α-Mannosidase as a Zinc-dependent Enzyme

Snaith, Sybil M.; Levvy, G. A.

doi:10.1038/218091a0

Cited by 34 publications

(19 citation statements)

References 11 publications

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“…This indicates a decline in liver function, which may be due to the anorectic condition of the animals. As expected, there was a decrease in SAP enzyme, which has also been reported by several workers (Snaith and Leuvy, 1968;Parisi and Vallee, 1969). Increase in the level of serum ceruloplasmin with supplementation in group A was more pronounced than in animals of group B (not provided ZnSO 4 ).…”

Section: Discussionsupporting

confidence: 77%

Therapeutic Efficacy of Zinc Sulphate Used in Clustered Model Treatment in Alleviating Zinc Deficiency in Cattle and Its Effect on Hormones, Vitamins and Production Parameters

Sharma

Joshi

2005

Vet Res Commun

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A survey was conducted in 10 districts of northern India. Significant deficiency of zinc was observed in soil, fodders and (cattle) serum samples. The animals showed typical signs of zinc deficiency, namely stiff gait, swelling of hocks and knees, subcutaneous fluid accumulation, rough coat, etc. of variable intensity. A clustered model therapeutic trial was conducted and zinc-deficient cattle were divided into three groups. Group A was provided with mineral mixture containing zinc sulphate. Group B was given mineral mixture without zinc sulphate and group C was given no mineral mixture. Significant improvement (p< 0.01) was observed in the haemoglobin (Hb), total white blood cells (WBC) and total erythrocyte count (TEC) levels at the 7th day of treatment in the animals of group A. Significant improvement in enzyme serum alkaline phosphatase (SAP) was observed in group A animals at the 7th day, while improvement in asparatate aminotransferase (AST), alanine aminotransferase (ALT) and ceruloplasmin (Cp) was observed after 21 days of treatment. Regarding hormones, significant improvement was observed in thyroxine (T3) and triiodothyronine (T4), oestrogen and progesterone in group A animals within 14 days of treatment. The values of vitamin A showed a highly significant (p < 0.01) improvement within 7 days of treatment in group A animals and that of vitamin E on the 21 st day of treatment. The milk yield of lactating cattle and body weight of growing calves in group A showed highly significant (p < 0.01) increases at about 14 and 30 days, respectively. It is concluded that zinc sulphate supplementation is highly effective in alleviating zinc deficiency and improving various biochemical and production parameters in cattle. The clustered model treatment provides a better indicator of the most limiting element under field conditions where simultaneous deficiency of various minerals is prevalent.

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Section: Discussionsupporting

confidence: 77%

Therapeutic Efficacy of Zinc Sulphate Used in Clustered Model Treatment in Alleviating Zinc Deficiency in Cattle and Its Effect on Hormones, Vitamins and Production Parameters

Sharma

Joshi

2005

Vet Res Commun

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“…The activity can be restored immediately by the addition of excess zinc. Thus, the loss of Zn2' during purification may in some cases account for the poor recoveries [e.g., see (521)l and differences in purity and in incubation conditions may explain controversial results on ZnZ + requirement of the enzyme (519,(522)(523)(524)526,527,541). In the presence of 1-mM Zn" , a-mannosidase is stable at 65"C, pH 6.0 for at least 1 h (537).…”

Section: G A-mannosidasementioning

confidence: 99%

Glycolipid and Glycoprotein Degradation

Conzelmann

Sandhoff

1987

Advances in Enzymology - And Related Areas of Molecular Biology

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“…This is not surprising, since the zinc dependence of mammalian a-mannosidase has been previously established (Snaith and Levvy 1968). This is not surprising, since the zinc dependence of mammalian a-mannosidase has been previously established (Snaith and Levvy 1968).…”

Section: Discussionmentioning

confidence: 82%

An IMPROVED FLUORIMETRIC DETERMINATION OF Α‐MANNOSIDASE ACTIVITY IN BOVINE PLASMA

Allen³

1979

Aust Veterinary J

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This paper describes a manual fluorimetric method for the assay of acidic alpha-mannosidase activity in bovine plasma. The optimum conditions for the assay of this enzyme were studied. The assay method devised includes the addition of zinc to the substrate, which stimulates activity by approximately twofold, and reduces the optimum substrate concentration. This latter feature affords considerable cost saving in each test. We have also shown that the alpha-mannosidase activity in lithium heparin plasma, EDTA plasma and blood serum is the same whether the plasma/serum is separated from the cells/clot at 6, 20 or 25 hours after sample collection. This has eliminated the previous necessity of having to deliver whole blood samples to the laboratory within 6 hours of collection. Furthermore samples for the supplementary neutrophil assay can now be taken at the same time as those for the plasma test, and both samples forwarded together. The plasma alpha-mannosidase assay is a rapid and reliable screening test for the mannosidosis genotype and for detecting carrier animals. Carrying out this plasma assay in conjunction with the more definitive neutrophil assay provides a reliable method of distinguishing homozygotes and heterozygotes from normal animals.

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α-Mannosidase as a Zinc-dependent Enzyme

Cited by 34 publications

References 11 publications

Therapeutic Efficacy of Zinc Sulphate Used in Clustered Model Treatment in Alleviating Zinc Deficiency in Cattle and Its Effect on Hormones, Vitamins and Production Parameters

Therapeutic Efficacy of Zinc Sulphate Used in Clustered Model Treatment in Alleviating Zinc Deficiency in Cattle and Its Effect on Hormones, Vitamins and Production Parameters

Glycolipid and Glycoprotein Degradation

An IMPROVED FLUORIMETRIC DETERMINATION OF Α‐MANNOSIDASE ACTIVITY IN BOVINE PLASMA

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