α-Smooth muscle actin is expressed in a subpopulation of cultured and cloned fibroblasts and is modulated by γ-interferon

Desmoulière, Alexis; Rubbia‐Brandt, Laura; Abdiu, Avni; Walz, Thomas; Macieira‐Coelho, A.; Gabbiani, Giulio

doi:10.1016/0014-4827(92)90348-c

Cited by 202 publications

(117 citation statements)

References 54 publications

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“…Isoxazole may also act independently of MRTF-A in the setting of a healing wound such that stimulation of Erk1/2 signaling by isoxazole contributes to wound closure by promoting the growth response. Alternatively, suppression of the inflammatory response, as demonstrated by reduced TNF-α expression, might contribute to the accumulation of granulation tissue in the isoxazole-treated wound (42,43). To fully elucidate the role of MRTFs in wound healing, deletion of MRTF-A and -B with both fibroblast-and keratinocyte-specific Cre drivers will likely be necessary.…”

Section: Discussionmentioning

confidence: 99%

Activation of MRTF-A–dependent gene expression with a small molecule promotes myofibroblast differentiation and wound healing

Velasquez

Sutherland²,

Liu³

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

123

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Significance Myofibroblasts are contractile smooth muscle-like cells that control tissue repair and remodeling. Inappropriate myofibroblast differentiation can cause organ fibrosis or inefficient wound healing. In this article, we demonstrate that myocardin-related transcription factor (MRTF)-A is necessary for myofibroblast differentiation. We also identify an isoxazole ring-containing small molecule that stimulates MRTF-A–dependent gene expression, myofibroblast differentiation, and wound healing. These findings suggest that targeting MRTFs pharmacologically may prove useful in treating fibrotic diseases.

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Section: Discussionmentioning

confidence: 99%

Activation of MRTF-A–dependent gene expression with a small molecule promotes myofibroblast differentiation and wound healing

Velasquez

Sutherland²,

Liu³

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

123

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“…Similarly, IL-6 has been implicated in the development of fibrosis [16]. On the other hand, IFN-c is an antifibrotic cytokine [13]. Chou et al [10] have shown that TNF-a inhibits adherence and phagocytosis of collagen; they suggest that inhibition of the collagen phagocytic pathway may contribute to fibrosis.…”

Section: Discussionmentioning

confidence: 99%

Therapeutic Interventions in Oral Submucous Fibrosis: An Experimental and Clinical Study

Singh

Mohammad

Pant³

et al. 2014

J. Maxillofac. Oral Surg.

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Introduction Oral submucous fibrosis (OSF) is a chronic debilitating disease and premalignant condition of the oral cavity and is a serious public health issue in India and many parts of the world. The treatment is still elusive and empirical because of poorly understood etiopathogenesis, which is believed to be multifactorial including areca nut chewing, ingestion of chillies, genetic and immunologic processes, nutritional deficiencies, and many others. The present investigations was focused to understand the possible therapeutic interventions of anti-OSF agents in arecoline induced experimental in vitro model of OSF and clinical application of these anti-OSF agents in the restoration of various grade of the disease. Materials and Methods The 127 subjects were selected from patients who visited the OPD of Department of Oral and Maxillofacial Surgery, Faculty of Dental Sciences, K.G. Medical University, Lucknow. Further the subjects were divided in two groups on the basis of clinical examination. Group-1 subjects showed presence of fibrosis bands in the labial and/or buccal mucosa, loss of elasticity, difficulty to open the mouth and had a habit chewing arecanut in some form. Group-2 subjects had no habit of chewing areca-nut, were apparently healthy with no mucosal disorder. The samples were collected and were immediately transported to Indian Institute of Toxicology Research, Lucknow, for isolation and cultivation of primary cultures of mucosal fibroblast cells. Then isolation and cultivation of oral mucosal fibroblast, identification of non-cytotoxic doses of arecoline, real time PCR, immunocytochemistry, cytokine determination in culture cells, western blot analyses, functional activity of collagenase, lysyl oxidase enzyme activity, collagen beads assay, cyclooxygenase (COX-2) expression analysis was done. Results and Conclusions This study, shows that the reduction of phagocytic cells was strongly related to the arecoline levels in fibroblast culture when we exposed arecoline to normal oral mucosal cells (NOMC) and cells from OSF patient. An enhancement of phagocytic cells was observed following the pre exposure of cells to 1 lM dexamethasone, a glucocorticoids, In this study, histologic evidence is presented which supports the finding that COX-2 expression is upregulated in OSF specimens compared to normal oral submucosal cells. Strong immunostaining for COX-2 was detected in arecoline exposed NOMC and cells from OSF patient. Areca nut extract up-regulates prostaglandin production, cyclooxygenase-2 mRNA and protein expression of human oral keratinocytes. The number of phagocytic cells and phagocytic activity in cultured human oral fibroblasts from OSF site was lower than the fibroblasts from the normal regions of the same person.

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“…In an attempt to induce PLA smooth muscle differentiation, we controlled cell proliferation. Heparin has been shown to prevent proliferation, as well as to induce ASMA expression (25,26). Phenotypic modulation of SMC by extracellular matrix has also been documented (1, 2, 27-29).…”

Section: Discussionmentioning

confidence: 99%

Clonogenic multipotent stem cells in human adipose tissue differentiate into functional smooth muscle cells

Rodríguez¹,

Alfonso²,

Zhang³

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

281

232

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α-Smooth muscle actin is expressed in a subpopulation of cultured and cloned fibroblasts and is modulated by γ-interferon

Cited by 202 publications

References 54 publications

Activation of MRTF-A–dependent gene expression with a small molecule promotes myofibroblast differentiation and wound healing

Activation of MRTF-A–dependent gene expression with a small molecule promotes myofibroblast differentiation and wound healing

Therapeutic Interventions in Oral Submucous Fibrosis: An Experimental and Clinical Study

Clonogenic multipotent stem cells in human adipose tissue differentiate into functional smooth muscle cells

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