α2HS-glycoprotein, an Antagonist of Transforming Growth Factor β <b>
                     <i>In vivo</i>
                  </b>, Inhibits Intestinal Tumor Progression

Swallow, Carol J.; Partridge, Emily A.; Macmillan, Jennifer C.; Tajirian, Tania; DiGuglielmo, Gianni M.; Hay, K. M.; Szweras, Melanie; Jahnen‐Dechent, Willi; Wrana, Jeff; Redston, Mark; Gallinger, Steven; Dennis, James W.

doi:10.1158/0008-5472.can-04-1117

Cited by 89 publications

(80 citation statements)

References 47 publications

(57 reference statements)

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“…This is an important indicator that AHSG is involved in the insulin signaling pathway. Furthermore, it appears to boost the likelihood of developing gestational diabetes during pregnancy (Srinivas et al, 1993;Kalabay et al, 2002;Swallow et al, 2004;Stefan et al, 2006;Briana et al, 2008). In fact, our results show that serum AHSG levels were increased in the PE group as determined by both the SRM assay ( Figure 3) and ELISA assay (Figure 4).…”

Section: Resultsmentioning

confidence: 61%

Discovery of the serum biomarker proteins in severe preeclampsia by proteomic analysis

et al. 2011

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Preeclapsia (PE) is a severe disorder that occurs during pregnancy, leading to maternal and fetal morbidity and mortality. PE affects about 3-8% of all pregnancies. In this study, we conducted liquid chromatographymass spectrometry/mass spectrometry (LC-MS/MS) to analyze serum samples depleted of the six most abundant proteins from normal and PE-affected pregnancies to profile serum proteins. A total of 237 proteins were confidently identified with ＜ 1% false discovery rate from the two groups of duplicate analysis. The expression levels of those identified proteins were compared semiquantitatively by spectral counting. To further validate the candidate proteins with a quantitative mass spectrometric method, selective reaction monitoring (SRM) and enzyme linked immune assay (ELISA) of serum samples collected from pregnant women with severe PE (n = 8) or normal pregnant women (n = 5) was conducted. α2-HS-glycoprotein (AHSG), retinol binding protein 4 (RBP4) and α-1-microglobulin/bikunin (AMBP) and Insulin like growth factor binding protein, acid labile subunit (IGFBP-ALS) were confirmed to be differentially expressed in PE using SRM (P ＜ 0.05). Among these proteins, AHSG was verified by ELISA and showed a statistically significant increase in PE samples when compared to controls.

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Section: Resultsmentioning

confidence: 61%

Discovery of the serum biomarker proteins in severe preeclampsia by proteomic analysis

et al. 2011

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“…Alpha-2-HS glycoprotein (AHSG), is a glycoprotein present in the serum, is synthesised by hepatocytes and is important in blocking transforming growth factor-beta1 signal transduction, which is associated with tumour progression. AHSG has been observed to be depleted in certain tumours compared with normal tissue [19,20]. Kawakami et al [21] reported that AHSG was differentially expressed in the sera of hepatocellular carcinoma (HCC) patients who had undergone curative radiofrequency ablation treatment and may be a candidate biomarker for the development of diagnostic and therapeutic tools.…”

Section: Discussionmentioning

confidence: 99%

2‐D difference gel electrophoresis of the lung squamous cell carcinoma versus normal sera demonstrates consistent alterations in the levels of ten specific proteins

