α-lipoic acid prevents buthionine sulfoximine-induced cataract formation in newborn rats

Maitra, Indrani; Serbinova, E. A.; Trischler, Hans; Packer, Lester

doi:10.1016/0891-5849(94)00195-p

Cited by 72 publications

(33 citation statements)

References 27 publications

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“…There is no doubt that the potent free radical scavenger and metal chelator dl-a-lipoic acid [67] counteracts oxidative stress far more effectively than taurine. In addition, in biological tissues dl-a-lipoic acid is reduced to dihydrolipoate, which combines free radical (hydroxyl, superoxide, peroxyl, singlet oxygen) scavenging and metal chelating properties with the ability to regenerate concentrations of non-enzymatic and enzymatic antioxidants [34,67,68]. Our previous study suggests that the antioxidant effects of dl-a-lipoic acid in the retina of streptozotocin-diabetic rats are caused by free radical scavenging and metal chelating proper- ties rather than to changes in GSH and GSSG concentrations and the glutathione redox state [34].…”

Section: Discussionmentioning

confidence: 99%

Antioxidants attenuate early up regulation of retinal vascular endothelial growth factor in streptozotocin-diabetic rats

et al. 2001

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Aims/hypothesis. A strong positive correlation has been found between lipid peroxidation product and vascular endothelial growth factor concentrations in the vitreous of patients with proliferative diabetic retinopathy. To establish a causal relation between diabetes-associated enhanced oxidative stress and vascular endothelial growth factor production, we evaluated two antioxidants, dl-a-lipoic acid and taurine, on retinal vascular endothelial growth factor protein and mRNA expression and on parameters of oxidative stress in streptozotocin-diabetic rats. Methods. Our experiments were on control rats and streptozotocin-diabetic rats with a 6-week duration of diabetes, treated with or without dl-a-lipoic acid (100 mg´kg ±1´d±1 , i. p.) or taurine (1 % in the diet) starting from induction of diabetes. Vascular endothelial growth factor protein in retinal homogenates was assessed by sandwich ELISA with an affinity-purified polyclonal antibody and vascular endothelial growth factor mRNA by ribonuclease protection assay. Retinal lipid peroxidation products i. e. malondialdehyde plus 4-hydroxyalkenals were quantified with n-methyl-2-phenylindole. Retinal reduced and oxidized glutathione, ascorbate, dehydroascorbate, and sorbitol pathway intermediates were measured spectrofluorometrically, and taurine by reverse-phase HPLC.Results. Vascular endothelial growth factor protein concentration (means SD) was increased in diabetic rats compared with control rats (33 7 vs 19 5 pg/mg total protein, p < 0.01) This increase was attenuated by taurine (26 8, p < 0.05) and prevented by dla-lipoic acid (21 4, p < 0.01). Vascular endothelial growth factor mRNA abundance was reduced by 1.4-fold in diabetic rats compared with control rats and this decrease was attenuated but not completely prevented by both antioxidants. Malondialdehyde plus 4-hydroxyalkenal concentration was increased in diabetic rats compared with control rats, and both antioxidants arrested accumulation of lipid peroxidation products. Taurine, reduced glutathione, oxidized glutathione, ascorbate, dehydroascorbate and sorbitol pathway intermediate concentrations as well as oxidized glutathione/reduced glutathione and dehydroascorbate/ascorbate ratios were similar in control and diabetic rats treated with or without taurine. Conclusion/interpretation. Oxidative stress is directly involved in up regulation of vascular endothelial growth factor protein in the retina during early diabetes. [Diabetologia (2001

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Section: Discussionmentioning

confidence: 99%

Antioxidants attenuate early up regulation of retinal vascular endothelial growth factor in streptozotocin-diabetic rats

et al. 2001

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“…These studies suggest that the combination of both antioxidants can be beneficial in limiting pathological processes in which excessive production of oxidant radicals may be involved in the initiation and progression of damage (e.g., diabetic neuropathy, Alzheimer's disease, rheumatic arthritis, cardiac and cerebral ischemia), and in the aging process [19,23]. ALA markedly modifies in situ concentrations of VE [32,33], which suggests a possible synergistic effect of the two compounds.…”

