motility.…”

“…Human keratinocytes migrate on LM332-rich matrix (13,20). In contrast, fibronectin in the matrix of mouse keratinocytes appears to inhibit LM332-mediated skin cell motility (18).…”

Transcriptional Analysis of Left-sided Colitis, Pancolitis, and Ulcerative Colitis-associated Dysplasia

“…[33][34][35] In addition to their role in stable adhesion, these proteins are also important in cell migration and have consequently been found upregulated during wound healing and tumorigenesis. [35][36][37] The lower LAMC2, ITGA6, and EZR gene expressions in UC-associated dysplasia as described in cohort 1 have, to our knowledge, not been described previously.…”

Integrins in Wound Healing

“…108 In addition, it may regulate the expression of a 2 b 1 and a 3 b 1 integrins in keratinocytes. 109 Integrins of the b 1 family are essential for wound re-epithelialization. The expression of b 1 integrins is strongly upregulated after wounding in the wound-edge keratinocytes and in several suprabasal keratinocyte layers, and they are critical for proper re-epithelialization (Fig.…”