α-Myosin heavy chain cDNA structure and gene expression in adult, fetal, and premature baboon myocardium

Hixson, James E.; Henkel, Richard D.; Britten, Marjorie L.; Vernier, D. T.; deLemos, Robert A.; VandeBerg, John L.; Walsh, Richard A.

doi:10.1016/0022-2828(89)90805-5

Cited by 9 publications

(3 citation statements)

References 30 publications

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“…However the shift toward increased α-MHC, a more energy demanding and greater force generating isoform, is likely a compensatory mechanism by which the mice are able to maintain homeostasis is the face of contractile deficiency. [43], [44] …”

Section: Discussionmentioning

confidence: 99%

Systemic Maternal Inflammation and Neonatal Hyperoxia Induces Remodeling and Left Ventricular Dysfunction in Mice

et al. 2011

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AimsThe impact of the neonatal environment on the development of adult cardiovascular disease is poorly understood. Systemic maternal inflammation is linked to growth retardation, preterm birth, and maturation deficits in the developing fetus. Often preterm or small-for-gestational age infants require medical interventions such as oxygen therapy. The long-term pathological consequences of medical interventions on an immature physiology remain unknown. In the present study, we hypothesized that systemic maternal inflammation and neonatal hyperoxia exposure compromise cardiac structure, resulting in LV dysfunction during adulthood.Methods and ResultsPregnant C3H/HeN mice were injected on embryonic day 16 (E16) with LPS (80 µg/kg; i.p.) or saline. Offspring were placed in room air (RA) or 85% O2 for 14 days and subsequently maintained in RA. Cardiac echocardiography, cardiomyocyte contractility, and molecular analyses were performed. Echocardiography revealed persistent lower left ventricular fractional shortening with greater left ventricular end systolic diameter at 8 weeks in LPS/O2 than in saline/RA mice. Isolated cardiomyocytes from LPS/O2 mice had slower rates of contraction and relaxation, and a slower return to baseline length than cardiomyocytes isolated from saline/RA controls. α-/β-MHC ratio was increased and Connexin-43 levels decreased in LPS/O2 mice at 8 weeks. Nox4 was reduced between day 3 and 14 and capillary density was lower at 8 weeks of life in LPS/O2 mice.ConclusionThese results demonstrate that systemic maternal inflammation combined with neonatal hyperoxia exposure induces alterations in cardiac structure and function leading to cardiac failure in adulthood and supports the importance of the intrauterine and neonatal milieu on adult health.

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Section: Discussionmentioning

confidence: 99%

Systemic Maternal Inflammation and Neonatal Hyperoxia Induces Remodeling and Left Ventricular Dysfunction in Mice

et al. 2011

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“…APOE genotypes were encoded as 1 = ε2/ε3, 2 = ε3/ε3, and 3 = ε3/ε4. APOC3 genotypes were determined by PCR and allele-specific restriction digestion of the amplified products with the restriction enzyme SstI following the procedures described elsewhere [ 30 ]. APOC3 genotypes were encoded as 1 = S1/S1 and 2 = S1/S2 (rs5128).…”

Section: Methodsmentioning

confidence: 99%

Birth weight and blood lipid levels in Spanish adolescents: Influence of selected APOE, APOC3 and PPARgamma2 gene polymorphisms. The AVENA Study

et al. 2008

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Background: There is increasing evidence indicating that genes involved in certain metabolic processes of cardiovascular diseases may be of particular influence in people with low body weight at birth. We examined whether the apolipoprotein (APO) E, APOC3 and the peroxisome proliferator-activated receptor-γ-2 (PPARγ2) polymorphisms influence the association between low birth weight and blood lipid levels in healthy adolescents aged 13-18.5 years.

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“…We have previously described marked changes in left ventricular function between d 1 and 3 of life in the premature baboon (3 1) that are associated with improvement in pulmonary and renal function (32). Moreover, induction of a-myosin heavychain synthesis in ventricular myocardium of premature baboons has been demonstrated to occur over the first 10 d of life (33).…”

Section: Age (Hours)mentioning

confidence: 99%

High-Frequency Oscillatory Ventilation Versus Intermittent Mandatory Ventilation: Early Hemodynamic Effects in the Premature Baboon with Hyaline Membrane Disease

Clark

et al. 1991

Pediatr Res

101

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ABSTRACT. We studied the hemodynamic consequences during the first 24 h of life in premature baboons (140 d) with hyaline membrane disease that were treated with high-frequency oscillatory ventilation (HFOV) or conventional intermittent mandatory ventilation (IMV). Cardiac output and organ blood flow were measured at three timepoints using the radiolabeled microsphere technique. Seven of seven HFOV and six of eight IMV animals survived_the 24-h period. By design, initial mean airway pressus (P,,) was higher in the HFOV group (p < 0.01). HFOV Pa, was progressively reduced during the study period because of improving oxygenation as measured by the arterial to alveolar oxygen ratio. In contrast, it was necessary to increase Paw in the IMV animals to maintain the arterial to alveolar oxygen ratio. By 23 h, the IMV group required higher Pa, than the HFOV group (p < 0.05) and had a lower arterial to alveolar oxygen ratio (p < 0.05). We found no significant differences in left ventricular output, effective systemic flow, organ blood flow, or central venous pressure between the two groups at 3, 8, or 23 h. The HFOV strategy used in our study resulted in significant improvement in oxygenation during the initial 24 h of treatment without adverse effect on left ventricular output, cerebral blood flow, or central venousjressure. We conclude that when appropriate changes in Pa, are made during HFOV in response to improvement in arterial oxgenation and changes in lung inflation as assessed by chest radiographs HFOV can be achieved without depressing cardiovascular dynamics more than during conventional therapy with IMV. (Pediatr Res 29: 160-166,1991) Abbreviations CVP, central venous pressure Fi02, fraction of inspired oxygen HFOV, high-frequency oscillatory ventilation HMD, hyaline membrane disease IMV, intermittent mandatory ventilation LVO, left ventricular output -Paw, mean airway pressure PEEP, positive end expiratory pressure PIP, peak inspiratory pressure

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α-Myosin heavy chain cDNA structure and gene expression in adult, fetal, and premature baboon myocardium

Cited by 9 publications

References 30 publications

Systemic Maternal Inflammation and Neonatal Hyperoxia Induces Remodeling and Left Ventricular Dysfunction in Mice

Systemic Maternal Inflammation and Neonatal Hyperoxia Induces Remodeling and Left Ventricular Dysfunction in Mice

Birth weight and blood lipid levels in Spanish adolescents: Influence of selected APOE, APOC3 and PPARgamma2 gene polymorphisms. The AVENA Study

High-Frequency Oscillatory Ventilation Versus Intermittent Mandatory Ventilation: Early Hemodynamic Effects in the Premature Baboon with Hyaline Membrane Disease

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