Section: Synergism Between Lipoate and Tocopherolmentioning

confidence: 99%

“…To explain the synergy between ALA and VE, it has been proposed that alpha-tocopherol is regenerated via lipoate [32,33,37]. Nevertheless, this phenomenon, by itself, cannot explain the magnitude of findings.…”

Section: Synergism Between Lipoate and Tocopherolmentioning

confidence: 99%

Therapeutic perspectives on the combination of α-lipoic acid and vitamin E

González-Pérez

González-Castañeda

2006

Nutrition Research

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Alpha-lipoic acid and vitamin E have synergistic effects, as determined in models of oxidant radical lesions. This review summarizes recent findings showing that the combination of alpha-lipoic acid plus vitamin E has beneficial effects in reducing oxidative damage in ischemic or other oxidationrelated pathological events. Both antioxidants are common in the normal human diet and side effects are very rare. Therefore, alpha-lipoic acid and vitamin E can counteract oxidative processes and could have an important role in clinical medicine.

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“…Lipoic acid has been used in treating liver cirrhosis, atherosclerosis and polyneuritis of diabetes mellitus, diseases in which oxidative stress plays a role. Normally α-lipoic acid is an endogenous thiol that is present in very small amounts (5–25 nmol/g) in mammalian tissues, usually bound to enzyme complexes and unavailable as an antioxidant [5]. General biological effects of lipoic acid include the quenching of reactive oxygen species, metal chelation, interaction and regeneration of other antioxidants such as ascorbate and vitamin E, redox regulation of protein thiol groups with an influence in protein folding and effects on gene expression and apoptosis [6].…”

Section: Introductionmentioning

confidence: 99%

The Protective Effect of Alpha-Lipoic Acid against Oxidative Damage in Rabbit Conjunctiva and Cornea Exposed to Ultraviolet Radiation

2005

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Purpose: The purpose of this study was to determine the protective effect of α-lipoic acid against oxidative damage in rabbit conjunctiva and cornea exposed to ultraviolet radiation. Methods: 20 rabbits weighing 2,500– 3,000 g were used, and we divided them into 4 groups with 5 randomly selected rabbits. The rabbits were exposed to 2 J/cm²/h of ultraviolet A radiation (UVA) in the range of 320–405 nm for 12 h per day within 90 days. The control group did not undergo any procedure, the UVA group was only exposed to UVA radiation. The PUVA group was treated with 8-methoxypsoralen and UVA. The α-lipoic acid group was administered 8-methoxypsoralen + UVA + α-lipoic acid. At the end of 90 days, the rabbits were killed by decapitation, and the eyes were enucleated. Both eyes of each rabbit were used for biochemical evaluation. Conjunctival and corneal free malondialdehyde (MDA), glutathione peroxidase (GSH-PX) and superoxide dismutase (SOD) levels were compared among the groups. Results: Conjunctival free MDA levels were lower in the α-lipoic acid group compared with the UVA and PUVA groups (p < 0.05, p < 0.001, respectively). Both conjunctival SOD levels (p < 0.05, p < 0.01, respectively) and conjunctival GSH-PX levels (p < 0.01, p < 0.001, respectively) were higher in the α-lipoic acid group compared with other groups. Corneal free MDA levels were lower in the α-lipoic acid group compared with the UVA and PUVA groups (p < 0.01, p < 0.001, respectively). Both corneal SOD levels (p < 0.01, p < 0.01, respectively) and corneal GSH-PX levels (p < 0.01, p < 0.01, respectively) were higher in the α-lipoic acid group compared with the other groups. Conclusion: α-Lipoic acid which is considered as potent antioxidant protects the eye from the damaging effect of ultraviolet exposure.

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α-lipoic acid prevents buthionine sulfoximine-induced cataract formation in newborn rats

Cited by 72 publications

References 27 publications

Antioxidants attenuate early up regulation of retinal vascular endothelial growth factor in streptozotocin-diabetic rats

Antioxidants attenuate early up regulation of retinal vascular endothelial growth factor in streptozotocin-diabetic rats

Therapeutic perspectives on the combination of α-lipoic acid and vitamin E

The Protective Effect of Alpha-Lipoic Acid against Oxidative Damage in Rabbit Conjunctiva and Cornea Exposed to Ultraviolet Radiation

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