α6β1 Integrin is a potential clinical marker for evaluating sperm quality in men

Reddy, Venkata Rami K; Rajeev, Shrimali K; Gupta, Vijayalakshmi

doi:10.1016/s0015-0282(03)00368-6

Cited by 26 publications

(16 citation statements)

References 27 publications

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“…This interpretation is supported mainly by the fact that the integrin subunits that correspond to the Ln receptor, particularly subunits a6 and b1, are expressed in human sperm. These subunits are located in the plasma membrane of the acrosomal region and in the equatorial segment [10,49]. The results of the immunoneutralization test offers additional support that the effect of Ln is specific.…”

Section: Discussionmentioning

confidence: 77%

The Laminin-Induced Acrosome Reaction in Human Sperm Is Mediated by Src Kinases and the Proteasome1

Tapia

Rojas

Morales

et al. 2011

Biology of Reproduction

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The aim of this work was to determine whether laminin (Ln), an extracellular matrix protein, induces the intracellular events that may be involved in producing the acrosome reaction in human sperm. To this end, we evaluated the effect of Ln on tyrosine phosphorylation, intracellular calcium concentration, proteasome activity, and phosphorylation in human sperm. Aliquots of highly motile sperm selected with a Percoll gradient, were incubated with different concentrations of Ln (0-20 μg/ml) for different periods (0-18 h). The percentage of viable acrosome-reacted sperm was evaluated using fluorescein isothiocyanate-labeled Pisum sativum agglutinin and Hoechst 33258 DNA dye. Tyrosine phosphorylation was evaluated by Western blot analysis. The chymotrypsin-like activity of the proteasome was evaluated with a fluorogenic peptide, and intracellular calcium concentration was measured with fura-2. The results indicate that Ln stimulated the acrosome reaction of human sperm in a dose-dependent manner. This increase was drastically inhibited in the presence of herbimycin A, SU6656, and epoxomicin. In addition, Ln increased proteasome activity and phosphorylation; both events were inhibited by herbimycin A and SU6656. Finally, Ln induced an increase in intracellular calcium concentration, which was inhibited by SU6656 and epoxomicin. These results suggest that Ln is able to induce the acrosome reaction. This effect may be mediated by Src kinase and the proteasome, with the consequent induction of a calcium influx.

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Section: Discussionmentioning

confidence: 77%

The Laminin-Induced Acrosome Reaction in Human Sperm Is Mediated by Src Kinases and the Proteasome1

Tapia

Rojas

Morales

et al. 2011

Biology of Reproduction

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“…When entering the periviteline space, OPN attached to the post-equatorial segment would mediate the interaction of sperm with the oolema, also through integrins and/or CD44. Integrins (αv and α5) have been identified in the bovine (Erikson et al, 2008) and human spermatozoa (Fusi et al, 1996;Reddy et al, 2003), as well as on human oolema (D'Cruz, 1996). The CD44 transmembrane glycoproteins are present in bovine sperm and oocyte.…”

Section: Proteins Involved In Acrosome Reaction and Fertilizationmentioning

confidence: 99%

Functional aspects of seminal plasma and sperm proteins and their potential as molecularmarkers of fertility

Moura

Memili

2016

Anim Reprod

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Molecular components of sperm and in the media surrounding them influence bull fertility. Given this concept, proteins of the seminal plasma modulate crucial functions and events of reproduction, such as sperm motility and capacitation, cell protection, acrosome reaction, fertilization and embryonic development. Sperm proteins are also important for successful fertilization, egg activation and embryo development. Empirical associations between seminal and sperm proteins and fertility in the bovine indicate that these proteins are potential molecular markers of the male reproductive status.

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“…In Xenopus, integrin 1 is present on the oocyte membrane throughout oogenesis and during maturation it is localized in several membrane vesicles in the cytoplasm might be to provide the material source for the rapid membrane formation during cleavage (Muller et al 1993). Even integrin 61 might serve as potential clinical marker for evaluating sperm quality in men (Reddy et al 2003).…”

Section: Integrin β1mentioning

confidence: 99%

Phospho-Signaling at Oocyte Maturation and Fertilization: Set Up for Embryogenesis and Beyond Part II. Kinase Regulators and Substrates

Hasan¹,

Mutoh²,

Kihira³

et al. 2012

Embryogenesis

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α6β1 Integrin is a potential clinical marker for evaluating sperm quality in men

Cited by 26 publications

References 27 publications

The Laminin-Induced Acrosome Reaction in Human Sperm Is Mediated by Src Kinases and the Proteasome1

The Laminin-Induced Acrosome Reaction in Human Sperm Is Mediated by Src Kinases and the Proteasome1

Functional aspects of seminal plasma and sperm proteins and their potential as molecularmarkers of fertility

Phospho-Signaling at Oocyte Maturation and Fertilization: Set Up for Embryogenesis and Beyond Part II. Kinase Regulators and Substrates

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