α6β1 integrin directs migration of neuronal precursors in adult mouse forebrain

Emsley, Jason G.; Hagg, Theo

doi:10.1016/s0014-4886(03)00209-7

Cited by 96 publications

(73 citation statements)

References 73 publications

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“…Our previously published data (Graus-Porta et al, 2001;Blaess et al, 2004) provide strong evidence that many of the migratory events that are important for the development of the cerebral and cerebellar cortex progress normally in the absence of ␤1 integrins. Similarly, although studies with function blocking antibodies have suggested that ␤1 integrins control cell motility of neuroblasts in the RMS, for example by controlling leading process extension (Murase and Horwitz, 2002;Emsley and Hagg, 2003), our data now show that ␤1-deficient neuroblasts in the RMS still elaborate extensive leading processes and are able to migrate directionally toward the OB. However, the cells migrate with reduced speed, suggesting that integrins control some aspects of migration.…”

Section: ␤1 Integrins and Cell Migration In The Cnssupporting

confidence: 51%

“…One study has reported that ␤1 integrins are expressed in the RMS until postnatal day 10 (P10) and that antibodies to ␤1 slow down migration before but not after P10 (Murase and Horwitz, 2002). A second study has provided evidence that the integrin ␣6␤1 is expressed even in the adult RMS and that injection of ␤1 antibodies disrupts the adult RMS (Emsley and Hagg, 2003). In both studies, it has remained unclear whether the antibodies affected the migration of neuroblasts directly or indirectly through effects on other cells or other aspects of cell behavior such as proliferation and differentiation.…”

Section: Introductionmentioning

confidence: 99%

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β1 Integrins Control the Formation of Cell Chains in the Adult Rostral Migratory Stream

Belvindrah¹,

Hankel²,

Walker³

et al. 2007

J. Neurosci.

152

126

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The subventricular zone (SVZ) of the lateral ventricle is the major site of neurogenesis in the adult brain. Neuroblasts that are born in the SVZ migrate as chains along the rostral migratory stream (RMS) to the olfactory bulb. Little is known about the mechanisms that control interactions between neuroblasts during their migration. Here we show that migrating neuroblasts express ␤1integrins and that the integrin ligand laminin is localized to cell chains. Using genetically modified mice and time-lapse video recordings of SVZ explants, we demonstrate that ␤1 integrins and laminin promote the formation of cell chains. Laminin also induces the aggregation of purified neuroblasts. We conclude that the formation of cell chains in the RMS is controlled in part by ␤1 integrins via binding to laminin. In addition, we provide evidence that ␤1 class integrins are required for the maintenance of the glial tubes and that defects in the glial tubes lead to the ectopic migration of neuroblasts into the surrounding tissue.

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Section: ␤1 Integrins and Cell Migration In The Cnssupporting

confidence: 51%

Section: Introductionmentioning

confidence: 99%

β1 Integrins Control the Formation of Cell Chains in the Adult Rostral Migratory Stream

Belvindrah¹,

Hankel²,

Walker³

et al. 2007

J. Neurosci.

152

126

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“…Close association among NPCs or between NPCs and glial cells within the RMS is believed to be important to maintain chain migration, as disruption to the cell-cell interactions disrupts NPC chain migration. Several classes of adhesion and guidance molecules, such as integrins and laminin, have been identified, which are important for NPC chain migration [3,54,55]. Furthermore, these molecules signal through downstream Rho/ROCK effectors [56][57][58][59].…”

Section: Regulation Of Npc Morphology and Migration By Rho Gtpase Sigmentioning

confidence: 99%

The Rho Kinase Pathway Regulates Mouse Adult Neural Precursor Cell Migration

et al. 2011

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Adult neural precursor cells (NPCs) in the subventricular zone (SVZ) normally migrate via the rostral migratory stream (RMS) to the olfactory bulb (OB). Following neural injury, they also migrate to the site of damage. This study investigated the role of Rho-dependent kinase (ROCK) on the migration of NPCs in vitro and in vivo. In vitro, using neurospheres or SVZ explants, inhibition of ROCK using Y27632 promoted cell body elongation, process protrusion, and migration, while inhibiting NPC chain formation. It had no effect on proliferation, apoptosis, or differentiation. Both isoforms of ROCK were involved. Using siRNA, knockdown of both ROCK1 and ROCK2 was required to promote NPC migration and morphological changes; knockdown of ROCK2 alone was partially effective, with little/no effect of knockdown of ROCK1 alone. In vivo, infusion of Y27632 plus Bromodeoxyuridine (BrdU) into the lateral ventricle for 1 week reduced the number of BrdU-labeled NPCs in the OB compared with BrdU infusion alone. However, ROCK inhibition did not affect the tangential-to-radial switch of NPC migration, as labeled cells were present in all OB layers. The decrease in NPC number at the OB was not attributed to a decrease in NPCs at the SVZ. However, ROCK inhibition decreased the density of BrdU-labeled cells in the RMS and increased the distribution of these cells to ectopic brain regions, such as the accessory olfactory nucleus, where the majority differentiated into neurons. These findings suggest that ROCK signaling regulates NPC migration via regulation of cell-cell contact and chain migration.

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“…In the niche, the state of each cell [i.e., cell cycle, apoptosis, and cell-cell or cell-extra cellular matrix (ECM) interactions] is strictly regulated to maintain stem cell homeostasis. Although ECM proteins are enriched in the SVZ (24,49,50), the niche for NSCs, the physiological significance of their carbohydrate structures has not been well characterized. In general, lectins exert their biological effects by binding to certain carbohydrate structures.…”

Section: Galectin-1-null Mice Have Fewer Neural Progenitor Cells In Tmentioning

confidence: 99%

A carbohydrate-binding protein, Galectin-1, promotes proliferation of adult neural stem cells

Sakaguchi

Shingo

Shimazaki³

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

143

150

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In the subventricular zone of the adult mammalian forebrain, neural stem cells (NSCs) reside and proliferate to generate young neurons. We screened factors that promoted the proliferation of NSCs in vitro by a recently developed proteomics technique, the ProteinChip system. In this screen, we identified a soluble carbohydrate-binding protein, Galectin-1, as a candidate. We show herein that Galectin-1 is expressed in a subset of slowly dividing subventricular zone astrocytes, which includes the NSCs. Based on results from intraventricular infusion experiments and phenotypic analyses of knockout mice, we demonstrate that Galectin-1 is an endogenous factor that promotes the proliferation of NSCs in the adult brain.lectin ͉ mobilization ͉ stem cell niche

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α6β1 integrin directs migration of neuronal precursors in adult mouse forebrain

Cited by 96 publications

References 73 publications

β1 Integrins Control the Formation of Cell Chains in the Adult Rostral Migratory Stream

β1 Integrins Control the Formation of Cell Chains in the Adult Rostral Migratory Stream

The Rho Kinase Pathway Regulates Mouse Adult Neural Precursor Cell Migration

A carbohydrate-binding protein, Galectin-1, promotes proliferation of adult neural stem cells

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