et al. 2007

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Research Article 2-D difference gel electrophoresis of the lung squamous cell carcinoma versus normal sera demonstrates consistent alterations in the levels of ten specific proteinsMost lung cancers are diagnosed too late for curative treatment to be possible, therefore early detection is crucial. Serum proteins are a rich source of biomarkers and have the potential to be used as diagnostic and prognostic indicators for lung cancer. In order to examine differences in serum levels of specific proteins associated with human lung squamous carcinoma, immunodepletion of albumin and five other high-abundant serum proteins followed by 2-D difference gel electrophoresis (DIGE) analysis and subsequent MS was used to generate a panel of proteins found to be differentially expressed between the cancer and normal samples. Proteins found to have increased abundance levels in squamous cell carcinoma sera compared to normal sera included apolipoprotein A-IV precursor, chain F; human complement component C3c, haptoglobin, serum amyloid A protein precursor and Ras-related protein Rab-7b. Proteins found to have lower abundance levels in squamous cell carcinoma sera compared to normal sera included alpha-2-HS glycoprotein, hemopexin precursor, proapolipoprotein, antithrombin III and SP40; 40. The data presented here demonstrate that high-abundant protein removal combined with 2-D DIGE is a powerful strategy for the discovery of potential biomarkers. The identification of lung cancer-specific biomarkers is crucial to early detection, which in turn could lead to a dramatic increase in survival rates. IntroductionOver 1 million people are diagnosed with lung cancer each year [1]. Most lung cancer is diagnosed too late for curative treatment to be possible. Efforts at early detection and treatment have had little success to date and hence the overall prognosis remains poor. In the majority of those diagnosed with lung cancer, the disease has already metastasised at the time of diagnosis. Lung cancer comprises broadly of two groups, small cell lung carcinoma (SCLC) and non-small cell lung carcinoma (NSCLC). NSCLC comprises more than 80% of lung cancers including squamous cell carcinoma, adenocarcinoma and large cell carcinoma, with squamous carcinoma being the most common type.A major factor in the high mortality of lung cancer patients is the presence of metastatic tumours in approximately two-thirds of patients at time of diagnosis [2]. Detection of cancer in these patients at earlier stages would increase survival rates dramatically. The identification of lung cancer-specific biomarkers is critical to early detection. The pathophysiology of lung squamous cell carcinoma development is complex and incompletely understood. Genetic alterations involved in the pathogenesis of lung cancer produce proteins involved in cell growth, invasion/ metastasis, differentiation, cell cycle processes, apoptosis and angiogenesis. Discovering these mechanisms and pathways will undoubtedly lead to new ways in dealing with prevention, early detection an...

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“…23,24 Fetuin-A has been shown to block TGF-␤1 binding to the extracellular motifs of TGF-␤II receptor, suppressing TGF-␤ signaling and inhibiting TGF-␤ induced epithelial-mesenchymal transition. 4 TGF-␤ inhibits normal epithelial cell proliferation, 25 but in many human cancers, TGF-␤ expression is elevated. 26 To determine whether fetuin-A modulates TGF-␤ signaling during mammary carcinogenesis, we probed mammary tissue sections of PyMT/Fet Ϫ/Ϫ and PyMT/Fet ϩ/ϩ mice with antiphospho-Smad2/3 (P-Smad2/3), a downstream target of TGF-␤ as a readout.…”

Section: Fetuin-a Attenuates Tgf-␤ Signaling In Normal Mammary Epithementioning

confidence: 99%

“…[1][2][3][4] We previously demonstrated that it is required for optimal in vitro and in vivo growth of Lewis lung carcinoma cells, and our studies suggested that it influences tumor growth via phosphatidylinositol 3 (PI3)-kinase/Akt signaling. 3 It is necessary to elucidate whether or not it plays a crucial role in the growth of both normal and tumor cells not only in culture (where it is a major growth supplement in most growth medium) but also in vivo and especially during the transformation and progression of tumors.…”

mentioning

confidence: 99%

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Lack of Fetuin-A (α2-HS-Glycoprotein) Reduces Mammary Tumor Incidence and Prolongs Tumor Latency via the Transforming Growth Factor-β Signaling Pathway in a Mouse Model of Breast Cancer

Guillory

Sakwe

Saria

et al. 2010

The American Journal of Pathology

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The present analyses were done to define the role of fetuin-A (Fet) in mammary tumorigenesis using the polyoma middle T antigen (PyMT) transgenic mouse model. We crossed Fet-null mice in the C57BL/6 background with PyMT mice in the same background and after a controlled breeding protocol obtained PyMT/Fet ؉/؉ , PyMT/Fet ؉/؊ , and PyMT/Fet ؊/؊ mice that were placed in control and experimental groups.

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α2HS-glycoprotein, an Antagonist of Transforming Growth Factor β In vivo , Inhibits Intestinal Tumor Progression

Abstract: ABSTRACT

Cited by 89 publications

References 47 publications

Discovery of the serum biomarker proteins in severe preeclampsia by proteomic analysis

Discovery of the serum biomarker proteins in severe preeclampsia by proteomic analysis

2‐D difference gel electrophoresis of the lung squamous cell carcinoma versus normal sera demonstrates consistent alterations in the levels of ten specific proteins

Lack of Fetuin-A (α2-HS-Glycoprotein) Reduces Mammary Tumor Incidence and Prolongs Tumor Latency via the Transforming Growth Factor-β Signaling Pathway in a Mouse Model of Breast Cancer

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